اثر حفاظتی کورکومین بر میزان ترانس‌آمینازهای کبدی و آلکالین فسفاتاز در موش های صحرایی نر به دنبال دریافت نانوذرات نیکل

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، پردیس واحد علوم و تحقیقات فارس، دانشگاه آزاد اسلامی، فارس، ایران

2 گروه زیست شناسی،واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران

چکیده

نانوذرات نیکل به صورت گسترده در محیط زیست پراکنده است که مواجهه شدن با آن باعث آسیب به بافت­های مختلفی بدن از جمله شش، کبد، بیضه و کلیه می­شود، که تاثیرات عمده آن بر بافت کبد می باشد، بنابراین هدف از این مطالعه بررسی اثر حفاظتی کورکومین بر میزان آنزیم­های کبدی ALT، AST و ALP­ به دنبال دریافت نانوذرات نیکل در موش­های صحرایی نر می­باشد. در این مطالعه تجربی، 50 سر موش صحرایی نر نژاد ویستار به صورت تصادفی در 5 گروه (کنترل، شاهد، گروه دریافت­کننده (50 میلی­گرم بر کیلوگرم نیکل)، گروه دریافت­کننده (50 میلی­گرم بر کیلوگرم نیکل+ 150 میلی­گرم بر کیلوگرم کورکومین) و گروه دریافت کننده (50 میلی­گرم بر کیلوگرم نیکل+ 300 میلی­گرم بر کیلوگرم کورکومین) قرار گرفتند. تیمار موش­های صحرایی به مدت 28 روز ادامه داشت. در پایان پس از خونگیری از موش­ها، آنزیم­های (ALT، AST و ALP)­ بررسی شد. نانوذره نیکل ﺑﺎﻋﺚ اﯾﺠﺎد ﺗﻔﺎوت ﻣﻌﻨﯽ دار در ﻣﯿﺰان ﺳﺮﻣﯽ آﻧﺰﯾﻢﻫﺎی آﺳﭙﺎرﺗﺎت آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز، آﻻﻧﯿﻦ آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز و آﻟﮑﺎﻟﯿﻦ ﻓﺴﻔﺎﺗﺎز در سطح 0001/0>  p < /em> ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل شد. در حالی که کورکومین در دوزﻫﺎی ﻣﺨﺘﻠﻒ ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﻣﻌﻨﯽدار ﺳﻄﺢ ﺳﺮﻣﯽ آﻧﺰﯾﻢﻫﺎی ﺑﯿﺎن ﺷﺪه در سطح 0001/0>p < /em>  ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل گردید. تیمار با ﮐﻮرﮐﻮﻣﯿﻦ موجب تخفیف آسیب کبدی القا شده با نانوذره نیکل می­شود. همچنین می­توان گفت ﻣﺼﺮف ﻫﻤﺰﻣﺎن ﮐﻮرﮐﻮﻣﯿﻦ ﺑﺎ نانوذره نیکل ﺑﻪ دﻟﯿﻞ ﺧﻮاص آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﮐﻮرﮐﻮﻣﯿﻦ، ﺑﺎﻋﺚ ﮐﺎﻫﺶ این ﻓﻌﺎﻟﯿﺖ می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Protective Effect of Curcumin on Hepatic Enzyme Transaminases and Alkaline Phosphatase in Male Rats After Receiving Nickel Nanoparticles

نویسندگان [English]

  • Rahimeh Khezri Motlagh 1 2
  • Akbar Vahdati 2
  • Seyed Ebrahim Hosseini 2
  • Mohammad Amin Edalatmanesh 2
1 Department of Biology,Fars Science and Research branch,Islamic Azad University,Fars,Iran
2 Department of Biology,Fars Science and Research branch,Islamic Azad University,Fars,Iran
چکیده [English]

Nickel nanoparticles are widely distributed in the environment, resulting in damage to various tissues of body, including the lung, liver, testis, and kidney which is the major effect on the liver tissue. Therefore, this study aims to investigate Curcumin protective effect on the number of enzymes (ALT, AST, and ALP) to obtain nickel nanoparticles in rats. In this experimental study, 50 Wistar adult male rats were randomly selected and divided in to five groups (control, witness, receiver group (nickel 50 mg/kg), receiver group (nickel 50 mg/kg + Curcumin 150 mg/kg), and receiver group (nickel 50 mg/kg + Curcumin 300 mg/kg). The treatment of rats continued for 28 days. In the end, after bloodletting of rats, (ALT, AST and ALP) enzymes were investigated. Nickel nanoparticles caused a significant difference in Serum level of Aspartate Aminotransferase, Alanine aminotransferase, and Alkaline Phosphatase enzymes in the class of p < /em> ˂ 0.0001 than the control group. While, Curcumin in different dosages caused a significant reduction in the serum level of enzymes expressed at the class of p < /em> ˂ 0.0001 ratio to the control group. The treatment by Curcumin results in reducing the liver damage induced by nickel nanoparticles. Also, we can say that the concurrent consumption of Curcumin with nickel nanoparticle due to the antioxidant properties of Curcumin reduces this activity

کلیدواژه‌ها [English]

  • Curcumin
  • nickel nanoparticles
  • Aspartate aminotransferase
  • Alanine amino transferase
  • alkaline phosphatase
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