گروه زیست شناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران
چکیده
ﺑﻴﻤﺎری ﭘﺎرﻛﻴﻨﺴﻮن ﻳﻚ اﺧﺘﻼل ﻧﻮروﭘﺎﺗﻮﻟﻮژﻳﻚ ﺷﺎﻳﻊ اﺳﺖ ﻛﻪ ﺑـﻪ ﻋﻠﺖ دژﻧﺮاﺳﻴﻮن ﻧﻮرونﻫﺎی دوﭘﺎﻣینرژﻳﻚ ﺑﺨﺶ ﻣﺘﺮاﻛﻢ ﺟﺴﻢ ﺳﻴﺎه اﻳﺠﺎد ﻣﻲﺷﻮد. استرس اکسیداتیو به عنوان یک عامل اصلی ایجاد این بیماری محسوب میشود که موجب مرگ نورونها می شود. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺧﺎﺻﻴﺖ آنتیاکسیدانی الاژیک اسید، هدف مطالعه حاضر بررسی اﺛﺮ الاژیک اسید بر اختلالات حرکتی و شناختی در ﻣﺪل حیوانی ﺑﻴﻤﺎری ﭘﺎرﻛﻴﻨﺴﻮن است. چهل سر موش صحرایی نر به پنج گروه هشتتایی تقسیم شدند. به منظور ایجاد مدل حیوانی پارکینسون موشها، سم عصبی 6- هیدروکسی دوپامین (6-OHDA) را با دوز 8 میکروگرم در 2 میکرولیتر سالین نرمال دارای 01/0 درصد اسید آسکوربیک را درون هسته میانی- قدامی (MFB) سمت چپ مغز دریافت کردند. گروههای درمان الاژیک اسید با دوزهای 10، 25 و 50 میلیگرم بر کیلوگرم را به مدت 14 روز به صورت گاواژ داخل معدی دریافت کردند. یک روز بعد از آخرین گاواژ تستهای حرکتی و حافظه از موشها به عمل آمد. الاژیک اسید باعث بهبود اختلالات حرکتی و حافظه ناشی از پارکینسون بویژه در دوز 50 میلیگرم بر کیلوگرم شد. با توجه به یافتههای حاضر در این پژوهش میتوان پیشنهاد نمود که الاژیک اسید دارای اﺛـﺮ ﺣﻔﺎﻇﺘﻲ در ﺑﺮاﺑـﺮ 6- ﻫﻴﺪروﻛﺴﻲ دوﭘﺎﻣﻴﻦ در ﻣﺪل ﺗﺠﺮﺑﻲ ﺑﻴﻤﺎری ﭘﺎرﻛﻴﻨﺴﻮن اﺳﺖ.
Effect of Ellagic Acid on Motor and Memory Disorders induced by 6-OHDA in Male Rats
نویسندگان [English]
Z. Eydipour؛ M. Rafieirad
Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
چکیده [English]
Parkinson's disease is a prevalent neuropathological disorder induced due to the degeneration of dopaminergic neurons in the dense part of substantia nigra. Oxidative stress is regarded as the main factor of this disease that causes death of neurons. Considering the protective antioxidative property of ellagic acid, the aim of the present study was to investigate the effect of ellagic acid on motor disorders and memory of animal model of Parkinson's disease. Forty male rats were divided into 5 groups. To create an animal model, the rats received 6-OHDA neural poison with dose of 8 µg in 2 µl of normal saline solution with 0.01% of ascorbic acid inside medial forebrain bundle (MFB) on the left side of the brain. The treatment groups received ellagic acid with doses of 10, 25, and 50 mg/kg for 14 d as gastric gavage and tests were conducted on the rats one day after the last gavage. Ellagic acid improves motor and memory disorders motor disorders and memory resulting from Parkinson's disease particularly at doses 50 mg/kg. Considering the present findings, ellagic acid has a protective effect on 6-OHDA in the experimental model of Parkinson's disease.
کلیدواژه‌ها [English]
ellagic acid, Motion, memory, Parkinson's disease
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