گروه زیست شناسی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران
چکیده
کلرپیریفوس یکی از حشرهکشهای ارگانوفسفره است که در سال‌های اخیر به دلیل عوارض مخرب فراوان بر روی پوست، دستگاه عصبی، تنفسی و گوارشی بیشتر مورد مطالعه قرار گرفته است. اما تا کنون مطالعهای از اثر این سم بر روی جنین در دسترس نیست. بنابراین در این پژوهش به بررسی اثرات تراتوژنیک کلرپیریفوس بر روی رشد و نمو کمی جنینهای موش Balb/C پرداختیم. در این مطالعه 50 سر موش بطور تصادفی به 6 گروه مساوی، کنترل (عدم تزریق: پنج سر موش)، شم (تزریق سرم فیزیولوژی: پنج سر موش) و چهار گروه تجربی (10 سر موش در هر گروه) تقسیم شدند. دوز کشنده LD50 در شرایط in vivo، 32/25 میلیلیتر بر کیلوگرم در وزن بدن تعیین و دوز تزریقی 4/0 میلیلیتر بر کیلوگرم انتخاب شد. تزریقات برای 4 گروه موش در روزهای 3، 4، 5 و 6 بارداری بصورت درون صفاقی انجام و موشها در روز 15 بارداری تشریح شدند و برای اطمینان، تجربیات فوق2 بار تکرار شد. دادهها با نرمافزار SPSS17 از طریق درصدگیری مورد سنجش قرار گرفتند. پس از یک مطالعه تطبیقی در این دوز، اثرات تراتوژنیک مختلفی از جمله اگزوهپاتیک، اگزانسفالی، سینداکتلی، نقص در اندامهای حرکتی (دست و پا)، خونریزی وسیع در کل بدن و اگزوفتالمی در مقایسه با گروه کنترل و شم مشاهده شد. با توجه به یافتههای فوق، مصرف کلرپیریفوس به عنوان یکی از سموم کشاورزی، تاثیرات منفی بر روی جنین دارد و یک تراتوژن است. بنابراین توصیه میشود برای حفظ محیط زیست و سلامتی انسانها، مخصوصاً خانم های باردار از روشهای غیرشیمیایی برای کنترل آفات استفاده شود.
The Teratogenic Effects Pesticide of Chlorpyrifos on the Development of BALB/c Mouse Embryos 3Th to 6Th Days of Pregnancy
نویسندگان [English]
S. Rastgar Gharahshiran؛ P. Torabzadeh؛ S. Saffari
Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran
چکیده [English]
Chlorpyrifos is one of organophosphate insecticides and had studied in recent years because it has many complications devastating on skin, nervous, respiratory and digestive systems, but its effects on the embryo especially during pregnancy has not been reviewed. Therefore, in study the teratogenic effects pesticide of Chlorpyrifos on the development of BALB/c mouse embryos 3rd to 6thd of pregnancy. In this study, 50 female BALB/c mouse were randomly divided into 6 equal groups a control group (non-injection: 5 mice) and witness (injection of saline: 5 mice) and 4 experimental groups (each group: 10 mice). A lethal dose LD50 was determined in condition of 25.32 ml/kg.bw in vivo and selected dose for injection 0.4 ml/kg.bw. Injection was done on the 3rd to 6th d of pregnancy days by enema. Then the mouse was sacrificed on day 15 of pregnancy. For security of results above experiences was repeated 2 times. Data were checked with SPSS 17 software by percentage. After a comparative study at this dose has been observed a significant increase, extrahepatic, exencephaly, syndactyly, having a defect in dynamic organs (legs and hands), extensive bleeding in whole body and exophthalmia were compared with control and witness groups.According to the findings, negative effects of Chlorpyrifos use as pesticides in agricultural products. On the mouse embryos, it is recommended to protect the environment and human health, especially pregnant women use of non-chemical methods to control pests.
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