بررسی بیان ژنSCAMP5 وmiR هدف آن در بافت بیماران گلیوبلاستوما مولتی‌فرم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

2 گروه زیست فناوری دارویی، دانشگاه علوم پزشکی، زنجان، ایران

3 گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

چکیده

گلیوبلاستومامولتی­فرم (GBM) یکی از بدخیم­ترین و رایج­ترین تومورهای مغزی است و پس از درمان ترکیبی فعلی، بقای بیمار نسبتاً ضعیف است و این بیماری همواره کشنده است. هدف ازاین مطالعه بررسی بیانSCAMP5  به عنوان بیومارکر احتمالی در گلیوبلاستوما مولتی فرم و بررسی miR هدف این ژن می باشد. در این مطالعه 50 نمونه بافت مبتلا به گلیوبلاستوما مولتی فرم قبل از درمان و 50 نمونه بافت سالم حاشیه تومور GBM به عنوان نمونه کنترل جمع­آوری شد. برای تمام نمونه­ها، استخراج RNA و سنتز cDNA  صورت گرفت. بیان ژن SCAMP5 با استفاده از روشReal-time PCR  بررسی شد و از ژنACTB  به عنوان کنترل داخلی استفاده شد. تجزیه وتحلیل آماری داده­ها با استفاده از نرم افزار GraphPad Prism نسخه8  انجام شد. برای بررسی ارزش بیومارکری ژن SCAMP5 از منحنیRoc استفاده شد. کاهش بیان ژن SCAMP5 در نمونه­های گلیوبلاستوما مولتی فرم مشاهده شد و بیان آن با مشخصات پاتولوژی بیماران مقایسه شد. بیان ژن  SCAMP5  با سن بیماران با 05/0  p ˂از لحاظ آماری معنادار بود و بیان ژن با جنسیت با 005/0 p ˃از لحاظ آماری معنادار نمی­باشد. SCAMP5 در بافت توموری بیماران مبتلا بهGBM  به مراتب کمتر از بافت سالم اطراف تومور بیان شده بود. نتایج نشان داد که در بیمارانی با سن بیشتر از 50 سال میزان بیان ژن SCAMP5 کاهش یافت. بررسی منحنی ROC نشان داد که ژن SCAMP5 می­تواند دارای ارزش بیومارکری باشد. با توجه به این بررسی شاید بتوان از بیان این ژن به عنوان مارکر یک راه تشخیصی زودهنگام استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of SCAMP5 Gene Expression and Its Target miR in the Tissue of Patients with Glioblastoma Multiforme

نویسندگان [English]

  • Nasrin Karimi 1
  • Hamidreza Kheiri Manjilli 2
  • Vajiheh Zarrinpour 3
  • Mohammad mahdi Forghanifard 3
1 Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
2 Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
3 Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
چکیده [English]

Glioblastoma multiforme is one of the most malignant and common brain tumors, and after current combination therapy, patient survival is relatively poor and the disease is always fatal. This study was aimed at investigating the expression of SCAMP5 as a possible biomarker in GBM and evaluating the target miR of this gene. 50 tissue samples with GBM form before treatment and 50 healthy tissue samples from the GBM tumor margin were collected as a control. RNA extraction and cDNA synthesis were performed for all samples. Subsequently, a specific primer was designed and the expression of SCAMP5 gene was examined using Real-time PCR. ACTB gene was used as the internal control. Statistical analysis was carried out using Graphpad Prism software version 8. Roc curve was used to evaluate the biomarker value of SCAMP5 gene. Decreased expression of SCAMP5 gene was observed in GBM samples and its expression was compared with the pathological characteristics of patients. Expression of SCAMP5 gene with patients’ age with P-value ˂0.05 was statistically significant. However, the expression of this gene with patients’ gender with P-value ˃ 0.05 was not statistically significant. SCAMP5 was expressed in tumor tissue of patients with GBM much lower than healthy tissue around the tumor. The results revealed that SCAMP5 expression decreased in patients older than 50 years. ROC curve showed that the SCAMP5 gene could have a biomarker value. According to this study, it may be possible to use the expression of this gene as a marker as an early diagnostic method.

کلیدواژه‌ها [English]

  • SCAMP5
  • Glioblastoma multiforme
  • Real-time PCR
  • biomarker
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