مطالعه خصوصیات بیولوژیکی، ترکیبی و توپوگرافی سطحی داربست گرانوله هیدروکسی آپاتیت/ پوسته تخم شتر مرغ برای مهندسی بافت استخوان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد آیت ا... آملی، دانشگاه آزاد اسلامی، آمل، ایران

2 مرکز تحقیقات سلول بنیادی ، دانشگاه علوم پزشکی گلستان، گرگان، ایران

3 گروه تحقیقاتی بیومدیکال، انیستیتوی پژوهشی چیترا، دانشگاه پزشکی کرالاSCTIMST ، تیرو وانانتاپورام، هندوستان

چکیده

هدف از این مطالعه بررسی شکل هندسی و توانایی این داربست در ترکیب با سلول مزانشیمی و شبه فیبرو بلاستی و سنجش واکنش سمیت آن نسبت به سلول می­باشد. بدین لحاظ چند نسبت متفاوت از داربست هیدروکسی آپاتیت/ پوسته تخم شتر مرغ ایجاد گردید تا بتوان خصوصیات سطحی و پاسخگویی سلول را نسبت به انواع مختلف ترکیبی هیدروکسی آپاتیت/پوسته تخم شتر مرغ (OsE/HA) (نسبت­های 0-1، 1-2، 1-1، 2-، 1-0)  سنجید.  بیومتریال­های طبیعی منجمله پوسته تخم شترمرغ  که دارای مواد معدنی مثل کربنات کلسیم نقش عمده­ای در ساخت داربست مربوط به بافت­های سخت و تحقیقات امروزه را ایفا می­کنند. مشخصه یابی سطحی با استفاده از SEM و خصوصیات ترکیبی داربست بوسیله XRD  و FTIR مورد بررسی قرار گرفت و در نهایت برای کاربرد این ترکیب در بافت­های بیولوژیکی تست تماس مستقیم (سمیت) به­همراه سلول­های شبه فیبروبلاستی (L929) انجام گرفت. بعد از تست سمیت داربست آماده تست­های درون آزمایشگاهی (اینویترو) پذیرش سلول­های بنیادی مزانشیمی جدا شده از بافت چربی خرگوش سفید نیوزیلندی (RADMSCs) می­­گردد. نتایج این پژوهش نشان داد که این داربست دارای بهترین زیست­سازگاری و بیشترین لاکوناهای سطحی برای جذب سلول به خود در گروه هیدروکسی­آپاتیت/ پوسته تخم شتر مرغ با نسبت 2-1 می­باشد و این ترکیب بیومتریال طبیعی با مواد سنتتیک می­تواند در پیشبرد مهندسی  بافت استخوان کاربرد فراوان داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Study of Biological, compound and Surface Topography if Granule Scaffold Hydroxyapatite/Ostrich Eggshell for Bone Tissue Engineering

نویسندگان [English]

  • Farideh Alahgahi 1
  • Esmail Fattahi 1
  • Mohsen Saeedi 2
  • Mir Mahmoud Mortazavi Roudmiane 3
1 Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 Stem Cell Research Center, Golestan University of Medical sciences, Gorgan, Iran
3 Transmission Electron Microscope Lab, Biomedical Technology Wing, Sree Chitra Tirunal Institute
چکیده [English]

This study aimed to investigate the topography surface and ability of this scaffold in combination with the mesenchymal and fibroblast cells. In this regard, several different ratios of hydroxyapatite / ostrich eggshell scaffold were created to determine the surface properties and responsiveness to the cells with different types of hydroxyapatite/ostrich eggshell (OsE/HA) combinations (ratios 0-1, 1-2, 1-1, 2-1, 1-0). Natural biomaterials including ostrich eggshells which contain the minerals such as calcium carbonate, play a major role in the fabrication of hard tissue scaffolds. Surface characterization was investigated using SEM and recognizing the scaffold properties by XRD, FTIR. Finally, direct contact (toxicity) test with fibroblast-like cells (L929) was performed to study the cytotoxicity response. After the scaffold toxicity test, the material was ready for in vitro acceptance test, which mesenchymal stem cells isolated from the adipose tissue of New Zealand white rabbit (RADMSCs) which kept with this bioscaffold. This study's results showed that this scaffold has the best biocompatibility and the highest surface lacuna for cell adsorption and attachment with hydroxyapatite / ostrich eggshell. Bioscaffold OsE/HA with the ratio of 1-2 has the best cell attachment among other groups. Natural biomaterials with synthetic materials can promote the bone tissue engineering in many ways.

کلیدواژه‌ها [English]

  • Tissue Engineering
  • Hydroxyapatite scaffold
  • egg shell
  • ostrich
  • Fibroblast-like cells
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