بررسی اثر ماده مخدر متامفتامین بر رشد سلولی سلول های بنیادی مشتق شده از بافت آندومتر رحم موش های صحرایی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران

2 مرکز تحقیقات سوختگی و ترمیم زخم، دانشگاه علوم پزشکی شیراز، شیراز، ایران

چکیده

متامفتامین به‌عنوان یک ماده توهم‌زا است که مورد سو مصرف میلیون­ها نفر در سراسر دنیا قرار می­گیرد. ایجاد سمیت سلولی در برخی از رده­های سلولی از اثرات گزارش‌شده، این ماده روان‌گردان می‌باشد. لذا این مطالعه با هدف بررسی اثر متامفتامین بر رشد سلول‌های بنیادی مزانشیمی بالغ استخراج شده از بافت آندومتر رحم موش­های صحرایی انجام گردید. در این مطالعه تجربی سلول‌های بنیادی از بافت آندومتر رحم موش‌های صحرایی استخراج شد و پس از کشت آنها، با روش فلوسایتومتری و بواسطه مارکرهای CD34، CD90، CD105 و مزانشیمی بودن آنها مورد بررسی قرار گرفت. در پاساژ سوم کشت سلولی، اثر سمیت متامفتامین در غلظت 6/0 میلی‌مولار در طول مدت 1 الی 8 روز بر رشد سلول‌های بنیادی مزانشیمی مشتق شده از بافت آندومتر رحم توسط فرمول PDT= T×  محاسبه گردید سلول‌های جدا شده از بافت آندومتر رحم، 24 ساعت بعد از انتقال به فلاسک کشت سلولی، کاملاً به کف فلاسک چسبیدند. این سلول­ها از نظر بیان مارکرهای سطحی غیر هماتوپویتیک (CD90، CD105) مثبت و از نظر بیان مارکر هماتوپویتیک (CD34)  منفی بود، بنابراینهویت مزانشیمی بودن این سلول­ها تائید گردید. نتایج آزمون شمارش سلولی نیز حاکی از کاهش معنادار رشد سلول‌های تیمار شده با 6/0 میلی‌مولار متامفتامین در مقایسه با گروه شاهد بود. نتایج حاصل از این مطالعه نشان داد سلول‌های جدا شده از بافت آندومتر ، از نوع بنیادی مزانشیمی بوده که بر اساس نتایج حاصل از شمارش سلولی مشخص گردید که متامفتامین می‌تواند با القاء اثرات سمیت سلولی باعث مهار رشد در آنها گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Effects of Methamphetamine Drug on Cell Growth of Stem cells Derived from Uterine Endometrial Tissue of Rats

نویسندگان [English]

  • Zahra Goudarzi 1
  • Seyyed Ebrahim Hosseini 1
  • ,Davood Mehrabani 2
  • Sara Hashemi 2
1 Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2 Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده [English]

Methamphetamine is a hallucinogenic substance abused by millions of people all around the world. Cellular toxicity in some cell lines is one of the reported effects of this psychoactive substance. Therefore, this study was conducted to investigate the effect of methamphetamine on the growth of adult mesenchymal stem cells extracted from uterine endometrial tissue in rats. In this experimental study, stem cells were extracted from uterine endometrial tissue in rats, and after cultivation, they were investigated in terms of being mesenchymal using flucytometry method and by CD34, CD90 and CD105 markers. In the third passage of cell culture, the effect of methamphetamine toxicity at a concentration of 0.6 mmol was investigated in a 1-8 day period on the growth of mesenchymal stem cells derived from uterine endometrial tissue by formula PDT= T× . Cells isolated from uterine endometrial tissue adhered completely to the floor of the flask 24 hours after being transferred to the cell culture flask. These cells were positive for non-hematopoietic surface marker expression (CD90, CD105) and negative in terms of hematopoietic marker (CD34) expression, so the mesenchymal identity of these cells was confirmed. The results of the cell count test also showed a significant reduction in the growth of cells treated with 0.6 mmol methamphetamine compared to the control group. The results of this study showed that the cells isolated from the uterine endometrial tissue were of the basic mesenchymal type. The results of cell counting revealed that methamphetamine can inhibit growth by inducing the effects of cell toxicity.

کلیدواژه‌ها [English]

  • Methamphetamine
  • Endometrium
  • Stem cell
  • Cell growth
  • Rat
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