بررسی اثر هارمالین بر سطح سرمی آنزیم های شاخص کبدی در مدل کبد چرب غیرالکلی موش های نر نژاد NMRI

نوع مقاله : مقاله پژوهشی

نویسندگان

ﮔﺮوه زیست شناسی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران

چکیده

یکی از شایع­ترین اختلالات کبدی در حال حاضر، کبد چرب می باشد که امروزه به‌دلیل سبک نادرست زندگی، مبتلایان به آن در جهان رو به افزایش است. هدف از پژوهش حاضر، بررسی تاثیر ماده موثره گیاه اسپند یعنی هارمالین، بر سطوح سرمی آنزیم­های شاخص کبدی در موش‌های مبتلا به کبد چرب غیرالکلی می­باشد. در این تحقیق از 40 سر موش نر بالغ نژاد NMRI که به 5 گروه 8تایی شامل گروه های کنترل، HFD،  شم (HFD دریافت کننده آب مقطر که حلال هارمالین می­باشد)، دریافت کننده هارمالین 50 و 100 میلی­گرم بر کیلوگرم تقسیم شدند، استفاده شد. هارمالین به صورت داخل صفاقی و به مدت 4 هفته تزریق شد. برای ارزیابی عملکرد کبد، سطوح سرمی آلانین آمینوترانسفراز (ALT)، آسپارتات آمینوترانسفراز (AST)، آلکالین فسفاتاز (ALP) و پروفایل لیپیدی (کلسترول، LDL و HDL) اندازه گیری شدند. میزان فعالیت آنزیم های آنتی  اکسیدانی مانند کاتالاز، گلوتاتیون پراکسیداز و سوپراکسیددیسموتاز (SOD) در بافت کبد نیز بررسی شدند. بررسی­های هیستولوژیکی با استفاده از رنگ آمیزی هماتوکسیلین-ائوزین در بافت کبد انجام شد. سطوح سرمی ALT، AST، ALP، کلسترول و LDL به طور معنی­داری در گروه­های تیمارشده با هارمالین کاهش و سطوح آنزیم­های آنتی اکسیدان کاتالاز، گلوتاتیون پراکسیداز و SOD بطور معنی­داری در گروه­های تیمار شده افزایش یافت. میزان HDL بطور معنی­داری در گروه­های تیمار شده با هارمالین افزایش معنی­دار یافت. نتایج به دست آمده، اشاره به خواص آنتی اکسیدانی هارمالین دارد که از مهمترین آلکالوئیدهای گیاه اسپند می باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Effect of Harmaline on the Serum Level of Liver Index Enzymes in a Model of Non-alcoholic Fatty Liver of Male NMRI Mice

نویسندگان [English]

  • Tannaz Kowsari Moghadam
  • Hanieh Jafary
  • Parichehreh Yaghmaei
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Right now, one of the most common liver disorders is fatty liver, on the rise in the world today due to the poor lifestyle of those suffering from it. The aim of this study was to investigate the effect of the active ingredient of Peganum harmala, harmaline, on serum levels of liver index enzymes in mice with non-alcoholic fatty liver. In this study, 40 adult male NMRI mice were divided into five groups of 8, including control, HFD, sham (HFD received distilled water, a harmaline solvent.), harmaline receiving 50 mg/kg and 100 mg/kg. Harmaline was injected intraperitoneally for four weeks. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lipid profile (cholesterol, LDL, and HDL) were measured to assess liver function. The activity of antioxidant enzymes such as catalase, glutathione peroxidase, and superoxide dismutase (SOD) in liver tissue were evaluated. Histological studies were performed using Hematoxylin-Eosin staining in liver tissue. Serum levels of ALT, AST, ALP, cholesterol, LDL were significantly decreased in the groups treated with harmaline and the levels of antioxidant enzymes catalase, glutathione peroxidase, and SOD were significantly increased in the treated groups. HDL levels increased significantly in harmaline-treated groups. The results indicated the antioxidant properties of harmaline, one of the most important alkaloids of Peganum harmala.
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کلیدواژه‌ها [English]

  • Harmaline
  • Antioxidant
  • Nonalcoholic Fatty Liver
  • Liver Enzyme
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