تاثیر نانوذرات نقره بر زیست پذیری رده سلولی فیبروبلاست ریه (MRC-5)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه مهندسی محیط زیست و صنایع غذایی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

نانو‌نانو­ذرات نقره (AgNPs) با دارا بودن خواص ضد­میکروبی و ضد­تکثیری بسیار قوی، کاربرد زیادی در علوم پزشکی دارند، با وجود این، اطلاعات اندکی در رابطه با تاثیر آن­ها بر سلول­های سالم وجود دارد و مواردی مبنی بر سمیت آن­ها گزارش شده­است. این تحقیق با هدف بررسی تاثیر نانوذرات ­نقره بر درصد زیست پذیری رده سلولی MRC-5 انجام­گرفت. در این مطالعه تجربی، در طی24 ،48 و 72­ساعت، غلظت­های مختلف نانو­ذرات ­نقره (56/1، 125/3، 25/6 ،5/12­، 25­، 50­ و 100 میکروگرم بر میلی­لیتر) بر رده سلولیMRC-5  با روشMTT  بررسی شد. داد­ه­های حاصل، با استفاده از نرم­افزار SPSS، آزمون آماری ­anova یک طرفه، تست توکی ارزیابی شد. تیمار سلول­های نرمالMRC-5  با غلظت­های مختلف نانو­ذرات­ نقره بعداز 24­، 48 و 72­ ساعت با روشMTT ، کاهش معنی­دار بقاء سلول­ها را در غلظت­های 25، 50 و 100­ میکروگرم برمیلی لیتر را نشان داد (05/0 > p < /em>) نتایج این بررسی نشان داد که نانو­ذرات­ نقره  بر سلول­های نرمال MRC-5 به صورت وابسته به دوز اثر مهاری دارد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Silver Nanoparticles on the Viability of Lung Fibroblast Cell Line (MRC-5)

نویسندگان [English]

  • Yasamin Dastgir 1
  • Zahra Keshtmand 1
  • Katayoun Borhani 2
1 Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Environment and Food Industry Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Silver nanoparticles (AgNPs) with very strong antimicrobial and anti-proliferative properties are widely used in medical sciences. However, little is known about their effect on healthy cells, and there were the reports of their toxicity. This study aimed to investigate the effect of silver nanoparticles on the viability rate of the MRC-5 cell line. In this experimental study, during 24, 48, and 72 hours, different concentrations of silver nanoparticles (1.56, 3.125, 6.5, 12.5, 25, 50, and 100 μg/ml) per category MRC-5 cells were examined by MTT assay. Data were analysed using SPSS software, one-way ANOVA, Tukey test, and a significant level of p < /em> < 0.05. The treatment of normal MRC-5 cells with different concentrations of silver nanoparticles after 24, 48, and 72 hours by MTT method showed a significant reduction in the viability of cells at concentrations of 25, 50, and 100 μg/ml (p < /em> < 0.05). The results of this study showed that silver nanoparticles have a dose-dependent inhibitory effect on normal MRC-5 cells.

کلیدواژه‌ها [English]

