مقایسه دوازده هفته تمرین تناوبی شدید و تداومی با شدت متوسط بر بیان پروتئین‌های میتوکندریاییPGC1α و Tfam در عضله دوقلوی رت‌های سالمند

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

2 مرکز تحقیقات درمان و پیشگیری از چاقی، پژوهشکده علوم غدد درون ریز و متابولیسم، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید بهشتی، تهران، ایران

چکیده

تمرین استقامتی به افزایش پروتئین­ها، آنزیم­ها و عملکرد میتوکندریایی در نمونه­های انسانی و حیوانی منجر می­شود. هدف پژوهش حاضر مقایسه 12 هفته تمرین تناوبی ‌شدید (HIIT) و تداومی با شدت متوسط (MICT) بر بیان پروتئین‌های میتوکندریایی PGC-1a و TFAM در عضله دوقلو رت‌های سالمند بود. در این مطالعه تعداد 45 رت نژاد ویستار سالمند ۲۲ ماهه (۳۲۵-۳۷۵ گرم) تصادفی به سه گروه HIIT، تمرین تداومی با MICT و گروه کنترل تقسیم شدند. تمرین ورزشی در گروه HIIT در هفته اول ۲۰ دقیقه(2 دقیقه با شدت 85 تا 90 درصد VO2max و 2 دقیقه بازیافت (با شدت 45 تا 50 درصد VO2max) آغاز شد و در هفته دوازدهم به ۴۸ دقیقه رسید. برنامه تمرینی در گروه MICT با ۲۰ دقیقه (با شدت 65 تا 70 درصد VO2max) آغاز و در هفته دوازدهم به ۴۸ دقیقه رسید. مقادیر بیان پروتئین­های PGC-1α و Tfam عضله دوقلو به روش وسترن بلات سنجیده شد. از روش آماری تحلیل واریانس-یک راهه برای تجزیه تحلیل داده­ها استفاده شد. بیان پروتئین PGC-1α در دو گروه HIIT (001/0 > p) و MICT (01/0 > p) در مقایسه با گروه کنترل افزایش معناداری داشت. همچنین، مقادیر پروتئین PGC-1α در گروه HIIT در مقایسه با گروه MICT افزایش معناداری داشت (01/0 > p). بیان پروتئین Tfam  در گروه HIIT (01/0 > p) و MICT (05/0 > p) در مقایسه با گروه کنترل افزایش معناداری داشت.  HIIT در مقایسه با MICT به افزایش بیشتر بیان پروتئین­های PGC-1α و Tfam در عضله دوقلوی رت­های سالمند شده منجر است.

کلیدواژه‌ها


عنوان مقاله [English]

The Comparison of the Influence of 12-Week High- Intensity Interval Training and Continuous Moderate Intensity Training on PGC-1α and Tfam Mitochondrial Proteins Expressions in Gastrocnemius Muscle of Elderly Rats

نویسندگان [English]

  • A. Bakhtiyari 1
  • A. Gaeni 1
  • S. Choobineh 1
  • M.R. Kordi 1
  • M. Hedayati 2
1 Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
2 Prevention and Treatment of Obesity Research center, Research Institute for Endocrine Science, Shahid Beheshti University of Medical Sciences and health services, Tehran, Iran
چکیده [English]

Endurance training increase the proteins, enzymes and mitochondrial functions in human and animal samples. The purpose of this study was comparison of the influence of 12-week high-intensity interval training (HIIT) and moderate intensity continuous training (MICT) on PGC-1a and Tfam mitochondrial proteins expression in gastrocnemius muscle of elderly rats. In this study 45 elderly 22 months old Wistar rats (weighing 325-375g) were randomly divided into three groups: HIIT, MICT, and the control group. The exercise in the HIIT group was started with 20 minutes (2 minutes at 85-90% VO2max intensity and 2 minutes of recovery period at 45-50% VO2max intensity) in the first week and then reached to 48 minutes in the 12th week. The training program in the MICT group was begun with 20 minutes (at 65-70% VO2max intensity) and reached to 48 minutes in the 12th week. The expression levels of PGC-1α and Tfam proteins in gastrocnemius muscles were measured by Western blot method. One-way ANOVA was used to analyze the data. The PGC-1α expression was significantly increased in the HIIT (P< 0.001) and MICT (P<0.01) groups compared to the control group. Also, PGC-1α levels were significantly increased in the HIIT group compared to the MICT group (P<0.05). Tfam expression was significantly increased in the HIIT (P<0.01) and MICT (P<0.05) groups compared to the control group. HIIT resulted in a greater increase in PGC-1α and Tfam expressions in the gastrocnemius muscle of elderly rats compared to MICT.

کلیدواژه‌ها [English]

  • Mitochondrial biogenesis
  • Aging
  • High-intensity interval training
  • Moderate continuous training
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