بررسی اثر سوستانون بر بیان ژن گیرنده آندروژن، تغییرات هیستولوژیک بیضه و هورمون های گنادوتروپین در موش صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

2 گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

3 فلوشیپ پاتولوژی مولکولی و سیتوژنتیک، بیمارستان امام رضا (ع)، مرکز ناباروری میلاد، دانشگاه علوم پزشکی مشهد، ایران

چکیده

سوستانون دارویی آنابولیک-آندروژنیک استروئیدی تزریقی مبتنی بر روغن است که شامل چهار استر مختلف تستوسترون است. تستوسترون، آندروژن اصلی در بیضه است که توسط گیرنده آندروژن عمل می­کند. مطالعه حاضر با هدف بررسی بیان ژن AR، تغییرات هیستولوژیک بیضه و هورمون ها ی گنادوتروپین در موش صحرایی پس از استفاده از دوزهای مختلف سوستانون انجام شد. در این مطالعه 24 موش نر بالغ به 4 گروه تقسیم بندی شدند. گروه اول (شاهد) به صورت تزریق عضلانی حلال سوستانون (روغن بادام زمینی) دریافت کردند. به گروه های دوم، سوم و چهارم به ترتیب دوزهای 10، 20 و 30 میلی گرم بر کیلوگرم سوستانون به مدت 8 هفته به صورت هفتگی تزریق شد. یک هفته پس از آخرین تزریق خونگیری به منظور تعیین غلظت هورمون­ها انجام شد. بیضه­ها برای بیان بررسی ژن و مطالعات هیستولوژیک برداشته شدند. Real-Time PCR برای اندازه گیری بیان ژن AR با استفاده از cDNA سنتز شده انجام شد. تجزیه و تحلیل آماری داده­ها توسط نرم­افزار SPSS و آنالیز واریانس انجام شد. با توجه به یافته­های به دست آمده، نشان داده شد که سوستانون تعداد سلول­های لیدیگ و سرتولی را به طور قابل توجهی کاهش می­دهد در نتیجه منجر به کاهش قابل توجه غلظت FSH و LH می­شود. بیان ژن AR وابسته به دوز بود و در مقایسه با گروه کنترل در دوز 10 میلی­گرم بر کیلوگرم افزایش معنادار و در دوز 30 میلی­گرم بر کیلوگرم کاهش معنادار داشت. سوستانون با یک ساختار منحصر به فرد متمایز می­شود. این دارو آسیب­های بیوشیمیایی و هیستولوژیک ایجاد کرده و باعث تغییر در بیان ژن AR را در موش­های صحرایی می­شود که ممکن است باعث ناباروری شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Sustanon on Androgen Receptor Gene Expression, Testicular Histological and Gonadotropin Hormones Changes in Rats

نویسندگان [English]

  • Fatemeh Kamali Hossein Zadeh 1
  • Jina Khayat zadeh 1
  • Mohamad Mahdi Forghani Fard 2
  • Armin Attaran Zadeh 3
1 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2 Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 Molecular Pathology and Cytogenetic Fellowship, Imam Reza Hospital, Milad Infertility Center, Mashhad University of Medical Sciences, Iran
چکیده [English]

Sustanon is considered an oil-based injectable anabolic-androgenic steroid. It comprises four different testosterone esters. The principal androgen in the testis is testosterone which is mediated by the androgen receptor. The current study aimed to investigate AR gene expression and biochemical and histological analyses in male rats after using various dosages of Sustanon. There were four groups of male adult rats. The first group (control) received an intramuscular injection of Sustanon solution (peanut oil). The second, third, and fourth groups underwent the treatment with a weekly injection of different Sustanon dosages of 10, 20, and 30 mg/kg for 8 weeks, respectively. The blood samples were obtained and the measurement of the serum levels of FSH and LH were conducted. The testes were extracted for histopathological and gene expression investigation. Using generated cDNA, real-time PCR was used to evaluate AR gene expression. SPSS software was used to do statistical analysis and analysis of variance on the data. Sustanon was shown to drastically lower the number of Leydig and Sertoli cells, perhaps resulting in a large drop in FSH and LH concentrations, according to the results.The expression of AR was dose-dependent and significantly increased at a dose of 10 mg / kg and decreased significantly at a dose of 30 mg / kg compared to the control group. Sustanon is distinguished by its unique structure. It induced biochemical plus histopathological damages and changed AR gene expression in adult male rats that may cause infertility.

کلیدواژه‌ها [English]

  • Sustanon
  • AR
  • Sertoli
  • Leydig
  • Gonadotropin
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