  • silver nanoparticles
  • viability
  • MTT
  • MRC-5
1. Basavaraja S., Balaji S.D., Lagashetty A., Rajasab A.H., Venkataraman A., 2008. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Materials Research Bulletin Journal, 43(5):1164-1170.
2. Faraha M.A., Alib M.A., Chen S.M., Li Y., Hemaid F.M., Aboutarboush F.M., 2016. Silver nanoparticles synthesized fromAdenium obesum leaf extract induced DNA damage apoptosis and autophagy viageneration of reactive oxygen species. Colloids and Surfacce B: Biointerfaces, 141:158-169.
3. Fock K.M.,2014. Review article: the epidemiology and prevention ofgastric cancer. Alimentary Pharmacology and Therapeutics,40(3):250-260.
4. Kim S., Choi J.E., Choi J,Chung K.H., Park K, Yij J., et al.,2009. Oxidative Stress-Dependent Toxicity of Silver NanoparticlesIn Human Hepatoma Cells. Journal of Toxicology In vitro, 23(6):1076-1084.
6. Liesje S., Bart D., Paul V., Benny F., Pyck G. 2011.The Antibacterial Activity of Biogenic Silver and Its Mode of Action. World Journal of Microbiology and Biotechnology ,31(2):113-189.
7. Iiizumi M., Liu W., Pai S.K., Furuta E., Watabe K., 2008.Drug development against metastasisrelated genes and their pathways: a rationale for cancer therapy. Biochimica et Biophysica Acta,1786(2): 87-104.
8. Liu J., Sonshine D.A., Shervani S., Hurt R.H. 2010. Controlled release of biologically active silver from nanosilver surfaces. Journal of ACS Nano, 4(11): 6903-6913.
9. Mao B.H.,Tsai J.C., Chen C.W., Yan S.J., Wang Y.J., 2016.Mechanisms of silver nanoparticle-induced toxicity and important role of autophagy.­ Nanotoxicology,10(8): 1021-1040.
10. Mao HY., Laurent S., Chen W., Akhavan O., Imani M., Ashkarran AA., et al.,2013. promises, facts, opportunities, and challenges in nanomedicine. Chemical Review,113(5): 3407-3424.
11. Narayanan K.B., Sakthivel N.,2011. Green synthesis of biogenic metal nanoparticles byterrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatibleagents. Journal of Colloid and Interface Science, 169(2):59-79.
12. Patel V., Berthold D., Puranik, P., Gantar M., (2015). Screening of cyanobacteria and microalgae for their ability to synthesize silver nanoparticles with antibacterial activity. Biotechnology Reports, 5:112-119.
13. Rajeshkumar C., Malarkodi K., Paulkumar M., Vanaja G., Gnanajobitha G., Annadurai G. 2014. Algae mediated green fabrication of silver nanoparticles and examination of its ainst clinical pathogens. International Journal of Metals, 692643: 1-9.
14. Rashmezad M.A., Ali Asgary E., Tafvizi F., Shandiz S.A., Mirzaie A., 2015. Comparative study on cytotoxicity effect of biological and commercial synthesized nanosilver on human gastric carcinoma and normal lung fibroblast cell lines. Journal of Tehran University of Medical Sciences,72 (12):799-807.[In Persian]
15. Ravindran A., Chandran P., Khan SS.,2013. Biofunctionalized silver nanoparticles: advances and prospects. Colloids Surf B Biointerfaces, 105:342-352.
16. Salehzadeh A., Sadatshandiz A., Sadatnaeemi A,2018. Cytotoxicity Effect of Biosynthesized Silver Nanoparticles Using Macro Algae Laurencia caspica Extract against Breast Cancer T47D Cell Line. Scientific Journal of Ilam University of Medical Sciences, 26(1):52-61.[In Persian]
17. Saraniya Devi J., Valentin Bhimba B.,2012. Anticancer activity of silver nanoparticles synthesized by the seaweed Ulva lactuca in vitro. Open Access Scientific Reports,1(4):1-5.
18. Vankayala R., Hwang K.C.,2018. Near-Infrared-Light-Activatable Nanomaterial-Mediated Phototheranostic Nanomedicines: An Emerging Paradigm for Cancer Treatment. Advance Material, 30(23): e1706320.
19. Vijayaraghavan K., Kamala Nalini SP., Udaya Prakash N., Madhankumar D,2012. Biomimetic synthesis of silver nanoparticles by aqueous extract of Syzygium aromaticum. Material Letters,75:33-35.
20. Vieira AP., Stein EM., Andreguetti DX., Colepicolo P., Ferreira AMC.,2016. Preparation of silver nanoparticles using aqueous extracts of the red algae Laurencia oldingensis and Laurenciella sp. and their cytotoxic activities. Journal of Applied Phycology, 28(4): 2615-2622
21. Zhang XF, Liu ZG, Shen W.,Gurunathan S. 2016. Silver anoparticle synthesis, characterization, properties, applications and therapeutic approaches. International Journal of Molecular Science, 17(9):1534.
22. Zhu B., Li Y., Lin Zh., Zhao M.,Xu T.,Wang Ch. 2016. Silver nanoparticle induce HePG2 cells apoptosis through ROS-mediated signaling pathway.  Nanoscale Research Letters, 11:198-206.