اثر تزریق درون تخم مرغی نانوذرات نقره بر پاسخ سیستم ایمنی جوجه های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گلستان، گرگان، ایران

2 گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران، ساری، ایران

3 دامپزشک، مدیر نمایندگی سازمان مدیریت صنعتی گلستان، گلستان، گرگان، ایران

چکیده

در این بررسی اثر تزریق درون تخم­مرغی نانوذرات نقره بر پاسخ سیستم ایمنی جوجه­های گوشتی مورد مطالعه قرار گرفت.  در این راستا 560 عدد تخم­مرغ در چهار گروه با چهار تکرار به صورت تصادفی تقسیم شدند. گروه­های آزمایشی شامل:1) کنترل مثبت (تزریق 1 میلی­لیتر سرم فیزیولوژی)، 2) کنترل منفی (بدون تزریق سرم فیزیولوژی)، 3) تزریق20 میلی‌گرم نانوذرات نقره و 4) تزریق40 میلی­گرم نانو ذرات نقره به ازای هر تخم­مرغ بود. تزریق نانوذرات نقره و گروه کنترل در روز 7 دوره جوجه­کشی انجام گرفت. در انتهای دوره جوجه­کشی، جوجه­ها جهت بررسی درصد جوجه­درآوری شمارش شده و پس از وزن­کشی جهت بررسی پارامترهای سیستم ایمنی کشتار شدند. درصد جوجه­درآوری در گروه­های آزمایشی تفاوت معنی­داری را داشت (05/0 > p). به طوری که گروه کنترل منفی بیشترین درصد جوجه­درآوری را نشان داد. نانوذرات نقره موجب بهبود رشد جوجه­ها در طی دوران جوجه­کشی شد (05/0 > p). به طوری که گروه دریافت کننده 20 میلی­گرم نانوذرات نقره بیشترین وزن بدن را نشان داد. نانوذرات نقره وزن نسبی کبد و طحال را به طور معنی­داری افزایش داد (05/0 > p). غلظت ایمونوگلوبین G ((IgG، M (IgM)، تعداد کل گلبول­های سفید و نسبت هتروفیل به لنفوسیت تفاوت معنی­داری را نشان نداند (05/0 < p). بیان ژن فاکتور نکروز تومور کبدی آلفا (TNF-α)، اینترلوکین6 (6IL-)، فاکتور رشد بتا در حال تغییر (TGF-β) و فاکتور رشد شبه انسولینی1 (IGF-1) در گروه­های آزمایشی تفاوت معنی­داری را نشان دادند (05/0 >p ). به طوری که گروه دریافت کننده 20 میلی­گرم نانوذرات نقره بالاترین سطح بیان ژن را نشان داد. به طور کلی نتایج حاصل از این مطالعه نشان داد تزریق درون تخم­مرغی نانوذرات نقره سبب تقویت سیستم ایمنی جوجه­های گوشتی می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Ovo Injection of Silver Nanoparticles on Immune System Response in Broiler Chickens

نویسندگان [English]

  • Samira Arabameri 1
  • Firooz Samadi 1
  • Behrooz Dastar 1
  • Zarbakht Ansari 2
  • Reza Mobaseri 3
1 Department of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Gorgan, Iran
2 Department of Animal Science, Sari University of Agricultural Sciences and Natural Resources, Mazandaran, Sari, Iran
3 Vet, Manager of Golestan Branch of IMI, Golestan, Gorgan, Iran
چکیده [English]

In this study, the effect of ovo injection of silver nanoparticles on the immune system response of broilers was studied. In this regard, 560 eggs were randomly divided into four groups with four replications. Experimental groups included: 1) positive control (injection of 1 mL of physiological serum on the seventh day of incubation), 2) negative control (no injection of physiological serum), 3) injection of 20 mg silver nanoparticles, and 4) injection of 40 mg silver nanoparticles per it was an egg. The injection of silver nanoparticles and the control group was performed on day 7 of the incubation period. At the end of the incubation period, the chicks were counted to check the percentage of hatchability and after weighting, they were slaughtered to check the parameters of the immune system. There was a significant difference in hatching percentage in experimental groups (p <0.05) so that the negative control group showed the highest percentage of hatching chickens. Silver nanoparticles improved the growth of chickens during the incubation period (p <0.05) so that the group receiving 20 mg of silver nanoparticles showed the highest body weight. Silver nanoparticles significantly increased the relative weight of liver and spleen (p <0.05). Immunoglobulin G (IgG, M (IgM) concentration, total white blood cell count, and heterophile to lymphocyte ratio did not show significant differences (p >0.05). Hepatic tumor necrosis factor-alpha (TNF-α) gene expression Interleukin (6) (IL-, beta-modifying growth factor (TGF-β)) and insulin-like growth factor-1 (IGF-1) showed significant differences in the experimental groups (p <0.05). The group receiving 20 mg of silver nanoparticles showed the highest level of gene expression. In general, the results of this study showed that ovo injection of silver nanoparticles strengthens the immune system of broilers.

کلیدواژه‌ها [English]

  • Gene expression
  • immune response
  • Ovo Injection
  • chicken
  • silver nanoparticles
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