اثر شش هفته تمرین هوازی و عصاره دارچین بر بیان ژن اینترلوکین یک بتا در بافت ماهیچه‌ی موش های نر تغذیه شده با رژیم غذایی پرچرب

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

چکیده

در افراد چاق، سطح بیان اینترلوکین یک بتا بالاتر است، به طوری که میزان بیان اینترلوکین یک بتا با سطح شاخص توده بدنی و توده چربی ارتباط مثبت مستقیمی دارد. پژوهش حاضر با هدف تعیین اثرات اجرای شش هفته تمرین هوازی، مصرف عصاره دارچین و غذای پرچرب بر بیان ژن اینترلوکین یک بتا در بافت ماهیچه موش­های نر تغذیه شده با رژیم غذایی پرچرب با استفاده از موش‌های صحرایی نر دو ماهه‌ نژاد ویستار انجام شد تعداد 50 سر موش صحرایی نر دو ماهه پس از دو هفته نگهداری در شرایط کنترل شده به پنج گروه کنترل, رژیم پرچرب، عصاره دارچین و رژیم پرچرب، تمرین هوازی و رژیم پرچرب و تمرین هوازی و عصاره دارچین و رژیم پرچرب تقسیم شدند. عصاره دارچین به میزان 200 میلی­گرم به ازائ هر کیلوگرم از وزن بدن و امولسیون غذای پرچرب مازاد بر آب و غذای معمولی جوندگان روزانه به مقدار 5/1 میلی­گرم به ازاء هر کیلوگرم از وزن بدن به مدت شش هفته به صورت گاواژ داده شد. گروه تمرین برای پنج روز در هفته و به مدت شش هفته در برنامه‌ تمرین هوازی روی نوارگردان شرکت کردند. میانگین متغیرهای پژوهش در بین گروه­ها (بجز گروه کنترل) با هدف تعیین تفاوت­های بین گروهی، با آزمون­های آنالیز واریانس یک طرفه و بونفرونی مقایسه شدند. میانگین متغیرهای پژوهش در بین هر گروه با گروه کنترل با هدف تعیین تفاوت­های درون گروهی، با آزمون آماری تی تک نمونه در سطح معنی­داری 05/0 ≥ p  مقایسه شدند. تفاوت متغیر وزن بدن در پس آزمون در بین تمامی گروه­ها معنی­دار بود. تفاوت میانگین­های متغیر اینترلوکین یک بتا در بافت ماهیچه موش­های نر تغذیه شده با رژیم غذایی پرچرب پس از شش هفته تمرین هوازی، مصرف عصاره دارچین و غذای پرچرب معنی­دار بود.

کلیدواژه‌ها


عنوان مقاله [English]

Protective Effect of Royal Jelly on Methotrexate Infertility in Female Mice

نویسندگان [English]

  • Hossein Bakhoda
  • Nematollah Nemati
  • Tahereh Bagherpoor
Department of Physical Education and Sport Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran
چکیده [English]

Methotrexate is a chemotherapy drug that is widely used in neoplastic disorders and ectopic pregnancy. In previous research, the toxicity of methotrexate has been reported on the genital tract. The properties of Royal jelly such as impotence improvement, and antioxidant and anticancer function have been reported. Therefore, in the present research, the effect of Royal jelly on ovarian cells in NMRI female mice treated with methotrexate was investigated. In this study, 70 female mice were divided into: negative control (without injection), positive control (received 0.5 mg/kg methotrexate), sham, and three experimental groups (T1, T2, and T3 received methotrexate plus 25, 50 and 100 mg/kg Royal jelly). Intraperitoneal injection was performed for 8 days. After histological processing and staining, the ovarian tissue, primary, secondary and graafian follicles, yellow body, as well the number of generation was investigated. Data were analyzed by Tukey test and one way ANOVA. Results showed there was a significant decrease in the number of generation in positive control, but the number of generation in experimental groups returned to the initial state. Regarding the duration of fertility, there was a significant decrease in the fertility rate in T1 and T3 compared to positive control group. The results of estrogen hormone showed a significant decrease in positive control group compared to negative control and increased significantly in T1 and T2. The number of primary, secondary, and graafian follicles in T 2 and T3 showed a significant increase (P<0.001). According to the present study, it was found that with further investigations, the appropriate dose of Royal jelly could act as a protective agent against the side effects of methotrexate and improve fertility.

کلیدواژه‌ها [English]

  • Methotrexate
  • royal jelly
  • Ovary
  • NMRI mouse
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