مطالعه مولکولی گونه‌های بابزیا (Babesia) در گاوهای استان مازندران

نوع مقاله: مقاله پژوهشی

نویسندگان

بخش تحقیقات بیماری‌های انگلی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

گونه‌های مختلف بابزیا (Babesia) از شاخه­ی اپی­کمپلکسا، انگل داخل گلبول‌های قرمز می‌باشند که طیف وسیعی از حیوانات اهلی و وحشی و همچنین انسان را آلوده می‌نمایند. بابزیوز یکی از بیماری‌های جدی در صنعت گاوداری می‌باشد که بیش از نیم میلیارد از گاوهای دنیا به آن مبتلا می‌باشند. هدف از این مطالعه تعیین گونه‌های بابزیا در گاوهای استان مازندران بود. در مجموع تعداد 210 نمونه خون از طریق رگ وداج گاو به‌طور تصادفی اخذ شد. در ابتدا DNA استخراجی از نمونه‌های خونی با جفت آغازگری که قطعه حدود 400 جفت بازی از ژن 18S rRNA جنس بابزیا را تکثیر می‌کرد، تکثیر شد. تمامی نمونه‌های مثبت گاوی با nested-PCR semi اختصاصی ازنظر وجود بابزیا بایجمینا و بابزیا بوویس بررسی شدند. نتایج نشان داد که شیوع آلودگی بابزیا بایجمینا و بابزیا بوویس گاوی به ترتیب 33/33% و 6/28% بود. آزمون مربع کای جهت مقایسه میزان شیوع نسبت به فصول سال، سن دام و نوع دامداری (سنتی– نیمه‌صنعتی) انجام شد. جهت تجزیه‌وتحلیل داده‌ها از نرم‌افزار SPSS استفاده و سطح معنی‌دار بودن 05/0 در نظر گرفته شد. نتایج این تحقیق نشان می‌دهد که بابزیوز از بیماری‌های مهم و قابل‌توجه در گاوهای استان مازندران بخصوص در فصول فعالیت کنه‌ها می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Molecular Study of Babesia Species in the Cattles of Mazandaran Province

نویسندگان [English]

  • Nasrollah Vahedi Nouri
  • Vahid Noaman
Animal Parasitic Disease Research Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Different Babesia species originating from the Apicomplexa family are the parasites existing in red blood cells, and they can widely affect the domestic and wild animal and also human. Babesiosis is a serious disease in cattle breeding industry that has affected more than 0.5 billion of the cattles in the world. This study has been performed aiming to determine the Babesia species in the cattles of Mazandaran province. A total number of 210 blood samples were randomly collected from the jugular vein of the cattles. In the beginning, the DNA extracted from the blood sampleswas replicated with a primer pair  replicating a 400-bp fragment of the Babesia 18S rRNA gene. All the positive bovine samples were examined with the specific semi nested-PCR for checking Babesia bigemina and Babesia bovis. The results showed that the overall prevalence rates of Babesia bigemina and Babesia bovis infection in the cattles were 33.33% and 28.6%, respectively. Chi-square test was used to compare the prevalence of the infetion in terms of seasons, the livestock age, and the type of livestock husbandry (traditional - semi-industrial). SPSS software was used for data analysis and the significance level was considered p < 0.05. The results of this study show that Babesiosis is one of the most important diseases in the cattles of Mazandaran province, especially in the seasons of the ticks activity.

کلیدواژه‌ها [English]

  • Babesia species
  • cattle
  • Molecular
  • Mazandaran
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