اثرات سطوح مختلف عصاره یونجه (Medicago sativa) بر عملکرد آنزیم های کبدی و پارامترهای سرمی بچه‌ ماهیان قزل آلای رنگین‌ کمان (Oncorhynchus mykiss

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر، ایران

چکیده

این پژوهش به منظور بررسی اثرات عصاره یونجه (Medicago sativa) بر عملکرد آنزیم­های کبدی و پارامترهای سرم خون بچه ماهیان قزل­آلای رنگین­کمان (Oncorhynchus mykiss) انجام شد. در این راستا تعداد 750 قطعه بچه­ماهی با میانگین وزن 5/0 ± 4/10 گرم در استخرهای بتنی پرورشی در 5 تیمار غذایی به ترتیب شامل، صفر (شاهد)، 5/0، 1، 2 و 3 درصد عصاره در جیره با سه تکرار به مدت 60 روز تغذیه شدند. بررسی فراسنجه­های سرمی نشان داد، بین ماهیان تغذیه شده با عصاره یونجه و گروه شاهد تفاوت معنی­داری وجود داشت. بالاترین و کمترین سطح گلوکز سرم به ترتیب در تیمار 4 و تیمار 1 (شاهد) مشاهده گردید (05/0p < ). بالاترین سطح پروتئین تام سرم در تیمار 2 و کمترین آن در تیمار 5، مشاهده گردید (05/0p < )، هر چندکه بین تیمار شاهد با تیمار 2 و تیمار 4، از نظر سطح پروتئین تام سرم، اختلاف معنی­داری مشاهده نگردید (05/0p > ). بالاترین سطح آلبومین سرم در تیمار شاهد، ثبت گردید. بر اساس نتایج حاصله، سطوح مختلف عصاره یونجه باعث یک روند کاهشی در میزان تری­گلیسرید و کلسترول سرم گردید. بیشترین سطح فسفر سرم در تیمار 4 و کمترین میزان فسفر سرم در تیمار شاهد، مشاهده گردید (05/0p < ). سطوح منیزیم سرم نیز تحت تاثیر عصاره یونجه قرار گرفت (05/0p < ). بر اساس نتایج حاصله، در سطوح سرم گلوتامیک اگزالواستیک ترانس­آمیناز SGOT و سرم گلوتامیک پیروویک ترانس آمیناز SGPT سرم، اختلاف معنی­داری بین تیمارهای آزمایشی، در طول دوره 60 روزه تغذیه­ای مشاهده نگردید (05/0p > ). بالاترین سطح آلکالین فسفاتاز (ALP) سرم در تیمار 5 و کمترین آن در تیمار شاهد، مشاهده گردید (05/0p < ). بالاترین سطح لاکتات دهیدروژناز (LDH) سرم در تیمار شاهد و کمترین آن در تیمار 3 و تیمار 4 مشاهده گردید (05/0p < ). بر اساس نتایج حاصله، سطح 2 درصد عصاره یونجه در جیره غذایی بهترین تاثیر را بر پارامترهای بیوشیمیایی سرم و آنزیم­های کبدی داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Different Levels of Alfalfa (Medicago sativa) Extract on Hepatic Enzymes Performance and Serum Parameters in Rainbow Trout (Oncorhynchus mykiss)

نویسندگان [English]

  • Ahmad Kolivand
  • Seyed Mohammad Mousavi
  • Mohammad zakeri
  • Vahid Yavari
  • Nasim Zanguee
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Iran
چکیده [English]

This study was conducted to investigate the effects of alfalfa (Medicago sativa) extract on hepatic enzymes performance and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). In this regard, 750 fish (initial weight: 10.4 ± 0.5 g) distributed in concrete pools and fed by 5 dietary treatments including 0 (control), 0.5, 1, 2, and 3 percent of alfalfa extract, respectively for 60 days. Serum parameters analysis showed that there was a significant difference between fish fed with alfalfa extract and control group. The highest and lowest levels of serum glucose were observed in treatment 4, and treatment 1 (control), respectively (p < 0.05). The highest level of total serum protein was observed in treatment 2, and the lowest was observed in treatment 5 (p < 0.05). However, there was no significant difference between control, treatments 2, and treatment 4 in terms of total serum protein level (p > 0.05). The highest level of serum albumin was recorded in control. Based on the results, different levels of alfalfa extract led to a downward trend in serum triglyceride and cholesterol levels. The highest level of serum phosphorus was observed in treatment 4, and the lowest level was observed in the control (p < 0.05). Serum magnesium levels were also affected by alfalfa extract (p < 0.05). Based on the results, no significant differences were observed between experimental treatments in serum SGOT and SGPT levels during the 60-day experimental feeding period (p > 0.05). The highest level of serum alkaline phosphatase (ALP) was in treatment 5 and the lowest level of serum ALP was observed in control (p < 0.05). The highest level of serum lactate dehydrogenase (LDH) was observed in control and the lowest in treatments 3 and 4 (p < 0.05). Based on the results, 2 percent of alfalfa extract in the diet had the best effects on serum biochemical parameters and hepatic enzymes.

کلیدواژه‌ها [English]

  • Rainbow trout
  • Alfalfa extract
  • Hepatic enzyme
  • serum biochemical parameters
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