تأثیر جایگزینی ویتامین E باتفاله انگور در جیره‌های حاوی چربی یا روغن بر عملکرد، فراسنجه‌های‌خونی، وضعیت آنتی‌اکسیدانی و ریخت شناسی روده باریک در جوجه های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ایران

2 گروه بیوشیمی، دانشگاه علوم پزشکی و خدمات درمانی استان لرستان، ایران

چکیده

این آزمایش به منظور بررسی تأثیر جایگزینی ویتامین E با تفاله انگور درجیره حاوی چربی یا روغن بر عملکرد، فراسنجه­های­خونی، وضعیت آنتی­اکسیدانی­ و ریخت شناسی روده کوچک جوجه­های گوشتی با استفاده از450 قطعه جوجه یک روزه سویه راس 308 (مخلوط دوجنس) در قالب طرح کاملا تصادفی با شش تیمار، پنج تکرار و15 قطعه جوجه درهرتکرار انجام شد. جیره های آزمایشی شامل: 1- شاهد روغن (جیره پایه به اضافه 5/3 درصد روغن سویا و ویتامینE، 2- شاهد پیه (جیره پایه به اضافه 5/3 درصد پیه و ویتامینE، 3- جیره حاوی5/3 درصد روغن و 9 درصد تفاله انگور، 4- جیره حاوی 5/3 درصد پیه و 9 درصد تفاله انگور ، 5- جیره حاوی 9 درصد تفاله انگور به اضافه 5/3 درصد روغن سویا و ویتامینE و 6- جیره حاوی 9 درصد تفاله انگور به اضافه 5/3 درصد پیه گاوی و ویتامینE بود. نتایج نشان داد که جایگزینی9 درصد تفاله انگور سفید بی دانه در جیره برعملکرد رشد،  مصرف خوراک،  ضریب تبدیل خوراک و شاخص تولید تاثیر ­معنی­­داری داشت و موجب کاهش معنی­دار وزن نسبی اجزای لاشه، کبد، قلب، سنگدان و چربی محوطه شکمی شد (05/0>p ).  مقدارگلوکز، کلسترول، اسیداوریک، تری­گلسیرید و لیپوپروتئین­با­چگالی بالا سرم خون تحت تاثیرجیره­های آزمایشی افزایش، در مقابل غلظت لیپوپروتئین­با­چگالی پایین کاهش یافت (05/0<P). افزودن تفاله انگور به جیره باعث افزایش فعالیت آنزیمهای سوپراکسیددیسموتاز، گلوتاتیون­پرا کیسداز، کاتالاز سرم خون شد. ولی غلظت مالون­دی­آلدئید را کاهش داد. علاوه بر­آن سبب تغییر خصوصیات مورفو­لوژی روده باریک شد. به طوری که ارتفاع پرزها، عمق کریپت، نسبت ارتفاع پرز به عمق کریپت و ضخامت دیواره روده کوچک کاهش یافت (05/0< p ). به طور کلی نتایج این مطالعه پیشنهاد می کند که گنجاندن 9 درصد تفاله انگور سفید در جیره جوجه­های گوشتی حاوی چربی اشباع و اشباع نشده سبب بهبود کیفیت لاشه و فراسنجه­های خونی می­شود، بدون این که تاثیرمنفی برعملکرد رشد جوجه­های گوشتی داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Replacement Vitamin E with Dietary Grape Pomace in Diets Containing Oil or Beef Tallow on Growth Performance, Blood Characteristics, Oxidative Status, and Gut Morphology of Broiler Chickens

نویسندگان [English]

  • Karim Ghorbani 1
  • Mohammad Reza Ghorbani 1
  • Ahmad Tatar 1
  • Hassan Ahmadvand 2
1 Department of Animal Sciences, Khuzestan University of Agricultural Sciences and Natural, Ahvaz, Iran
2 Department of Biochemistry, Lorestan University of Medical Sciences and Health Services, Khorramabad, Iran
چکیده [English]

To investigate the effects of replacement vitamin E with dietary grape pomace in the diets containing oil or beef tallow on growth performance, blood chara­cteristics, oxidative status, and gut morphology of broiler chickens, the present study was carried out. Four hundred and fifty-one-day-old broiler chickens were used in a completely randomized design with six treatments and five replicates. Experimental treatments were as follows; 1- control oil (CO, basal diet containing 3.5 percent soy oil) with 200 mg/kg vitamin E, 2-contraol tallow (CT, basal diet containing 3.5 percent tallow) with vitamin E, 3- CO with 9 percent grape pomace (GP), 4- CT with 9 % GP, 5- CO with 9 percent GP and vitamin E,6- CT with 9 % GP, and vitamin E. The results of this experiment showed that in the whole period, the birds fed diets containing soy oil, GP and vitamin E had the highest body weight gain and production index and the lowest feed conversion ratio compared to the others. The blood concentration of glucose, cholesterol, triglycerides, and high-density lipoproteins were reduced when GB was used in diets. Blood superoxide dismutase and glutathione peroxidase concentrations were increased, and malondialdehyde concentration was decreased when GP was used in broiler diets. In the duodenum part of the small intestine, villus height, crypt depth, villus height to crypt depth ratio, and muscularis thickness were increased when GP was used in broiler diets. To conclude, the result of this experiment suggests that although the GP has strong antioxidant properties, the simultaneous using vitamin E has a better effect on broiler growth performance.

کلیدواژه‌ها [English]

  • grape pomace
  • Blood biochemical parameters
  • broiler chicken
  • gut morphology
  • growth performance
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