بررسی اثرات تزریق کربوهیدرات‌ها و آنتی اکسیدان‌ها در تخم مرغ‌های بارور انبار شده بر جوجه درآوری، ریخت‌شناسی روده، پارامترهای خونی، فعالیت آنتی‌اکسیدانی و محتوای گلیکوژن بافت جوجه‌های تازه بدنیا آمده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه علوم دامی، واحد ورامین-پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران

چکیده

ت
تحقیق حاضر جهت بررسی اثر تغذیه درون تخم­مرغی کربوهیدرات‌ها و آنتی­اکسیدان‌ها در تخم­مرغ­های جوجه­کشی انبارشده انجام شد. برای انجام آزمایش 1200 عدد تخم­مرغ قابل جوجه­کشی از مرغ مادر گوشتی سویه کاب 500 پس از انبار شدن در دو بازه زمانی 3 و 14 روز جهت تزریق آمنیونی در روز 5/17 جنینی، در قالب طرح کاملا تصادفی به 10 تیمار و 5 تکرار (هر تکرار 24 عدد تخم مرغ) توزیع شدند. تیمارهای آزمایشی شامل: 1) کنترل منفی (بدون تزریق)، 2) کنترل مثبت  (تزریق 5/0 میلیلیتر محلول نرمال سالین)، 3) تزریق 5/0 میلی­لیتر محلول قندی، 4) تزریق 5/0 میلی­لیتر محلول آنتی اکسیدان و 5) تزریق 5/0 میلی­لیتر مخلوط محلول قندی و آنتی­اکسیدان بودند. درصد تلفات در تیمارهای مخلوط کربوهیدرات­ها و آنتی­اکسیدان­ها در هر دو بازه زمانی کمتر از شاهد شد (05/0 > p). عمق کریپت و نسبت طول به عرض پرز روده و همچنین گلیکوژن ماهیچه ران جوجه­هایی که از تخم­مرغ­های تحت تزریق کربوهیدرات­ها و مخلوط کربوهیدات­ها و آنتی­اکسیدان­ها  در طی دو بازه زمانی 3 و 14 روز انبارداری که بدنیا آمده بودند، بهبود معنی­داری نسبت به شاهد داشتند (05/0 > p). غلظت گلوکز خون جوجه­هایی که از تخم­مرغ­های تحت تزریق کربوهیدرات­ها، آنتی­اکسیدان­ها و مخلوط آن­ها و 3 روز انبارداری بدنیا آمده بودند، افزایش معنی­داری نسبت به شاهد داشت (05/0 > p). ظرفیت آنتی­اکسیدانی کل در جوجه­هایی که از تخم­مرغ­های تحت تزریق آنتی­اکسیدان­ها و 3 روز انبارداری بدنیا آمده بودند، افزایش معنی­داری نسبت به شاهد داشت (05/0 > p). نتایج نشان می­دهد که تزریق کربوهیدرات­ها، آنتی­اکسیدان­ها و مخلوط کربوهیدرات­ها و انتی­اکسیدان­ها در دوران قبل از هچ در تخم­مرغ­های بارور ذخیره شده، موثر می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of the in ovo Injection of Carbohydrates and Antioxidants into the Stored Incubating Eggs on Hatchability, Intestinal Morphology, Blood Parameters, Antioxidant Activity and Tissue Glycogen Store of Newly Hatched Chicks

نویسندگان [English]

  • Mohammad Naeim Asa 1
  • Mohammad Chamani 1
  • Seyed Naser Mousavi 2
  • Ali-Asghar Sadeghi 1
  • Farhad Foroudi 2
1 Department of Animal Science, Islamic Azad University, Science and Research Branch, Tehran, Iran
2 Department of Animal Science, Islamic Azad University, Varamin-Pishva Branch, Varamin, Iran
چکیده [English]

The present study was conducted to investigate the effect of in ovo feeding of carbohydrates and antioxidants in the stored incubating eggs. To perform this experiment, 1200 hatching eggs from the broiler hens of Cob 500 strain were distributed after storage in two time intervals of 3 and 14 days for amniotic injection on the 17.5th embryonic day in a completely randomized design with 10 treatments and 5 iterations (each iteration with 24 eggs). The experimental treatments include: 1) negative control (without injection), 2) positive control (injection of 0.5 ml normal saline solution), 3) injection of 0.5 ml carbohydrate solution, 4) injection of 0.5 ml antioxidant solution and 5) injection of 0.5 ml of a mixture of carbohydrate and antioxidant solution. The percentage of losses in the treatments of mixed carbohydrates and antioxidants in both periods was less than the control (P<0.05). Crypt depth and length-to-width ratio of intestinal villi, as well as the glycogen in the thigh muscle of the chickens hatched from the eggs injected with carbohydrates, a mixture of carbohydrates and antioxidants during two storage periods of 3 and 14 days, had a significant improvement compared to the control (P<0.05). Blood glucose concentration of the chickens hatched from the eggs injected with carbohydrates, antioxidants, and their mixtures with 3 days of storage had a significant increase compared to the control (P<0.05). Total antioxidant capacity in the chickens hatched from the eggs injected with antioxidants and 3 days of storage had a significant increase compared to the control (P<0.05). The results show that the injection of carbohydrates, antioxidants, and a mixture of carbohydrates and antioxidants in the pre-hatching period would be effective on the stored fertile eggs.

کلیدواژه‌ها [English]

  • Antioxidant
  • chick
  • hatchability
  • Morphology
  • Carbohydrate
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