بررسی تاثیر دیازینون بر تغییرات هیستومورفومتریک هیپوکامپ جنین موش کوچک آزمایشگاهی نژاد Balb/c

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

دیازینون دسته‌ای از ارگانوفسفره­های آفت­کش است که بطور وسیع در کشاورزی استفاده می شود. این ماده سمی از طریق تنفسی، پوستی و گوارشی جذب می­شود و بر بافت های مختلف تاثیر می­گذارد. بنابراین هدف از این تحقیق بررسی اثرات دیازینون بر هیستومورفومتری هیپوکامپ جنین موش کوچک آزمایشگاهی نژاد Balb/c می­باشد. تعداد 25 سر موش کوچک آزمایشگاهی ماده بالغ نژاد c/Balb به 5 گروه 5 تایی تقسیم شدند.گروه شاهد دیازینونی دریافت نکرد. گروه­های شم الف و ب، امولسی فایر را به ترتیب به مقدار 52/0 و 2/5 میکرولیتر در واحد حجم (5000 سانتی متر مکعب در دسیکاتور) و گروه‌های تجربی الف و ب، دیازینون را به ترتیب به مقدار 3/1 و 13 میکرولیتر در واحد حجم، از روز 7 تا 18 بارداری، یک روز در میان بصورت استنشاقی دریافت کردند. در پایان روز 18 بارداری موش­ها کشته شده، جنین­ها از بدن حیوان خارج گردیده، از نظر ظاهری بررسی شدند. پس از فیکس شدن و پردازش جنین­ها، مقاطع 5 میکرونی تحت رنگ­آمیزی هماتوکسیلین-ائوزین قرار گرفت و بررسی هیستومورفومتری از هیپوکامپ انجام شد. میانگین ضخامت لایه‌‌های مختلف CA1، CA2 و CA3  هیپوکامپ در گروه‌های تجربی الف و ب کاهش داشت. شمارش میانگین تعداد سلول­ها در لایه‌‌های مختلف هیپوکامپ و شکنج دندانه‌ای در گروه تجربی الف و ب افزایش نشان داد. به نظر می­رسد که استنشاق دیازینون در مقادیر بالا (دوز 13 میکرولیتر در واحد حجم) در موش باردار می­تواند سبب تغییرات هیستومورفومتریک در روند تکامل هیپوکامپ گردد.

کلیدواژه‌ها


عنوان مقاله [English]

The Study of the Effects of Diazinon on Histomorphometrical Changes of Hippocampus in the Balb/c Mouse Embryo

نویسندگان [English]

  • Mahsa Momayez Haghighi
  • Shiva Nasiraei moghadam
  • Mehrangiz Sadoughi
Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Diazinon is an organophosphorus pesticide that is broadly used in agriculture. This toxic material is absorbed via inhalation, skin contact, or digestion and affects different tissues. The goal of this study was therefore to evaluate the diazinon effect on histomorphometrical changes of hippocampus in the small laboratory Balb/c mouse embryo. Twenty-five adult laboratory female Balb/c mice were equally divided into five groups of 5. The control group received no diazinon. The sham A and B groups received emulsifier at doses of 0/52 and 5.2 microliter in volume unit (5000cm3 in desiccator), respectively, and the experimental groups A and B received inhaled diazinon at doses of 1/3 and 13 microliter in the volume unit, respectively from day 7 until 18 of gestation every other day. The mice were killed on gestation day 18 and the embryos were removed from the animal's body and examined in terms of the appearance. After the embryos were fixed and processed, the 5-micrometer sections were stained using hematoxylin and eosin (H&E) technique and the histomorphometric changes of the hippocampus were investigated. The mean thickness of various hippocampus layers of CA1, CA2, and CA3 decreased in experimental groups A and B. The mean number of cells count in different layers of hippocampus and dentate gyrus increased in experimental groups A and B. Diazinon inhalation at high doses (13 microliters in volume unit) seems to be able to cause histological changes in the evolution process of hippocampus in pregnant mice.

کلیدواژه‌ها [English]

  • Embryo
  • Hippocampus
  • Mice
  • diazinon
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