اثر شش هفته تمرین هوازی و عصاره دارچین بر بیان ژن اینترلوکین شش در بافت چربی موش های نر تغذیه شده با رژیم غذایی پرچرب

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

چکیده

پژوهش تجریی حاضر با هدف تعیین اثرات اجرای شش هفته تمرین هوازی، مصرف عصاره دارچین و غذای پرچرب بر بیان ژن اینترلوکین شش در بافت چربی موش­های نر تغذیه شده با رژیم غذایی پرچرب با استفاده از یک مدل حیوانی (موش‌های صحرایی نر دو ماهه‌ نژاد ویستار 14848) در قالب یک طرح پژوهشی چند گروهی با گروه کنترل انجام شد. متغیرهای مستقل این پژوهش شامل اجرای شش هفته تمرین هوازی، مصرف شش هفته عصاره دارچین و شش هفته رژیم غذایی پرچرب بودکه به عنوان متغیر زمینه ای منظور می­شود. متغیر وابسته نیز شامل میزان بیان ژن اینترلوکین شش در بافت چربی بود. تعداد 50 سر موش پس از دو هفته نگهداری در شرایط کنترل شده به طور تصادفی به پنج گروه شامل گروه های کنترل، رژیم پرچرب، عصاره دارچین و رژیم پرچرب، تمرین هوازی و رژیم پرچرب و گروه تمرین هوازی و عصاره دارچین و رژیم پرچرب تقسیم شدند. همه گروه­ها به آب و غذای معمولی جوندگان به صورت آزادانه دسترسی داشتند. به گروه­های دریافت کننده غذای پرچرب، روزانه به مقدار 5/1 میلی­گرم به ازاء هر کیلوگرم از وزن بدن به مدت شش هفته از امولسیون غذای پرچرب به صورت گاواژ داده شد. عصاره دارچین به میزان 200 میلی­گرم به ازای هر کیلوگرم از وزن بدن گاواژ شد. شیب نوارگردان در سرتاسر دوره‌ تمرین، 15 درصد بود. در صورت وجود تفاوت معنی­دار در نتایج این آزمون، از آزمون تکمیلی بونفرونی جهت تعیین دقیق تفاوت­های بین گروهی استفاده شد. میانگین متغیرهای پژوهش در بین هر گروه با گروه کنترل با هدف تعیین تفاوت­های درون گروهی، با استفاده از آزمون آماری تی تک نمونه مقایسه شد. سطح معنی­داری در کلیه آزمون ها 05/0 p < /em> <  بود.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Six Weeks Aerobic Training and Cinnamon Extract on IL-6 Gene Expression in Fat Tissue of Obese Male Feed by High Fat Diet

نویسندگان [English]

  • Amin Rigi
  • Tahereh Bagherpoor
  • Nematollah Nemati
Department of Sport Sience, Damghan Branch, Islamic Azad University, Damghan, Iran
چکیده [English]

Comparing the concurrent training order on plasma IL-6 levels in elderly male.
Introduction: This study aimed to investigate the comparison of a different orders of concurrent training on plasma IL-6 levels, insulin resistance index (HOMA) and
anthropometric characteristics of an elderly male. Forty women with 55-70-year-old which having no particular illnesses were divided into 4 groups: endurance-resistance, (n = 9), resistance-endurance (n = 10), interval resistance-endurance (n = 12) training, and control group (n = 9) randomly. Height, weight, BMI, WHR, and serum levels of myonectine glucose and insulin were measured and recorded before and after performing protocol. Experimental groups participated in concurrent training for 8 weeks, 3 sessions every week, 60 minutes in every session. A univariate (covariance) test was used for analyzing the data. A significant increase of vaspin (p < /em> = 0.009) and the significant decrease of glucose (p < /em> = 0.001) and HOMA index (p < /em> = 0.028) was observed in the experimental groups compared to control, no significant difference among experimental groups. There was not any significant difference among groups in insulin levels (p < /em> = 0.37). Older males can take advantage of the concurrent training with any orders of them.
 

