بررسی اثرات ضددردی و ضدالتهابی عصاره الکلی برگ اسفناج (Spinacia oleraceal) در موش کوچک آزمایشگاهی NMRI

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

چکیده

طبق تحقیقات انجام شده، عصاره اسفناج اثرات فارماکولوژیکی متنوعی از قبیل ضدالتهابی، آنتی‌اکسیدانی و ضداضطرابی دارد. مطالعه حاضر به بررسی اثر ضددردی و ضدالتهابی عصاره اتانولی برگ اسفناج در موش کوچک آزمایشگاهی نر بالغ با استفاده از آزمون فرمالین، صفحه داغ وآزمون زایلن می‌پردازد. در این پژوهش که روی 36  موش کوچک آزمایشگاهی نر بالغ نژاد NMRI با وزن 25 تا30 گرم انجام شد، حیوانات به طور تصادفی به 6 گروه 6تایی شامل: گروه کنترل (سالم)، گروه شاهد (دریافت‌کننده نرمال سالین) و همچنین سه گروه دریافت‌کننده دوزهای 150، 300 و 600 میلی‌گرم بر کیلوگرم وزن بدن عصاره اتانولی برگ اسفناج وگروه استاندارد (دریافت‌کننده دگزامتازون با دوز 10  میلی‌گرم بر کیلوگرم یا مورفین با دوز 10  میلی‌گرم بر کیلوگرم) تقسیم شدند. پس از انجام تیمار حیوانات به وسیله آزمون فرمالین و هات پلیت از نظر واکنش به درد، مورد بررسی قرار گرفتند. همچنن فعالیت ضدالتهابی توسط آزمون زایلن یا التهاب گوش در حیوان بررسی گردید. تمامی داده‌ها از نظر آماری با استفاده از آنالیز واریانس یک‌طرفه و سپس آزمون توکی مقایسه و بررسی شدند. نتایج این مطالعه نشان داد که عصاره اتانولی برگ اسفناج موجب کاهش معنی‌دار درد در فاز مزمن تست فرمالین و تست صفحه داغ می‌شود. همچنین عصاره اتانولی برگ اسفناج درآزمون ادم گوش دارای فعالیت معنادار ضدالتهابی است. تحقیق حاضر نشان داد عصاره اتانولی برگ اسفناج دارای خواص ضددردی و ضدالتهابی در موش کوچک آزمایشگاهی است.

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Antinociceptive and Anti-inflammatory Spinacia oleracea Ethanolic Extract in NMRI Mice

نویسندگان [English]

  • S. Mirazizi 1
  • Vaezi Gh. 2
  • S.A. Haeri Rohani 1
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
چکیده [English]

Spinach has various pharmacological effects such as anti-inflammatory, antioxidant, and anti-stress effects. We aimed to investigate pain killing effects of ethanol essence of spinach leaf in adult male laboratory mice by formalin test and hot plate. This study was done on 36 adult male small laboratory mice (NMRI) race with 25 to 30 gr of weight. The animals were randomly divided into 6 groups of 6 members including control groups (healthy), experimental groups (receiving formal saline) and also three groups receiving doses of 150, 300 and 600 mg/kg body weight ethanol essence, and standard groups (receiving morphine) with a dose of 10 mg/kg body weight. After the treatment, the animals were studied by formalin test and hot plate with regard to reaction to pain. In addition, anti-inflammatory activity was investigated by test in the animal. The data was then analyzed by one-way covariance analysis and then by Tukey test. The ethanol essence of Spinacia oleacea L leaf decreased pain at the chronic phase of formalin test and hot plate. In addition, ethanol essence of spinach leaf had significant anti-inflammatory activity in ear edema test. The ethanol essence in spinach leaf hasantinociceptive and anti-inflammatory activity in mice.

کلیدواژه‌ها [English]

  • Anti-inflammatory
  • Antinociceptive
  • Ethanol essence
  • Mouse
  • Spinacia oleracea
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