1گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
2گروه زیست‌شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
3گروه زیست شناسی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
چکیده
مصرف فراورده های گیاهی و استفاده از داروهای گیاهی امروزه از پر طرفدارترین روش های درمان به شمار میرود .در این بین جینسنگ گیاهی از جنس panax و از خانواده Araliaceae جزء ده گیاه رایج در سبد غذایی مردم قرار گرفته است. هدف از این مطالعه بررسی اثر مصرف عصاره جینسنگ بر اووژنز در موش بالغ نژاد NMRI بود .در این تحقیق 30 سر موش ماده به طور تصادفی در 5 گروه کنترل (مورد گاواژ قرار نگرفتند)، شم (مورد گاواژ آب مقطر قرار گرفتند) و سه گروه تجربی (تحت گاواز 100، 200 و 300 میلیگرم بر کیلوگرم وزن موش از عصاره آبی جینسنگ) قرار داده شدند. عصاره آبی جینسنگ قرمز در آب مقطر تهیه گردید و گرو ها طبق گروه بندی ذکر شده به مدت 30 روز تحت تیمار بودند. پس از آن به روش نخاعی کشته شدند، بافت تخمدان و رحم آنها خارج گردید و مورد مطالعه قرار گرفت .تعداد فولیکولهای اولیه در گروههای تجربی با دوز 100 200 و 300 میلیگرم بر کیلوگرم افرایش معنی دار را نشان دادوتعداد فولیکول های ثانویه در دوز 100 و 200 میلیگرم بر کیلوگرم و فولیکول گراآف در دوز 200 میلیگرم بر کیلوگرم افزایش معنی دار را نشان دادند. کاهش در تعداد فولیکولهای آترتیک در کمترین و بیشترین دوز مورد استفاده مشاهده گردید. مطالعات رحمی افزایش ضخامت لایه پریمتر و آندومتر را در گروه با دوز 100 را نشان داد و تعداد غدد ترشحی در گرو های تجربی 1و2 افزایش داشت. در نهایت میتوان گفت مصرف جینسنگ در راستای بهبود عملکرد تخمدان وبافت رحم عمل میکند و به اووژنز کمک میکند.
The Effect of Red Ginseng Water Extract on Oogenesis and Uterus Tissue of Adult NMR Mice
نویسندگان [English]
Z. Zohrevand Asl1؛ N. hayati roudbari2؛ S. Mohamadi Gorji3؛ K. Parivar1
1Department of Biology, Science and Research Branch, Isamic Azad University, Tehran, Iran
2Department of Biology, Science and Research Branch, Isamic Azad University, Tehran, Iran
3Department of Biology, Sari Branch, Islamic Azad University, Tehran, Iran
چکیده [English]
Herbal product consumption and use of herbal medicines are now the most popular treatment methods. In the meantime, ginseng belongs to the genus Panax of the family Araliaceae has been among top ten common plants in people's food basket. The objective of this study was to evaluate the effect of ginseng extract on oogenesis in the adult NMRI strain. In this experimental study, we randomly divided 30 adult female mouse NMRI strain into 5 groups for 30 d as follows: 1) Control (not gavaged), 2) Sham (gavaged water), 3) Treatment groups (gavaged 100 mg/kg, 200 mg/kg and 300 mg/kg ginseng extract). Ginseng water extract was prepared in distilled water. After 30 d, they were killed. Ovarian and uterine tissue were removed and studied.The number of primary follicles in three experimental groups was 100, 200 and 300 mg/kg. Secondary follicles with doses of 100 and 200 mg/kg groups and graaf follicle with dose of 200 mg/kg group showed significant increase. Reduction in atretic follicle in groups with doses of 100 and 300 mg/kg was observed. Uterine studies showed significant increase in the thickness of perimeter and endometrial layer in group with dose of 100 mg/kg and the number of glands in experimental groups 1 and 2 showed increased.Finally, wecan say that using red ginseng water extract is effective on oogenesis and uterus tissue.
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