اثر عصاره هیدروالکلی بوقناق (Eryngium campestre) بر تشنج ناشی از تزریق پنتیلن تترازول در موش‌های سوری نر بالغ

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه زیست شناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران

چکیده

صرع، شایع­ترین بیماری سیستم عصبی است. عوارض جانبی برخی داروها و مقاوم بودن نسبت به آنها، گیاهان را با هدف شناخت داروهایی با حداقل عوارض مورد توجه قرار داده است. این تحقیق به بررسی اثر عصاره هیدروالکلی بوقناق (Eryngium campestre) بر تشنج ناشی از پنتلین تترازول در موش سوری نر می­پردازد. در مطالعه تجربی حاضر 40 سر موش سوری نر نژاد ویستار با وزن 30-25 گرم به­طور تصادفی به 5 گروه 8 تایی شامل: سالین، 3 گروه صرعی درمان شده با دوزهای 50، 100 و 200 میلی­گرم بر کیلوگرم عصاره بوقناق و گروه کنترل مثبت (صرعی درمان شده با 1 میلی­گرم برکیلوگرم دیازپام) تقسیم شدند. جهت ایجاد مدل صرع، 85 میلی­گرم بر کیلوگرم پنتیلن تترازول (PTZ) تزریق گردید. تجویز عصاره و یا دارو30 دقیقه قبل از تجویز پنتیلن تترازول صورت گرفت. میزان تأثیر دوزهای مختلف عصاره و دارو بر شدت تشنج با اندازه­گیری مدت زمان بروز حالت­های مختلف رفتاری ناشی از تشنج در حیوان ارزیابی گردید. همه تجویزها به­صورت درون صفاقی انجام گرفت. گروه­های درمان شده با دوزهای مختلف عصاره بوقناق و همچنین گروه درمان شده با دیازپام، افزایش معنی­دار را در زمان تأخیر در شروع تشنج، مدت زمان تونیک، مدت زمان کلونیک، مدت زمان تونیک-کلونیک، طول زمان تشنج و میزان زنده ماندن در مقایسه با گروه سالین نشان می­دهند. نتایج حاصل از این تحقیق نشان داد عصاره بوقناق سبب افزایش تأخیر در شروع تشنج و کاهش مرگ­ومیر ناشی از تشنج در موش‌های سوری می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Eryngium Campestre Hydroalcoholic Extract on Seizure Due to by Pentylene Tetrazole Injection in Adult Male Rats

نویسندگان [English]

  • Sara Zandi
  • Saeid Valipour Chahardahcharic
  • Maryam Rafieirad
Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
چکیده [English]

Epilepsy is the most common disorders of the nervous system. The side effects of some drugs and their resistance to them have been considered by plants for identifying drugs with minimal side effects. This study investigated the effect of hydroalcoholic extract of E. campestre on pentylenetetrazol-induced seizures in male mice. In the present experimental study, 40 male Wistar rats weighing 25-30 g were randomly divided into 5 groups (n = 8): saline, three epileptic groups treated with doses of 50, 100, and 200 mg/kg of E. campestre extract and positive control group treated with 1 mg/kg diazepam. In order to create a model of epilepsy, Pentylenetetrazol (PTZ) was injected with dose of 85 mg/kg. The effect of different doses of extract and drug on the severity of seizures was evaluated by measuring the duration of different seizure behavioral states in the animal. All prescriptions were given intraperitoneally (ip). The groups treated with different doses of E. campestre extract as well as the diazepam treated group showed a significant increase in delayed onset of seizure, tonic duration, clonic duration, and tonic-clonic duration, and length Seizure time and survival rate were significantly higher in the saline group. The results of this study showed that E. campestre extract increase delayed onset of seizure and reduced mortality due to seizure in mice.

