تاثیر دوازده هفته مصرف عصاره چای سبز بر بیان ژن Bax و bcl-2 بافت قلب رت‌های مسن در پاسخ به فعالیت حاد وامانده‌ساز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران

2 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه رازی، کرمانشاه، ایران

چکیده

بروز بیماری­های قلبی در اثر پیری به صورت فزاینده­ای افزایش می­یابد. چای سبز یک آنتی اکسیدان است که می­تواند  از پیری سلولی جلوگیری کند. میتوکندری نقشی حیاتی در مسیر داخل سلولی آپوپتوز ایفا می­کند. پروتئین­های Bax و bcl-2 تنظیم کننده­های اصلی آپوپتوز وابسته به میتوکندری هستند. از اینرو هدف این پژوهش بررسی تاثیر 12  هفته مصرف عصاره چای سبز بر بیان ژن Bax و bcl-2 بافت قلب رت­های مسن در پاسخ به فعالیت حاد وامانده ساز بود. 32 سر رت نر مسن به صورت تصادفی در 2 گروه 16 تایی شامل گروه گروه مکمل و گروه بدون مکمل قرار گرفتند. گروه مکمل 12 هفته مکمل عصاره چای سبز دریافت کردند. در انتهای 12 هفته هر کدام از این گروه­ها خود به دو گروه استراحت و تمرین حاد وامانده ساز تقسیم شدند. گروه بدون مکمل همراه با استراحت را گروه کنترل، گروه بدون مکمل همراه با تمرین را گروه تمرین، گروه مکمل همراه با استراحت را گروه مکمل و آخرین گروه را مکمل همراه با  تمرین نام گذاری شدند. بعد از 12 هفته مصرف عصاره چای سبز یک جلسه تمرین وامانده ساز تا حد خستگی بر روی نوارگردان انجام شد. برای اندازه­گیری بیان ژن Bax و bcl-2 از روش Real time PCR استفاده شد. نتایج نشان داد که 12 هفته مصرف عصاره چای سبز موجب کاهش معنی­دار بیان ژن Bax و نسبت بیان ژن Bax به بیان ژن bcl-2 و همچنین افزایش معنی­دار بیان ژن bcl-2 بافت قلب در پاسخ به یک جلسه فعالیت حاد وامانده ساز شد. یک جلسه تمرین وامانده ساز موجب افزایش معنی­دار بیان ژن Bax و نسبت بیان ژن Bax به bcl-2 و همچنین کاهش معنی­دار بیان ژن bcl-2  در بافت قلب شد. نتایج این پژوهش نشان می­دهد که 12 هفته مصرف عصاره چای سبز می­تواند سبب کاهش بیان ژن Bax و نسبت بیان ژن Bax به بیان ژن bcl-2 و افزایش بیان ژن bcl-2 در بافت قلب درحالت استراحت و پس از انجام فعالیت حاد وامانده ساز شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 12 Weeks of Green Tea Extract Consumption on Expression of Bax and bcl-2 Genes of Old ’Rats’ Heart Tissue in Response to Acute Exhaustive Exercise

نویسندگان [English]

  • Ahmad Kazemi 1
  • Naser Behpoor 1 2
  • Ahmad Hematfar 1
1 1- Department of Physical Education, Islamic Azad University, Boroujerd Branch, Boroujerd, Iran
2 Department of Physical Education, Islamic Azad University, Boroujerd Branch, Boroujerd, Iran|Department of Sport Sciences, Razi University, Kermanshah, Iran
چکیده [English]

The incidence of heart disease increases with age. Green tea is an antioxidant that can prevent cellular senescence. Mitochondria play a vital role in the intracellular pathway of apoptosis. Bax and bcl-2 proteins are the main regulators of mitochondrial apoptosis. Thus, the present study is aimed at investigating the effect of 12 weeks of green tea extract consumption on expression Bax and bcl-2 genes of old rats’ heart tissue in response to acute exhaustive exercise. Thirty-two male rats were randomly assigned to two groups of supplement and non-supplement, each consisting of 16 rats. The supplement group received green tea extract for 12 weeks. At the end of the 12 weeks, each of these groups was further divided into two groups of rest and acute exhaustive exercise. The non-supplement with the rest group was called the control group. The non-supplement with exercise group was called exercise group, the supplement group with rest was called the supplement group and finally, the last group was entitled to supplement with exercise. After 12 weeks of taking green tea extract, one session of the acute exhaustive exercise was held on the treadmill. The real-time PCR method was used to measure the expression of Bax and bcl-2 genes. The results showed that 12 weeks of green tea extract consumption significantly reduced Bax gene expression and Bax to bcl-2 gene expression ratio and significantly increased cardiac tissue bcl-2 gene expression in response to acute exhaustive exercise. About acute exhaustive exercise session, it significantly increased Bax gene expression and Bax to bcl-2 gene expression ratio as well as significantly decreasing bcl-2 gene expression in heart tissue. The study concludes that 12 weeks of green tea extract can reduce Bax gene expression and Bax to bcl-2 gene expression ratio and significantly increase cardiac tissue bcl-2 gene expression at rest and after acute exhaustive exercise
 

کلیدواژه‌ها [English]

  • Green tea
  • Exhaustive Exercise
  • Apoptosis
  • BAX
  • Bcl-2
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