بررسی تغییرات شاخص های استرس اکسیداتیو و لیپیدی سرم به دنبال بیهوشی با پروپوفول در جوجه های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

چکیده

بروز استرس­اکسیداتیو از جمله عوارض جانبی بیهوشی می­باشد. هدف مطالعه فوق ارزیابی تغییرات شاخص­های استرس­اکسیداتیو به دنبال بیهوشی داخل­استخوانی با پروپوفول  (Propofol)در جوجه­های گوشتی بود. برای انجام مطالعه از 90 قطعه جوجه گوشتی نژاد راس استفاده شد. بیهوشی با تزریق 2 میلی­گرم پروپوفول به ازای هر کیلوگرم وزن پرنده، به‌صورت تزریق داخل­استخوانی جهت القای بیهوشی و 5/0 میلی­گرم پروپوفول به ازای هر کیلوگرم وزن پرنده، برای ادامه بیهوشی القا شد. جهت ارزیابی شاخص­های لیپیدی سرم و استرس­اکسیداتیو (فعالیت کاتالاز، غلظت مالون­دی­آلدئید و ظرفیت تام آنتی­اکسیدانی) خونگیری در مراحل قبل بیهوشی، حین بیهوشی و 24 ساعت بعد از بیهوشی انجام شد و سرم نمونه­های خون جدا شد. نتایج نشان داد میزان فعالیت آنزیم کاتالاز سرم 24 ساعت بعد از بیهوشی (69/6±5/129) به طور معنی­داری بیشتر از گروه کنترل و میزان آن در حین بیهوشی (04/5±9/112) و قبل از آن (21/4±9/120) بود. غلظت مالون­دی­آلدئید در حین بیهوشی افزایش نشان نداد (54/1±3/34) در حالی­که غلظت آن 24 ساعت بعد از بیهوشی افزایش معنی­داری داشت (28/4±8/42). ظرفیت آنتی­اکسیدانی تام 24 ساعت بعد از بیهوشی (67/1±2/19) در مقایسه با زمان قبل (27/1±9/25) و حین بیهوشی (37/1±4/27) به طور معنی­داری کاهش نشان داد. ظرفیت آنتی­اکسیدانی تام در قبل و حین بیهوشی نسبت به 24 ساعت پس از بیهوشی افزایش معنی­داری نشان داد. شاخص های لیپیدی سرم در طول آزمایش تغییراتی نشان نداد. به طور کلی نتایج این مطالعه نشان داد اگرچه بکارگیری پروپوفول در جوجه گوشتی در حین بیهوشی می تواند به طور موثری رادیکال­های آزاد را خنثی نماید و مانع از بروز اثرات جانبی استرس­اکسیداتیو گردد اما بعد از بیهوشی شاخص­های استرس­اکسیداتیو افزایش می یابد.

کلیدواژه‌ها


عنوان مقاله [English]

The Evaluation of Serum Lipids and Oxidative Stress Indices Following Propofol Administration in Broilers

نویسندگان [English]

  • Maryam Karimi Dehkordi
  • Majid Gholami-Ahangaran
  • Pardis Banimehdi
Department of Veterinary, ShareKord Branch, Islamic Azad University, ShareKord, Iran
چکیده [English]

The oxidative stress is one of the side effects of anesthesia. This study aimed to evaluate the changes in oxidative stress indices following propofol administration in broilers. For this purpose, 90 Ross broiler chicks were divided into two main and control groups. Anesthesia was induced by the injection of 2 mg propofol per kg of bird weight, intra-bone injection to induce anesthesia, and 0.5 mg propofol per kg bird weight to continuous anesthesia. To evaluate the serum lipids and oxidative stress indices (catalase activity, malondialdehyde concentration, and total antioxidant capacity), blood sampling was performed before anesthesia, during anesthesia, and 24 hours after the anesthesia. The results showed that the activity of serum catalase in 24 h after anesthesia (129.5 ± 6.69) was significantly higher than control chickens and its level during anesthesia (112.9 ±5.04), and before anesthesia (120.9 ± 4.21). The malondialdehyde concentration did not increase during anesthesia (34.3 ± 1.54), whereas it significantly increased 24 h after anesthesia (42.8 ± 4.28). Total antioxidant capacity significantly decreased 24 h after anesthesia (19.2 ± 1.67) compared to before (25.9 ± 1.27) and during anesthesia (27.4 ± 1.37). Total antioxidant capacity significantly increased before and during anesthesia compared to 24 h after anesthesia. Serum lipid indices did not change during the experiment. In general, this study's results showed that although using propofol in broiler chickens during anesthesia can effectively neutralize free radicals and prevent the side effects of oxidative stress, after anesthesia, oxidative stress indices increase.

کلیدواژه‌ها [English]

  • anesthesia
  • Oxidative stress
  • chickens
  • Propofol
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