اثر هشت هفته تمرینات استقامتی در شیب مثبت و منفی همراه با مصرف ژل رویال بر غلظت هیپوکامپی پروتئین کربونیل و آستانه تحمل درد موش‌های صحرایی آلزایمری شده با تری متیل تین

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

چکیده

افزایش استرس اکسیداتیو موجب آسیب نورونی و بروز دردهای مزمن در بیماران مبتلا به آلزایمر می­شوند. مطالعه حاضر با هدف بررسی اثر هشت هفته تمرینات استقامتی در شیب مثبت (PST) و منفی (NST) همراه با ژل رویال (RJ) بر غلظت هیپوکامپی پروتئین­کربونیل (PC) و آستانه تحمل درد (PT) موش­های صحرایی آلزایمری شده با تری­متیل­تین (TMT) انجام شد. در این مطالعه تجربی 49 سر موش­ صحرایی آلزایمری شده با تزریق درون صفاقی 8 میلی­گرم بر کیلوگرم نوروتوکسین TMT به طور تصادفی به گروه­های هفت­تایی شامل: 1- AD، 2- شم (نرمال­سالین) (Sh)، 3- PST، 4- NST، 5- PST+RJ، 6- NST+RJ و 7- RJ تقسیم  شدند. جهت بررسی اثر بیماری آلزایمر بر متغیرهای تحقیق 7 سر موش صحرایی در گروه کنترل سالم (C) قرار گرفتند. PST (با سرعت 16 متر بر دقیقه در شیب مثبت، (15%+) و NST (با سرعت 16 متر بر دقیقه در شیب منفی (15% -) به مدت هشت هفته، پنج جلسه در هفته، هر جلسه 60 دقیقه انجام شد. مصرف RJ (حل شده در نرمال­سالین) به مدت هشت هفته  mg/kg/day100 بصورت صفاقی انجام شد. برای بررسی تفاوت بین گروهی از آزمون آنالیز واریانس یک راهه همراه با آزمون تعقیبی توکی در نرم افزار SPSS 22 انجام شد (05/0≥p ). NST موجب کاهش سطوح هیپوکامپی PC (001/0=p ) و PT گردید، همچنین PST موجب کاهش PT (001/0= p) گردید. NST+RJ موجب کاهش سطوح هیپوکامپی PC (001/0=p ) و PT (001/0=p ) در موش­های صحرایی مبتلا به بیماری آلزایمر گردید. همچنین PT در گروه PST+RJ به طور معنی­داری کاهش یافت(001/0= p). سطوح هیپوکامپی PC در گروه NST+RJ پایین­تر از گروه PST+RJ بود (001/0=p ). به نظر می­رسد NST به تنهایی و همراه با RJ دارای اثرات کاهنده استرس اکسیداتیو و ضد دردی می­باشند، هر چند تعامل PST و RJ اثرات ضد دردی داشت، ولی مطالعات بیشتری در زمینه اثرات کاهنده­ی استرس اکسیداتیو متعاقب PST و مصرف RJ احساس می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Eight Weeks of Endurance Training on Positive and Negative Gradients with Royal Jelly on Hippocampal Concentration of Carbonyl Protein and Pain Tolerance Threshold of Trimethylettin-treated Alzheimer’s Rats

نویسندگان [English]

  • Hamid Mousazadeh
  • Nematollah Nemati
  • Tahereh Bagherpoor
Department of Physical Education and Sport Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
چکیده [English]

Increased oxidative stress causes neuronal damage and incidence of chronic pains in patients with Alzheimer’s disease (AD). The aim of this study was to investigate the effect of eight weeks of endurance exercises in positive (PST) and negative (NST) slope with royal jelly (RJ) on the hippocampal concentration of protein-carbonyl (PC) and pain tolerance threshold (PT) of Alzheimer’s rats with trimethyltin (TMT). In this experimental study, 49 Alzheimer’s rats with intraperitoneal injection of 8 mg/kg neurotoxin TMT were randomly divided into seven groups :(1) AD, (2) sham (normal saline) (Sh), (3) PST, (4) NST, (5) PST + RJ, (6) NST + RJ, and (7) RJ. To investigate the effect of Alzheimer’s disease on research variables, seven rats were included in the healthy control group (C). PST (with the speed of 16 m/min at positive slope (+15%)) and NST (with the speed of 16 m/min at negative slope (-15%)) interventions were carried out for eight weeks, five sessions per week and 60 minutes per session. RJ (dissolved in normal saline) was intraperitoneally consumed for eight weeks at 100 mg/kg /day. One-way ANOVA test along with Tukey post-hoc test were used in SPSS 22 software to investigate the intergroup difference (P ≤0.05). NST caused to decrease hippocampal level of PC (P = 0.001) and PT, as well as PST caused to decrease PT (P = 0.001). NST + RJ caused to decrease the hippocampal levels of PC (P = 0.001) and PT (P = 0.001) in rats with Alzheimer’s disease. Moreover, PT significantly reduced in the PST + RJ group (P = 0.001). PC hippocampal levels in the NST + RJ group were lower than in the PST + RJ group (P = 0.001). It seems that NST alone and with RJ have analgesic (anti-pain) and oxidative stress reduce effects. Although the interaction of PST and RJ had analgesic effects, the need of more studies on oxidative stress reducing effects is felt following PST and RJ consumption.

کلیدواژه‌ها [English]

  • Exercise
  • royal jelly
  • Carbonyl Protein
  • Pain Tolerance Threshold
  • Alzheimer
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