بررسی تجمع فلزات سنگین سرب و کادمیوم در بافت‌های آبشش، پوست و عضله Chalcalburnus Chalcoides (تالاب انزلی و شیرود)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه بیولوژی دریا، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

تحقیق حاضر، به منظور تعیین غلظت فلزات سنگین سرب و کادمیوم در بافت‌های آبشش، پوست و عضله ماهی شاه کولی صید­شده از آبهای حوضه جنوبی دریای خزر (تالاب انزلی و شیرود) در استان مازندران وگیلان صورت گرفت. بافت نمونه­ها پس از انجام زیست­سنجی، تفکیک شدند. برای استخراج فلزات از بافت­ها از روش هضم شیمیایی توسط اسید نیتریک استفاده گردید و غلظت فلزات توسط دستگاه جذب اتمی کوره تعیین گردید. میانگین غلظت فلزات (بر حسب ppm وزن خشک) با استاندارد­های جهانی نظیر استاندارد جهانیWHO،FAO  و  Stoskopfدر مطالعه مقایسه شد. بر اساس نتایج به دست آمده، در منطقه شیرود از استان مازندران حداکثر میانگین تجمع فلزات در بافت‌های آبشش، پوست و عضله متعلق به سرب 52069/2 ± 5836/9، 08995/1 ± 9343/5 و 45947/1 ± 0264/6 میکروگرم بر گرم و حداقل میانگین تجمع فلزات در بافت‌های آبشش، پوست و عضله متعلق به کادمیوم 04763/0 ± 0936/0، 04236/0 ± 0957/0 و 02227/0 ± 0393/0 میکروگرم بر گرم می­باشد. در منطقه تالاب انزلی از استان گیلان حداکثر میانگین تجمع فلزات در بافت‌های آبشش، پوست و عضله متعلق به سرب 68153/3 ± 8380/20، 04173/2 ± 6880/7 و 68273/0 ± 5653/3 میکروگرم بر گرم و حداقل میانگین تجمع فلزات در بافت‌های آبشش، پوست و عضله متعلق به کادمیوم 22663/0 ± 6040/0، 03897/0 ± 1167/0 و 00893/0 ± 0233/0 میکروگرم بر گرم می­باشد. غلظت سرب حاضر در هر دو منطقه در مقایسه با استاندارد­های جهانی WHO، Netherlands،EOS  و EPA بالاتر از حد­مجاز به دست آمد، به جز عضله در استان گیلان که براساس استانداردEPA  پایین­تر از حد ­مجاز است غلظت کادمیوم براساس استاندارد­ها در تمامی بافت­ها به­جز آبشش پایین­تر است. همچنین میانگین غلظت فلزات سرب و کادمیوم بین بافت‌های عضله، پوست وآبشش در استان گیلان تفاوت معنی­داری نشان داد (05/0 > p). غلظت فلزات سرب وکادمیوم در بافت‌های فوق در استان مازندران تفاوت معنی­داری نشان نداد (05/0 > p).

کلیدواژه‌ها


عنوان مقاله [English]

Study of Accumulation of Heavy Metals (Pb & Cd) in Muscle, Skin, and Gill Tissues Chalcalburnus Chalcoides (Anzali & Shirud Lagoon)

نویسندگان [English]

  • Mohammad Bostanmanesh
  • Mahnaz Sadat Sadeghi
  • Mojgan Emtiyazjoo
Department of Marine Biology, Islamic Azad University, North Tehran Branch, Tehran, Iran
چکیده [English]

We studied concentration of heavy metals Pb and Cd in the Gill, Skin, and Muscle tissues of Chalcalburnus Chalcoides caught from the southern Caspian Sea (Anzali & Shirud lagoon) in spring 2012. After biometrical measurements, the Gill, Skin, and Muscle tissues of samples were separated; metals were extracted from the tissues using chemical digestion method with pure nitric acid and their concentrations were determined by AAS. Average concentrations (in ppm dry weight) were compared with international standards such as FAO, WHO, and Stoskopf. Results showed that in Shirud of Mazandaran in gills, skin and muscle tissues, the maximum average accumulation belonging to Pb are 9.5836 ± 2.52069, 5.9343 ± 1.08995, 6.0264 ± 1.45947 µg/g and minimum average belonging to Cd are 0.0936 ± 0.04763, 0.0957 ± 0.04236, 0.0393 ± 0.02227 µg/g, respectively. In Anzali of Gilan, in gills, skin, and muscle tissues maximum average accumulation belonging to Pb are 20.8380 ± 3.68153, 7.6880 ± 2.04173, 3.5653 ± 0.68273 µg/g and minimum average belonging to Cd are 0.6040 ± 0.22663, 0.1167 ± 0.03897, 0.0233 ± 0.00893 µg/g, respectively. Lead concentrations in this study in both areas compared to international standards of WHO, Netherlands, EOS, and EPA limit is reached, but according to the EPA standard, the muscles in Gilan province are lower than the limit. Cadmium concentrations in all tissues except gill-based standards are lower. The average concentration of lead and cadmium metals between muscle, skin, and gills in Gilan province showed significant difference (P<0.05) and average concentrations of lead and cadmium in the tissues in Mazandaran province were not significantly different (P> 0.5).
 

کلیدواژه‌ها [English]

  • Heavy metals
  • Chalcalburnus chalcoides
  • Caspian Sea
  • Gilan and Mazandaran Provinces
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