کلیدواژه‌ها [English]

  • Sports
  • Cinnamom
  • Obese
  • IL-6
  • High fat diet

مراجع

  1. Badalzadeh, R., Shaghaghi, M., Mohammadi, M., Dehghan, G., Mohammadi, Z. 2014. The effect of cinnamon extract and long-term aerobic training on heart function, biochemical alterations and lipid profile following exhaustive exercise in male rats. Coronary Artery Disease, 2014: 2-8.
  2. Bray, G.A. 2003. Contemporary diagnosis and management of obesity: Handbooks in Health Care.
  3. Carey A.L., Steinberg, G.R., Macaulay, S.L., Thomas, W.G., Holmes, A.G., Ramm, G., 2006. Interleukin-6 increases insulin-stimulated glucose disposal in humans and glucose uptake and fatty acid oxidation in vitro via AMP-activated protein kinase. Diabetes, 55(10): 2688-2697.
  4. Chao, L.K., Hua, K.F., Hsu, H.Y., Cheng, S.S., Liu, J.Y., Chang, S.T. 2005. Study on the anti-inflammatory activity of essential oil from leaves of Cinnamomum osmophloeum. Journal of Agricultural and Food Chemistry, 53(18):7274-7278.
  5. Church, T.S., Thomas, D.M., Tudor-Locke, C., Katzmarzyk, P.T., Earnest, C.P., Rodarte, R.Q., 2011. Trends over 5 decades in US occupation-related physical activity and their association with obesity. PloS One, 6(5): e19657.
  6. Da Cunha Nascimento, D., de Sousa, N.M.F., de Sousa Neto, I.V., Tibana, R.A., de Souza, V.C., Vieira, D.C.L., 2015. Classification of pro-inflammatory status for interleukin-6 affects relative muscle strength in obese elderly women. Aging Clinical and Experimental Research, 27(6): 791-797.
  7. Ebrahimi, M., Khenar Sanami, S. 2015. Effects of high fat diet and high intensity aerobic training on interleukin 6 plasma levels in rats. Modares Journal of Medical Sciences: Pathobiology, 18(3): 110-116.
  8. Eckel, R.H., Grundy, S.M., Zimmet, P.Z., 2005. The metabolic syndrome. The Lancet, 365(9468):1415-1428.
  9. Eftekhari, E., Zafari, A., Gholami, M., 2016. Physical activity, lipid profiles and leptin. The Journal of Sports Medicine and Physical Fitness, 56(4): 465-469.
  10. Fairburn, C.G., Walsh, B.T., 2002. Atypical eating disorders (eating disorder not otherwise specified). Eating Disorders and Obesity: A Comprehensive Handbook, 2: 171-177.
  11. Flegal, K.M., Graubard, B.I. 2009. Estimates of excess deaths associated with body mass index and other anthropometric variables. The American Journal of Clinical Nutrition, 89(4): 1213-1219.
  12. Gadina, M., Hilton, D., Johnston, J.A., Morinobu, A., Lighvani, A., Zhou, Y-J., 2001. signaling by type I and II cytokine receptors: ten years after. Current Opinion in Immunology, 13(3): 363-373.
  13. Hasani-Ranjbar, S., Nayebi, N., Larijani, B., Abdollahi, M. 2009. A systematic review of the efficacy and safety herbal medicine used in the treatment of obesity. World Journal of Gastroenterology, 15(25): 3073.
  14. Kumanogoh, A., Ogata, M. 2010. The study of cytokines by Japanese researchers: a historical perspective. International Immunology, 22(5): 341-345.
  15. Liuzzi, J.P., Lichten, L.A., Rivera, S., Blanchard, R.K., Aydemir, T.B., Knutson, M.D., 2005. Interleukin-6 regulates the zinc transporter Zip14 in liver and contributes to the hypozincemia of the acute-phase response. Proceeding of the National Academy of Sciences of the United State of America, 102(19): 6843-6848.
  16. Loos, R.J., 2012. Genetic determinant of common obesity and their value in prediction. Best Practice and Research Clinical Endocrinology and Metabolism, 26(2):211-226.  
  17. Lukaszewicz, M., Mroczko, B., Szmitkowski, M. 2007. Clinical significance of interleukin-6 (IL-6) as a prognostic factor of cancer disease. Polish Archives of Internal Medicine, 117(5-6): 247-251.
