پاسخ مایوکاین‌های MyoD و MRF4 به فعالیت بدنی اکسنتریک و کانسنتریک در مردان جوان سالم

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

هدف از پژوهش حاضر بررسی پاسخ مایوکاین­های MyoD و MRF4 به فعالیت بدنی اکسنتریک و کانسنتریک در مردان جوان سالم بود. در یک کارآزمایی میدانی 10 مرد سالم بصورت تصادفی در دو گروه انقباض کانسنتریک و اکسنتریک هر گروه 5 نفر قرار گرفتند. پروتکل­های انقباض آیزوکینتیک شامل اکسنتریک و کانسنتریک  اکستنشن زانو با حداکثر قدرت و سرعت زاویه ای 60 درجه بر ثانیه بود. گشتاورهای تعیین شده برای هر آزمودنی به منظور همسان سازی بارکاری در هر دو پروتکل یکسان در نظر گرفته شد و سرعت رفت و برگشت 60 درجه بر ثانیه بود. در ابتدا و انتهای فعالیت از بافت عضله پهن جانبی برای بررسی بیان ژن MyoD و MRF4 بایوپسی انجام شد. نتایج نشان داد، تغییرات درون گروهی MRF4 بعد از یک جلسه فعالیت، در گروه اکسنتریک (229/0p = ) و کانسنتریک (178/0p = ) معنادار نبود. همچنین تغییرات بین گروهی نشان دهنده عدم تفاوت بین دو گروه بود (182/0p = ). همچنین تغییرات درون­گروهی MyoD بعد از یک جلسه فعالیت، در گروه اکسنتریک (001/0p =) معنادار ولی در گروه کانسنتریک (318/0p = ) معنادار نبود. همچنین تغییرات بین گروهی نشان دهنده عدم تفاوت بین دو گروه بود (821/0p = ). درمجموع مطالعه حاضر نشان داد یک جلسه فعالیت اکسنتریک و کانسنتریک منجر به تغییر فاکتورهای درگیر در قدرت و هایپرتروفی عضلات اسکلتی می­شود. علاوه براین، این تغییرات در مجموع در انقباض اکسنتریک بیش از کانسنتریک بود. بر این اساس توصیه می­شود جهت افزایش هایپرتروفی و قدرت عضلانی تمرکز بیشتری بر تمرینات کانسنتریک شود.

کلیدواژه‌ها


عنوان مقاله [English]

Response of MyoD and MRF4 Myokines to Eccentric and Concentric Activity in Healthy Young Men

نویسندگان [English]

  • Maryam Aqaamini Fashami
  • Hassan Matin Homayi
Department of Physical Education and Sports Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
چکیده [English]

The present study aimed to investigate the response of MyoD and MRF4 myokines to eccentric and concentric activity in healthy young men. Ten healthy males were split into two groups of five, each with concentric and eccentric contractions. Excentric and concentric knee extension with a maximal power and angular velocity of 60 per second were used in isokinetic contraction protocols. The torque set for each subject was considered the same in both protocols to match the load, and the reciprocating speed was 60 degrees per second. At the beginning and end of the activity, the extensive biopsy was performed to examine the expression of the MyoD and MRF4 genes. The results showed that the intragroup changes of MRF4 after one session of activity were not significant in the eccentric (p = 0.229) and concentric (p = 0.178) groups. Furthermore, intergroup changes showed no difference between two groups (p = 0.182). Moreover, the changes within MyoD group after one activity session were significant in the eccentric group (p = 0.001) but not significant in the concentric group (p = 0.318). Moreover, intergroup changes showed no difference between the two groups (p = 0.821). In general, the present study showed that one session of eccentric and concentric activity leads to changes in the factors involved in skeletal muscle strength and hypertrophy. Besides, these changes were generally greater in the eccentric than concentric contraction. Therefore, it is recommended to focus more on concentric exercises to increase hypertrophy and muscle strength.

کلیدواژه‌ها [English]

  • eccentric contraction
  • concentric contraction
  • MyoD
  • MRF4
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