اثر عصاره پلی ساکارید محلول در آب جلبک پادینا (Padina australis Hauck) بر فعالیت آنزیم های گوارشی و فراسنجه های بیوشیمیایی میگوی پا سفید غربی (Litopenaeus vannamei)


1 دانشگاه دریانوردی و علوم دریایی چابهار

2 موسسه تحقیقات شیلات


در تحقیق حاضر، عصاره پلی ساکارید محلول در آب جلبک پادینا (Padina australis) در غلظت­های 0، 5/0، 1 و 5/1 گرم بر کیلوگرم غذا برای سنجش توانایی عصاره در بهبود فعالیت آنزیم­های گوارشی و شاخص­های بیوشیمیایی به جیره غذایی میگوی پا سفید غربی (Litopenaus vannamei) با میانگین وزنی 1/0 ± 1 گرم اضافه شد. بر طبق تغذیه از این رژیم­های غذایی به­مدت 60 روز، بیشترین فعالیت آنزیم­های پروتئاز و لیپاز در تیمار حاوی 5/1 گرم عصاره پلی­ساکارید جلبک بر کیلوگرم غذا مشاهده شد (05/0 >p ). سطح آلبومین و پروتئین تام در تیمارهای حاوی سطوح مختلف عصاره پلی ساکارید جلبک بیشتر از تیمار شاهد بود (05/0 > p). کمترین میزان گلوکز، تری­گلیسرید، کلسترول، آسپارتات آمینوترانسفراز، آلانین آمینوترانسفراز و آلکالین فسفاتاز در تیمار حاوی 5/1 گرم عصاره پلی ساکارید جلبک بر کیلوگرم غذا مشاهده شد که اختلاف معنی­داری را در مقایسه با سایر تیمارها نشان دادند (05/0 > p). نتایج این مطالعه نشان داد که استفاده از 5/1 گرم عصاره پلی ساکارید جلبک پادینا بر کیلوگرم غذا ممکن است عملکرد هضم، سلامت کبد و سیستم ایمنی غیر اختصاصی میگوی پاسفید غربی را افزایش دهد.


عنوان مقاله [English]

Effect of Water Soluble Polysaccharide Extract of the Algae, Padina australis Hauck, on the Activity of Digestive Enzymes and Biochemical Parameters of White-leg Shrimp, Litopenaeus vannamei

نویسندگان [English]

  • P. Akbary 1
  • Z. Aminikhoei 2
1 Department of Fisheries, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
2 Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Scientific Research Institute (IFSRI), Offshore Research Center, Chabahar, Iran
چکیده [English]

In the present study, the water soluble polysaccharide extract of Padina australis (PPE) was added in concentrations of 0, 0.5, 1 and 1.5 g/kg to the diet of white leg shrimps (Litopenaeus vannamei) with average weight of 1±0.1 g to assess its ability to improve the activity of digestive enzymes and the biochemical parameters. Following feeding on these diets for 60 days, the highest activity of lipase and protease was shown in treatment containing 1.5 g PPE/kg diet (p < 0.05). The levels of albumin and total protein were higher in treatments containing various levels of PPE compared to the control treatment (p < 0.05). The lowest levels of GLU, TRI, CHO, ASP.ALT and ALP were observed in the treatment containing 1.5 g PPE/kg diet indicating significant difference with other treatments (p < 0.05). The results demonstrated that inclusion of 1.5 g/kg diet of PPE in commercial feeds might enhance the digestion function, liver health and nonspecific immunity system of L. vannamei.

کلیدواژه‌ها [English]

  • Polysaccharide extract
  • Padina australis
  • Litopenaeus vannamei
  • Digestive enzymes
  • Biochemical parameters
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