بررسی اثر عصاره ترکیبی سیر و لیموترش بر ریسک فاکتور التهابی آترواسکلروزیس (hs-CRP) در رت های نر نژاد ویستار تحت رژیم پرچرب

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مشکلات قلبی عروقی بویژه آترواسکلروزیس یکی از بیماری­های شایع در جهان است. پروتئین فاز حاد (hs-CRP) حساس­ترین شاخص التهابی است که در تحقیقات جدید نشان داده شده مقادیر سرمی آن می­تواند خطر معضلات قلبی را پیش­بینی نماید. سیر (Allium sativum) و لیمو ترش (Citrus limon) هر دو از گیاهانی هستند که در درمان­های سنتی مشکلات قلبی­عروقی کاربرد دارند ولی ترکیب آن دو با یکدیگر تاکنون بررسی نشده است. مطالعه حاضر، یک پژوهش تجربی مداخله­ای است که تاثیرات ضدالتهابی و هیپولیپیدمیک عصاره ترکیبی سیر و لیمو ترش را در موش صحرایی نژاد ویستار که به مدت 9 هفته تحت تغذیه با رژیم پرچرب قرار گرفته­اند، بررسی می­کند. 42 سر رت نر بالغ با وزن 200 تا 250 گرم به شش گروه هفت­تایی تقسیم شدند. گروه کنترل و گروه تجربی 3 با غذای عادی جوندگان و گروه کنترل چاقی، شم، تجربی 1و تجربی 2 با رژیم پر چرب تغذیه شدند. پس از رسیدن به وزن مطلوب، عصاره ترکیبی سیر و لیمو در دوز 220 میلی­گرم ماده خشک محلول در آب مقطر به گروه­های تجربی 1 و 3 و در دوز 440 میلی­گرم به گروه تجربی 2، برای مدت چهار هفته خورانده شد. سرم حاصل از نمونه­گیری خون برای سنجش CRP و کلسترول تام مورد آزمایش قرار گرفت. نتایج با آزمون آنالیز واریانس (ANOVA) و روش مقایسه­های چند گانه توکی مورد آزمون قرار گرفت. عصاره ترکیبی سیر و لیمو کاهش معنی­داری را در کلسترول تام سرم گروه­های تجربی 1 و 2 نشان داد (001/0> p). همچنین یک کاهش معنی­دار CRP در گروه تجربی 2 که با دوز دو برابر تیمار شده بودند دیده شد (05/0 > p). هیچ تغییر معنی­داری از تاثیر عصاره ترکیبی بر روی گروه تجربی 3 که با رژیم غذایی عادی تغذیه شده بودند، مشاهده نگردید. به نظر می­رسد عصاره ترکیبی سیر و لیمو ترش در مبتلایان به هایپرکلسترولمی تاثیرات ضدالتهابی و محافظتی بر عروق دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Combined Extracts of Allium sativum and Citrus limon on Atherosclerosis Inflammatory Risk Factor (hs-CRP) in Male Wistar Rats under High-fat Diet

نویسندگان [English]

  • P. Baradaran Khalkhali
  • S.A. Haeri Rouhani
  • P. Yaghmaee
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Cardiovascular disease, which includes atherosclerosis, is one of the major diseases in the world. High sensitive C-reactive protein is the most sensitive inflammatory marker that recently its serum level predicts risk of cardiovascular disease is suggested. Both garlic (Allium sativum) and lemon (citrus limon) are used in the traditional management of CVD without a scientific evidence of the benefit of their combination. This study designed to investigate the hypolipidemic and anti-inflammatory effects of combined extracts of A. sativum and C. limon in male Wistar rats after high-fat diet for eight weeks. For this purpose, 42 adult male rats of Wistar strain, with weights between 200 and 250 gr, were divided into six groups (n=7). Group control and Exp3 was fed on normal pellet diet while group control fat, Sham, Exp1 and Exp2 fed on high-fat diet for up to 8 wk. The combined extract was administered to rats in graded doses for 4 wk and blood serum was analyzed for C-reactive protein and total cholesterol.Results are expressed as means ± SE. Significance differences of biochemical measurements were analyzed by using repeated measure and ANOVA tests to identify differences in phases and among groups. When appropriate, a Tukey posthoc was applied. Statistical significance was accepted at PA. sativum and C. limon was recognizable. However, future long-term well-designed investigations would provide valuable information to establish public health recommendations on the combined extract, taking into account both the nature of the compounds and the optimal dose, for cardiovascular health protection
 

کلیدواژه‌ها [English]

  • Atherosclerosis
  • C-reactive protein
  • Allium sativum
  • Citrus limon
  • Wistar Rat
  • Hyperlipidemia
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