اثر ضد اضطرابی و بهبود حافظه ناشی از اولئوروپین در مدل حیوانی ایسکمی

نوع مقاله : مقاله پژوهشی

نویسنده

گروه زیست شناسی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

چکیده

ایسکمی مغزی حالتی است که به تمام یا بخش‌هایی از مغز، خون و در نتیجه اکسیژن کافی نمی‌رسد. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺧﺎﺻﻴﺖ آنتی‌اکسیدانی اولئوروپین، مطالعه ﺣﺎﺿﺮ با هدف ﺑﺮرﺳﻲ اﺛﺮ این ماده موثره بر اضطراب و بهبود حافظه در ﻣﺪل حیوانی ایسکمی صورت گرفت. در این تحقیق 50 سر موش صحرایی نژاد ویستار در محدوده‌ی وزنی 200 تا 250 گرم به‌طور تصادفی در 5 گروه شامل :گروه کنترل؛ گروه ایسکمی با انسداد هر دو شریان کاروتید مشترک و سه گروه ایسکمی که اولئوروپین  با دوزهای (10، 50 و100 میلی­گرم بر کیلوگرم) به روش گاواژ به مدت 14روز دریافت کردند تقسیم شدند. برای ایجاد ایسکمی شریان‌های کاروتید عمومی دو طرفه جدا شده، از دو قسمت مسدود و از بخش میانی قطع شدند. ارزیابی حافظه با استفاده از آزمون یادگیری اجتنابی غیر فعال (شاتل باکس) و برای ارزیابی میزان اضطراب از دستگاه ماز بعلاوه ای شکل مرتفع استفاده شد. داده‌ها با استفاده از آزمون‌های تجزیه واریانس یک‌طرفه و پشتیبانی LSD تجزیه و تحلیل شدند. ایسکمی باعث کاهش حافظه (001/0> p). و کاهش اضطراب شد (001/0 > p) و تجویز دوزهای 10، 50، 100 میلی‌گرم در کیلو‌گرم اولئوروپین به ویژه دوز 100 میلی گرم بر کیلوگرم منجر به بهبود نقایص ایجاد شده در حافظه (001/0 > p) و اضطراب (001/0 > p) گردید. براساس نتایج این مطالعه به نظر می‌رسد اولئوروپین به واسطه‌ی خاصیت آنتی اکسیدانی و مهار رادیکال‌های آزاد، از شدت اختلالات شناختی و رفتاری ناشی از ایسکمی هیپوپرفیوژن

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Anxiety and Oleuropein-induced Memory Improvement in an Animal Model of Ischemia

نویسنده [English]

  • Shahrbanoo Alami Rostami
Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran
چکیده [English]

Cerebral ischemia is a condition not reaching all or part of the brain, blood, and therefore oxygen. According to the property oleuropein antioxidant, the current study was performed aiming at using this substance to affect anxiety and improve memory in ischemic animals. In this study, 50 Wistar rats in the weight range of 200 to 250 g were randomly assigned to 5 groups including: control group; the ischemia group was divided by occlusion of both common carotid arteries and the three ischemia groups that received oleuropein at doses (10, 50, and 100 mg/kg) by gavage for 14 days. To cause ischemia, bilateral carotid arteries were isolated, blocked in two, and severed in the middle. Memory assessment was performed using the passive avoidance learning test (shuttle box) and the elevated plus maze device was used to assess anxiety. Data were analyzed using one-way analysis of variance (ANOVA) and LSD support. Ischemia decreased memory (p <0.001). Anxiety was reduced (p <0.001) and administration of doses of 10, 50, 100 mg/kg oleuropein, especially 100 mg/kg, led to improvement in memory deficits (p <0.001) and anxiety (p <0.001). Based on the results of this study, it seems that oleuropein reduces the severity of cognitive and behavioral disorders caused by hypoperfusion ischemia due to its antioxidant properties and inhibition of free radicals.

کلیدواژه‌ها [English]

  • anxiety
  • Memory
  • Oleuropein
  • Cerebral ischemia
  • Male rat
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