بررسی اثر روغن سیاه دانه در درمان زخم معده ناشی از اسید استیک در موش صحرایی نر

نوع مقاله: مقاله پژوهشی

نویسنده

گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

زخم معده یا گاستریت بیماری خوش­خیم در دستگاه گوارش می‌باشد که با زخم کوچک در آستر و مخاط داخلی معده به وجود می­آید. از مشخصات زخم معده این است که عمقی است و از مخاط معده عبور می­کند. تقریباً  تمام زخم­های خوش خیم معده در آنتروم (ناحیه تحتانی معده)، قرار دارد .از گیاهان دارویی موثر در درمان زخم معده سیاه دانه است. هدف از این مطالعه، بررسی اثر روغن سیاه­دانه با سه دوز 50، 100 و 200 میلی­گرم بر کیلوگرم در ترمیم زخم معده ناشی از اسید استیک در موش صحرایی نر می­باشد. موش­ها با رژیم آزمایشگاهی استاندارد تغذیه شده و قبل از جراحی، برای 48 ساعت از غذا محروم و دو ساعت قبل از جراحی، آب آنها نیز قطع شد. موش­ها باتزریق محلول اسید استیک به ناحیه­ی تنه معده قسمتی که فاقد رگ باشد، به زخم معده مبتلا شدند. یک روز پس از ایجاد زخم معده، روغن با دوزهای مختلف از طریق گاواژ به موش­ها داده شد. موش­ها را در روزهای 4، 7، 10 و 14 پس از ایجاد زخم کشته و معده آنها خارج گردید و ابعاد زخم برحسب میلی­متر مربع محاسبه گردید. جهت مطالعات بافت شناسی، نمونه­ها پس از ایجاد برش و تهیه لام با هموتوکسیلین و ائوزین رنگ­آمیزی شدند و مورد مطالعه میکروسکوپی قرارگرفتند. نتایج نشان داد که درصد بهبود زخم در روغن سیاه دانه در هر سه دوز در روزهای چهارم، هفتم، دهم و چهاردهم افزایش معناداری نسبت به گروه­های شم و کنترل دارند. به دلیل وجود ترکیباتی مانند ترپن­ها، فلاونوئیدها، ویتامین A،B  و C موجود در روغن سیاه دانه و خاصیت ضدالتهابی و آنتی­اکسیدانی باعث تسهیل در ترمیم زخم معده، حفاظت از بافت معده و کاهش التهاب و زخم، اثر ترمیمی بیشتری بر زخم معده ناشی از اسید استیک دارد.

کلیدواژه‌ها


عنوان مقاله [English]

An Investigation into the Effect of Nigella Sativa Oil on the Treatment of Gastric Ulcer Induced by Acetic Acid in Male Rats

نویسنده [English]

  • Mahdieh Azarshab
Biology Department, Tehran North Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Gastric ulcer or gastritis is a benign disease in the digestive system which is caused by a small ulcer in the inner lining and gastric mucosa. Among gastric ulcer characteristics is the deep ulcer that passes through the gastric mucosa. Almost all benign gastric ulcers are located in the anthrome (the lower stomach area). One of the methods to treat gastric ulcer is the use of medicinal herbs. Among effective medicinal herbs in treating gastric ulcer is Nigella sativa. The most important application of Nigella sativa is in inflammation reduction and wound healing acceleration. Substances such as linoleic oil, thymoquinone and antioxidants are obtained from nigella sativa. The rats were fed using a standard laboratory diet. Prior to surgery, the rats were deprived of food for 48 hours, and no water was given to them two hours before surgery. Gastric ulcer was generated by injecting acetic acid to stomach lining where there were no vessels. The oil at different doses was given to rats through gavage tube one day after gastric ulcer was generated. The rats were killed on days 4, 7, 10, and 14, and their stomachs were removed and the ulcer dimensions were calculated in terms of m2. Moreover, after cutting and providing lam, in order to conduct histological studies, samples were stained using hematoxylin and eosin and were examined by microscope. The obtained results showed that the percentage of wound healing in Nigella sativa at three doses (50, 100, 200 mg/kg) on days 4, 7, 10, and 14 had a significant increase in comparison to sham-operated and control groups (P<5%). Due to the presence of compounds such as terpenes, flavonoids, vitamins A, B, and C, and the anti-inflammatory and antioxidant properties in Nigella sativa, it facilitates the gastric ulcer healing, protects stomach tissue, and reduces gastric inflammation and ulcer, and has a greater restorative effect on gastric ulcer caused by acetic acid.

کلیدواژه‌ها [English]

  • acetic acid
  • Gastric ulcer
  • Linoleic oil
  • Nigella sativa
  • Rat
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