  18. Mathew, S., Abraham, T.E. 2006. Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extract, through various in vitro models. Food Chemistry, 94(4): 520-528.
  19. Mohammadifar, Sh. 2010. The origin, history and trade routes cinnamon. History of Science, 9: 37-51.  
  20. Mojtaba, E., Sohaily, S., Parsian, H., Zand, A. 2011. Pro-inflammatory cytokine Interleukin-1 beta is associated with cardiovascular fitness in sedentary diabetic patients. Journal of Biodiversity and Environmental Sciences, 1(2): 37-44.
  21. Mollazadeh, H., Hosseinzadeh, H. 2016. Cinnamon effects on metabolic syndrome: a review based on its mechanisms. Iranian Journal of Basic and Medical Sciences, 19: 1258-1270.
  22. Na, Y.K., Hong, H.S., Lee, W.K., Kim, D.S. 2015. Increased methylation of interleukin 6 gene is associated with obesity in Korean women. Molecular and Cells, 38(5): 452-456.
  23. Nasery, L., Zafari, A., Banaeifar, A. A., 2015. The response of leptin and lipid parameters related to an aerobic exercise among young athlete and non-athlete womwn. Indian Journal of Fundamental and Applied Life Sciences, 5(S3): 788-798.
  24. Nemeth, E., Rivera, S., Gabayan, V., Keller, C., Taudorf, S., Pederson, B.K. 2004. IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. The Journal of Clinical Investigation, 113(9): 1271-1276.
  25. Nguyen, N.T., Magno, C.P., Lane, K.t., Hinojosa, M.W., Lane, J.S. 2008. Association of hypertension, diabetes, dyslipidemia, and metabolic syndrome with obesity: finding from the National Health and Nutrition Examination Survey, 1999 to 2004. Journal of the American College of Surgeon, 207(6): 928-934.
  26. Ossanloo, P., Najar, L., Zafari, A. 2012. The Effects of Combined Training (Aerobic Dance, Step Exercise and Resistance Training) on Body Fat Percent and Lipid Profiles in Sedentary Females of AL_ZAHRA University, European Journal of Experimental Biology, 2(5):1598-1602.
  27. Ouchi, N., Parker, J.L., Lugus, J.J., Walsh, K. 2011. Adipokines in inflammation and metabolic disease. Nature Reviews Immunology, 11(2): 85-97.
  28. Roussel, A.M., Hininger, I., Benaraba, R., Ziegenfuss, T.N., Anderson, R.A. 2009. Antioxidant effects of a cinnamon extract in people with impaired fasting glucose that are overweight or obese. Journal of the American College of Nutrition, 28(1):16-21.
  29. Sartorius, T., Peter, A., Schulz, N., Drescher, A., Bergheim, I., Machann, J. 2014. Cinnamon extract improves insulin sensitivity in the brain and lowers liver fat in mouse models of obesity. PloS One, 9(3): e92358.
  30. Shalaby, M.A., Saifan, H.A. 2014. some pharmacological effects of cinnamon and ginger herbs in obese diabetic rate. Journal of Intercultural Ethnopharmacology, 3(4): 144.
  31. Sindhu, S., Thomas, R., Shihab, P., Sriraman, D., Behbahani, K., Ahmad, R. 2015. Obesity is a positive modulator of IL-6R and IL-6 expression in the subcutaneous adipose tissue: significance for metabolic inflammation. PloS One, 10(7): e0133494.
  32. Tanaka, T., Narazaki, M., Kishimoto, T. 2014. IL-6 in inflammation, immunity, and disease. Cold Spring Harbor Perspective in Biology, 6(10): a016295.
  33. Teixeria, P., Going, S.B., Sardinha, L., Lohman, T.G., 2005. A review of psychosocial pre-treatment predictors of weight control. Obesity Reviews, 6(1): 43-65.
  34. Vieira, V.J., Valentine, R.J., Wilund, K.R., Antao, N., Baynard, T., Woods, J.A. 2009. Effects of exercise and low-fat diet on adipose tissue inflammation and metabolic complications in obese mice. American Journal of Physiology - Endocrinology and Metabolism, 296(5): 1164-1171.
  35. WHO. 2000. Obesity: preventing and managing the global epidemic, World Health Organization

فصلنامه زیست شناسی جانوری
دوره 13، شماره 2
زمستان 1399
صفحه 49-60

فایل ها

اشتراک گذاری

ارجاع به این مقاله

آمار