اثرات ژرانیول و ورزش بر حافظه و یادگیری در موش های نر صحرایی آلزایمری شده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه بیوشیمی، مرکز تحقیقات بیوشیمی و بیوفیزیک، دانشگاه تهران، تهران، ایران

3 مرکز تحقیقات بیوسنسور، پژوهشکده علوم سلولی و مولکولی غدد و متابولیسم، دانشگاه علوم پزشکی تهران، تهران، ایران

4 مرکز تحقیقات غدد و متابولیسم، پژوهشکده علوم بالینی غدد و متابولیسم، دانشگاه علوم پزشکی تهران، تهران، ایران

چکیده

بیماری آلزایمر از مهمترین بیماری­های تحلیل برنده عصبی است که به دنبال تولید رادیکال­های آزاد و فاکتور‌های التهابی بروز می­کند. ژرانیول که یک مونوترپن طبیعی است دارای اثرات آنتی­اکسیدانی و ضدالتهابی می­باشد. هدف از این مطالعه، بررسی اثرات ژرانیول و ورزش بر حافظه و یادگیری، میزان فعالیت SOD و سطح MDA، میزان نوروژنز و نیز تعداد پلاک­های آمیلوئیدی در موش­های آلزایمری شده نژاد ویستار می­باشد. برای ایجاد مدل آلزایمری، از تزریق دو طرفه  Aβ 1-42درون هیپوکامپ رت­های نر استفاده شد. گروه­بندی شامل: کنترل منفی، کنترل مثبت، ورزش، آلزایمری، آلزایمری + ورزش، آلزایمری + ژرانیول، آلزایمری + ژرانیول + ورزش می­باشد. نتایج نشان داد که میزان فعالیت SOD و سطح سرمی MDA در گروه­های ورزش، درمانی و دریافت کننده ژرانیول توام با ورزش در مقایسه با سایر گروه­ها به ترتیب افزایش و کاهش معناداری پیدا کردند (001/0>P). نتایج تست رفتاری شاتل باکس نیز نشان داد در مدت زمان ورود به محفظه تاریک، بین گروه­های درمانی و دریافت کننده ژرانیول توام با ورزش در مقایسه با گروه­های آلزایمری و آلزایمری توام با ورزش در سطح (001/0>P) افزایش معناداری وجود داشت. همچنین میزان نوروژنز درگروه­های درمانی و ژرانیول توام با ورزش در مقایسه با گروه­های آلزایمری و آلزایمری توام با ورزش در سطح (001/0>P) افزایش معناداری یافته بود. در تعداد پلاک­های آمیلوئیدی درگروه­های درمانی و ژرانیول توام با ورزش در مقایسه با سایر گروه­ها نیز در سطح (001/0>P) کاهش معناداری مشاهده گردید. با توجه به نتایج به دست آمده در این تحقیق به نظر می­رسد استفاده توام ژرانیول و ورزش می­توانند اختلالات ایجاد شده در حافظه،  میزان فعالیت SOD، سطح MDA، میزان نوروژنز و نیز پلاک­های آمیلوئیدی ایجاد شده در بیماری آلزایمر را به طور چشمگیری بهبود بخشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Geraniol and Exercise on Memory and Learning in Male NMRI Alzheimer’s Rats

نویسندگان [English]

  • Bahareh Seifi Nahavandi 1
  • Parichehr Yaghmaee 1
  • Shahin Ahmadian 2
  • Maryam Ghobeh 1
  • Azadeh Ebrahim-Habibi 3 4
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
3 Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
4 Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
چکیده [English]

Alzheimer’s disease is one of the most important neurodegenerative diseases occurring after the production of free radicals and inflammatory factors. Geraniol, which is a natural monoterpene, contains antioxidant and anti-inflammatory effects. The aim of this study was to investigate the effects of Geraniol and exercise on memory and learning, SOD activity and MDA serum level, neurogenesis and amyloid plaque count in Alzheimer’s Wistar rats. To create the Alzheimer's disease model, Aβ 1-42 was injected bilaterally into the hippocampus of male rats. Grouping included control (-), control (+), exercise, Alzheimer, Alzheimer + exercise, Alzheimer + Geraniol, and Alzheimer + Geraniol + exercise. The results showed that SOD activity and MDA serum level were significantly increased in the Alzheimer + Geraniol, Alzheimer + exercise, and Alzheimer + Geraniol + exercise groups compared to other groups (P<0.001). In addition, shuttle box behavioral test demonstrated that the entry time to the dark box was notably increased in Alzheimer + Geraniol and Alzheimer + Geraniol + exercise groups compared to Alzheimer and Alzheimer + exercise groups (P<0.001). Moreover, neurogenesis was remarkably increased in Alzheimer + Geraniol and Alzheimer + Geraniol + exercise groups compared to Alzheimer and Alzheimer + exercise groups (P<0.001). Besides, the number of amyloid plaques was decreased Alzheimer + Geraniol and Alzheimer + Geraniol + exercise groups compared to other groups (P<0.001). Based on the results of this study, it seems that this compound along with exercise is notably capable of improving complications in memory, SOD activity, MDA serum level, and neurogenesis as well as amyloid plaques in Alzheimer’s disease.

کلیدواژه‌ها [English]

  • Alzheimer’s
  • Geraniol
  • Exercise
  • SOD
  • MDA
  • Neurogenesis
  • Amyloid Plaques
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