کلیدواژه‌ها [English]

  • Eryngium Campestre
  • Mice
  • Pentylene Tetrazole
  • Seizure
  1. Akinmoladun A.C., Ibukun E., Afor E., Obuotor E., Farombi E. 2007. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Scientific Research and Essay, 2(5): 163-166.
  2. Alimohammadi B., Azhdari-Zarmehr H., Sofiabadi M. 2014. Anticonvulsant effect of hydroalcoholic extract of scrophularia striata boiss. On pentylenetetrazol-induced seizure in mice. Journal ofKerman Universityof Medical Sciences, 21(3): 207-218. [In persian]
  3. Arzi A., Kesmati M., Alikhani M. 2004. Preventive effect of hydroalcoholic extract of Matricaria Chamomilla on Nicotine induced convulsions in mice. Journal of Babol Universityof Medical Sciences, 6(2): 12-17.
  4. Azhdari-Zarmehri H., Naderi F., Erami E., Mohammad-Zadeh M. 2013. Effects of Salvia Sahendica hydroalcoholic extract on PTZinduced seizure in male mice. Koomesh, 14(4): 497-504.
  5. Becker A., Grecksch G., Brosz M. 1995. Antiepileptic drugs—their effects on kindled seizures and kindling-induced learning impairments. Pharmacology Biochemistry and Behavior, 52(3): 453-459.
  6. Bum E.N., Taiwe G., Moto F., Ngoupaye G., Vougat R., Sakoue V., et al. 2011. In Clinical and genetic aspects of epilepsy: Published byIntechOpen. Chapter 8: 175-192.
  7. Cárdenas-Rodríguez N., Coballase-Urrutia E., Rivera-Espinosa L., Romero-Toledo A., Sampieri III A., Ortega-Cuellar D., et al. 2013. Modulation of antioxidant enzymatic activities by certain antiepileptic drugs (valproic acid, oxcarbazepine, and topiramate): evidence in humans and experimental models. OxidativeMedicineand Cellular Longevity, 2598493: 1-8.
  8. Choudhary N., Bijjem K.R.V., Kalia A.N. 2011. Antiepileptic potential of flavonoids fraction from the leaves of Anisomeles malabarica. Journal of Ethnopharmacology, 135(2): 238-242.
  9. Costa Marques T.H., Santos De Melo C.H., Fonseca De Carvalho R.B., Costa L.M., De Souza A.A., David J.M., et al. 2013. Phytochemical profile and qualification of biological activity of an isolated fraction of Bellis perennis. Biological Research, 46(3): 231-238.
  10. Dawilai S., Muangnoi C., Praengamthanachoti P., Tuntipopipat S. 2013. Anti-inflammatory activity of bioaccessible fraction from Eryngium foetidum leaves. BioMed research international, 2013(6): 958567.
  11. Elisabetsky E., Brum L.S., Souza D. 1999. Anticonvulsant properties of linalool in glutamate-related seizure models. Phytomedicine, 6(2): 107-113.
  12. Erdelmeier C., Sticher O. 1985. Coumarin Derivatives from Eryngium campestre1. Planta Medica, 51(05): 407-409.
  13. Esmaeili M.A., Sonboli A., Noushabadi M.A. 2010. Antioxidant and protective properties of six Tanacetum species against hydrogen peroxide-induced oxidative stress in K562 cell line: A comparative study. Food Chemistry, 121(1): 148-155.
  14. Esmaeili S., Taene A., Hemmati M., Malekaneh M. 2017. Effect of aqueous extract of eryngium campestre on the prevention of pathologic alterations caused by calcium oxalate crystals induced by ethylene glycol in the cortex and medulla of rats kidneys. Journal of Birjand University of Medical Sciences, 24 (Supplementary): 84-92.
  15. Güneş M.G., İşgör B.S., İşgör Y.G., Moghaddam N.S., Geven F., Yildirim Ö. 2014. The effects of Eryngium campestre extracts on glutathione-s-transferase, glutathione peroxidase and catalase enzyme activities.Turkish Journal of Pharmaceutical Sciences, 11(3): 339-346.
