تأثیر دوازده هفته تمرین هوازی بر آنزیم های آنتی اکسیدانی قلبی در موش های تغذیه شده با غذای کلسترول بالا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران

2 گروه پرستاری، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران

چکیده

امروزه چاقی به یک معضل جهانی تبدیل شده و از عوامل ایجاد کننده آن، کم تحرکی و رژیم غذایی سرشار از کلسترول است. این وضعیت باعث افزایش استرس اکسیداتیو و کاهش فعالیت آنزیم­های اکسیداتیو و در نتیجه عوارضی مانند بیماری­های قلبی عروقی می­شود. فعالیت ورزش هوازی و رعایت رژیم غذایی پرچرب به عنوان یک مداخله غیردارویی موثر بر افزایش ظرفیت آنتی اکسیدانی مورد استفاده قرار می­گیرد. هدف از مطالعه حاضر بررسی تأثیر 12 هفته تمرین هوازی بر آنزیم­های آنتی اکسیدانی قلبی در  موش­های تغذیه شده با غذای کلسترول بالا بود. 32 سر موش آزمایشگاهی نژاد ویستار مورد آزمایش قرار گرفتند. در نیمی از موش­ها با اضافه کردن یک درصد کلسترول به غذا طی 6 تا 8 هفته، هایپرکلسترولمی القا شد. موش­ها به 4 گروه هشت­تایی شامل تغذیه نرمال، گروه هایپرکلسترولمیک، تمرین هوازی- هایپرکلسترولمیک و تمرین هوازی- تغذیه نرمال تقسیم شدند. تمرین هوازی پنج روز در هفته و به مدت 12 هفته اجرا شد. 24 ساعت پس از آخرین جلسه تمرینی، موش­ها کشته و سلول­های قلبی خارج شدند. میزان فعالیت آنزیم­های سوپراکسید دیسموتاز، کاتالاز و گلوتاتیون پراکسیداز قلبی توسط کیت­های مخصوص ارزیابی شد. نتایج نشان داد که افزایش میزان کلسترول خون تاثیر آماری معنی­دار در کاهش آنزیم­ها در بافت قلب داشت. همچنین مشخص شد که تمرین هوازی باعث افزایش معنی­داری در میزان فعالیت آنزیم­های آنتی­اکسیدانی در بافت قلب می­شود. بیشترین میزان کاهش در سطح آنزیم­های آنتی­اکسیدانی مربوط به گروه هایپرکلسترولمی شده و ببیشترین میزان افزایش مربوط به گروه تمرینات هوازی – تغذیه نرمال بود. تغذیه سرشار از کلسترول ظرفیت آنتی اکسیدانی قلب را کاهش می­دهد. هرچند که تمرینات هوازی باعث افزایش فعالیت آنتی اکسیدانی قلب می­شود اما ظاهرا پرهیز از تغذیه سرشار از کلسترول می­تواند نقش موثرتری ایفا کند.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 12 Weeks of Aerobic Exercise on Cardiac Antioxidant Enzymes in the Hypercholesterolemic Male Wistar Rats

نویسندگان [English]

  • Soran Heidari 1
  • Kamal Azizbeigi 1
  • Kaveh Bahman pour 2
1 Department of Physical Education, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran
2 Department of Nursing, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran
چکیده [English]

Obesity has become a global problem today. Obesity can be caused by sedentary lifestyle and a high-cholesterol diet. This condition increases oxidative stress and decreases the activity of oxidative enzymes, resulting in many complications, including cardiovascular disorders. Aerobic exercise and avoidance of high-fat diets are used as a non-pharmacological intervention to increase antioxidant capacity; however, the exact mechanisms of this on the cardiovascular system have not yet been fully identified. The aim of this study was to evaluate the effect of 12 weeks of aerobic exercise on cardiac antioxidant enzymes in mice fed high cholesterol diet. Thirty-two laboratory Wistar rats (191.2 ± 19 g), age 6-8 weeks were randomly assigned to four groups after the hypercholesterolemia induction of half of the rats (adding 1% cholesterol to the food of the rats and sampling the tails of the rats). These four groups comprised of the aerobic exercise-normal nutrition group (n=8), hypercholesterolemic group (n=8), aerobic exercise- hypercholesterolemic group (n=8) and normal nutrition group (n=8). Each endurance exercise training session was held five days a week for 12 weeks. Twenty-four hours after the last training session, the rats were killed and the cardiomyocytes were removed. Moreover, the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) enzymes was evaluated by specific kits. The results showed that increasing blood cholesterol had a statistically significant effect on reducing SOD, CAT, and GPX in heart tissue (p=0.001). Furthermore, it was found that aerobic exercise significantly increases the activity of antioxidant enzymes in cardiomyocytes (p=0.001). The highest decrease in the level of antioxidant enzymes was related to the hypercholesterolemic group and the highest increase was related to the aerobic exercise-normal nutrition group. Cholesterol-rich diets reduce the activity of antioxidants in the heart. Although aerobic exercise increases the heart’s antioxidant activity, it seems that avoiding a high-cholesterol diet can play a more effective role.

کلیدواژه‌ها [English]

  • SOD
  • Cat
  • GPX
  • Aerobic exercise
  • High fat diet
  • Wistar Rat
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