بررسی تغییرات بیانmiR-1193 در نمونه های بافت بیماران مبتلا به گلیوبلاستوما مولتی فرم

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

چکیده

گلیوبلاستوما مولتی فرم یکی از بدخیم­ترین سرطان­ها می­باشد که با توجه به دشوار بودن تشخیص زود متاسفانه اکثر بیماران طی یک سال بعد از تشخیص می­میرند.RNA های غیرکدکننده نوعی از RNAها هستند که هیچ پروتئینی را رمزگذاری نمی­کنند و به عنوان تنظیم­کنندهای پس از رونویسی در بیان ژن عمل کرده و می­توانند نقش مهمی را تشخیص سرطان داشته باشند. هدف از این مطالعه بررسی تغییرات بیان miR-1193  و ارتباط آن با گرید، سن و جنسیت می­باشد. در این مطالعه تغییرات بیان miR-1193  را در 50 نمونه بافت مبتلا به گلیوبلاستوما مولتی فرم در مراحل مختلف بیماری و 50 نمونه نرمال با تکنیکReal-Time PCR  که برای آنالیز میزان بیان ژن­های مورد مطالعه می­باشد و از GraphPad Prism 8 برای آنالیز آماری استفاده شد همچنین با اسفاده از منحنیRoc  ارزش بیومارکی زیستی آن مورد ارزیابی قرار گرفت. نتایج حاصل از این مطالعه نشان داد سطح بیانmiR-1193  افزایش بیان را  در نمونه­های بیماران مبتلا به گلیوبلاستوما مولتی فرم نسبت به نمونه های سالم نشان داد. همچنین بیان ژن mir11933-5p از لحاظ آماری با Grade و سن و جنسیت بیمار ارتباط معنی­دار نداشت. با آنالیز منحنی و تعیین مقدار cut-off  برای این بیومارکر، مشخص گردید که میزان off-cut  برابر با 141/5 برای  miR-1193بود که بیماران را با اختصاصیت 1 و حساسیت 78/0 می­تواند تشخیص دهد. اما با توجه به میزان حساسیت به نسبت پایین آن   باید بررسی بیشتری صورت بگیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Changes in the Expression of miR-1193 in Tissue Samples of Patients with Glioblastoma Multiform

نویسندگان [English]

  • Ali Hoseinyazdi
  • Elham Moslemi
  • Vajiheh Zarrinpour
  • Mohammad Mahdi Forghanifard
Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
چکیده [English]

Glioblastoma is one of the most malignant cancers worldwide, which due to the difficulty of early diagnosis, most affected people die within a year after diagnosis. Non-coding RNAs are a group of RNAs acting as post-transcriptional regulators and can play an essential role in cancer diagnosis. This study was aimed at investigating the expression level of miR-1193 in glioblastoma multiform and normal tissue samples and their correlation with the grade, age, and sex parameters. In this study, the expression level of miR-1193 was quantified in 50 glioblastoma multiform tissues (at different stages of disease), and 50 normal ones using the Real-Time PCR technique. GraphPad Prism 8 software was used for statistical analysis. The results of this study revealed down-regulation of the miR-1193 glioblastoma multiform samples compared to normal ones. The expression of miR-1193 was not significantly correlated with grade, age, and sex parameters. Finally, increased expression of miR-1193 could play an essential role in glioblastoma multiform. Moreover, according to Roc curve analysis and determining the cut-off value for this biomarker, it was found that the off-cut value was equal to 5.141 for miR-1193, which can detect patients with specificity of 1 and sensitivity of 0.78. However, due to its relatively low sensitivity, more research should be conducted.

کلیدواژه‌ها [English]

  • Glioblastoma Multiform
  • miR-1193
  • expression
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