  16. Hohmann J., Pall Z.; Günther G., Mathe I. 1997. Flavonolacyl glycosides of the aerial parts of Eryngium campestre. Planta medica, 63(01): 96-96.
  17. Huang R.Q., Bell-Horner C.L., Dibas M.I., Covey D.F., Drewe J.A., Dillon G.H. 2001. Pentylenetetrazole-induced inhibition of recombinant γ-aminobutyric acid type A (GABAA) receptors: mechanism and site of action. Journal of Pharmacology and Experimental Therapeutics, 298(3): 986-995.
  18. Kartal M., Mitaine-Offer A.C., Paululat T., Abu-Asaker M., Wagner H., Mirjolet J.F., et al. 2006. Triterpene saponins from Eryngium campestre. Journal of natural products, 69(7): 1105-1108.
  19. Kartnig T., Wolf J. 1993. Flavonoide aus den oberirdischen Teilen von Eryngium campestre. Planta Medica, 59(03): 285-285.
  20. Katyal J., Kumar H., Gupta Y.K. 2015. Anticonvulsant activity of the cyclooxygenase-2 (COX-2) inhibitor etoricoxib in pentylenetetrazole-kindled rats is associated with memory impairment. Epilepsy and Behavior, 44: 98-103.
  21. Miraj, S., Alesaeidi S. 2016. A systematic review study of therapeutic effects of Matricaria recuitta chamomile (chamomile). Electronic physician, 8(9): 3024-3031.
  22. Mosefi M., Mehrabani M., Zolhasab H. 2004. Antimicrobial effects of Iranian salvia and Azerbaijani salvia extracts on six strains of gram-positive and gram-negative bacteria. Journal ofKerman Universityof Medical Sciences, 11(2): 109-18. [In Persian]
  23. Nassiri-Asl M., Shariati-Rad S., Zamansoltani F. 2008. Anticonvulsive effects of intracerebroventricular administration of rutin in rats. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 32(4): 989-993.
  24. Okoye T., Akah P., Omeke C. 2010. Evaluation of the anticonvulsant and muscle relaxant effects of the methanol root bark extracts of Annona senegalensis. Asian Pacific Journal of Tropical Medicine, 3(1): 25-28.
  25. Quintans Júnior L.J., Almeida J.R., Lima J.T., Nunes X.P., Siqueira J.S., Oliveira L.E.G.d. et al. 2008. Plants with anticonvulsant properties: a review. Revista Brasileira de Farmacognosia, 18: 798-819.
  26. Ramalingam R., Nath A. R., Madhavi B.B., Nagulu M., Balasubramaniam A. 2013. Free radical scavenging and antiepileptic activity of Leucas lanata. Journal of Pharmacy Research, 6(3): 368-372.
  27. Reid C.A., Jackson G.D., Berkovic S.F., Petrou S. 2010. New therapeutic opportunities in epilepsy: a genetic perspective. Pharmacology and therapeutics, 128(2): 274-280.
  28. Rojas A., Jiang J., Ganesh T., Yang M.S., Lelutiu N., Gueorguieva P., Dingledine R. 2014. Cyclooxygenase‐2 in epilepsy. Epilepsia, 55(1): 17-25.
  29. Salmani M., Mirnajafizadeh J., Fathollahi Y. 2007. Offsetting of aberrations associated with seizure proneness in rat hippocampus area CA1 by theta pulse stimulation–induced activity pattern. Neuroscience, 149(3): 518-552.
  30. Sander J.W. 2003. The epidemiology of epilepsy revisited. Current Opinion in Neurology, 16(2): 165-170.
  31. Shahbazi Y., Shavisi N., Karami N., Kakaei S. 2015. Chemical composition and in vitro antibacterial activity of Ferulago angulata (Schlecht.) Boiss essential oil. Pharmaceutical Sciences, 21(1): 6.-11
  32. Stafstrom C.E., Carmant L. 2015. Seizures and epilepsy: an overview for neuroscientists. Cold Spring Harbor perspectives in medicine, 5(6): a022426.
  33. Thurman D.J., Beghi E., Begley C.E., Berg A.T., Buchhalter J.R., Ding D., et al. 2011. Standards for epidemiologic studies and surveillance of epilepsy. Epilepsia, 52:(suppl.7): 2-26.