اثرات رفتاری استرس پیش از تولد بر زادگان بالغ رت‌های تحت استرس در دوران بارداری

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد تهران مرکز، تهران ،ایران

چکیده

استرس یک محرک قوی است که برای تعدادی از بیماری­های روانی و نوروبیولوژیک به عنوان یک فاکتور آسیب­زا تلقی می­شود. در‌میان دوره­های مختلف زندگی، دوره جنینی از حساسیت و انعطاف­پذیری بالایی برخوردار است و تجربیات مادر در دوران بارداری می­تواند به گونه­ای شکل­دهنده و پیش­بینی­کننده آینده رفتاری فرزند وی باشد.در مطالعه حاضر از مدل جدیدی ازاسترس اجتماعی در دوران بارداری مادر استفاده­شد و تاثیر آن در رفتار فرزندان در بزرگسالی مورد مطالعه قرار گرفت. از20 سر رت ویستار باوزن تقریبی150-200گرم استفاده شد.رت­ها پس از بارداری به دو گروه کنترل و استرس تقسیم شدند.گروه استرس از هفته دوم بارداری، تحت استرس بی­حرکتی و شکارگر همراه با بی­ثباتی اجتماعی قرار­گرفت. تاثیر استرس مادر، در فرزندان در سن بزرگسالی با آزمون جعبه تاریک-­روشن مورد بررسی قرار گرفت. در گروه استرس نشان داد که T1 (میزان تاخیر در اولین ورود به محفظه روشن) به­طور معناداری (0020/0=p < /em>) نسبت به گروه کنترل، افزایش داشت. T2 (زمان کلی که حیوان در محفظه روشن می‌گذراند) و n (تعداد ورود به محفظه روشن) به طور معنا­داری به­ترتیب (0003/0 = p < /em> و 0001/0<  p < /em>)  نسبت به گروه کنترل کاهش داشت. این تغییرات در مورد جنس نر برای هرکدام از پارامتر­های مذکور نسبت به جنس ماده بارزتر بود. القای مدل جدیدی از استرس اجتماعی تاثیر قابل­توجه و متفاوتی را در رفتار مادر و به دنبال آن دررفتار فرزندان وی درسن بزرگسالی داشت. همچنین نتایج نشان­دهنده تفاوت مقاومت دو جنس نر و ماده در برابر استرس بود.

کلیدواژه‌ها


عنوان مقاله [English]

The Behavioral Effects of Prenatal Stress on Adolescent Offspring of Rats under Pregnancy Stress

نویسندگان [English]

  • Elaheh Maherani
  • Nastaran Asghari Moghaddam
  • bahareh pakpour mahmoudabad
Department of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Stress is a strong stimulant that is considered as a damaging factor for a number of psychiatric and neurobiological disorders. Among different periods of life, embryonic life has a high degree of sensitivity and flexibility; hence, maternal experiences during pregnancy can form and predict the behavior of her descendent. In the present study, a new model of social stress was used during the rat pregnancy period to examine its influence over the behavior of adolescent offspring. Twenty Wistar rats (weighed approximately150-200gr) were used. After gestation, rats were divided into a control group and a stress group. The stress group members were subjected to restraint stress and predator stress with social instability since the second week of pregnancy. The effect of maternal stress was studied in offspring by dark-light box test during adolescence. The stress group showed a significant increase in the delay time for the first entry into the light box (T1) in comparison to the control group (p =0.0020(. The total time the animal remains in the light box (T2) and the number of the entries into the light box (n) decreased in comparison with control group, respectively )p =0.0003, p <0.0001). These variations were more prominent for males than females for all the above mentioned parameters. The induction of a new social stress model had a significant and different impact on the behavior of the mother and her offspring in adulthood. The results also revealed the difference between male and female resistance to stress.

کلیدواژه‌ها [English]

  • Prenatal stress
  • Rat
  • Predator stress
  • Restraint stress
  • Nervous system
  • offspring
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