بررسی اثرات مت آمفتامین بر روی ترکیبات کیسه آمنیوتیک در موش های باردار و تخمدان فرزندان آنها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

3 گروه زیست شناسی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

چکیده

مت­آمفتامین نوعی داروی محرک سیستم عصبی مرکزی است که مصرف آن در سال­های اخیر در میان نوجوانان و جوانان بسیار رواج یافته است. از آنجا که استفاده از مواد مخدر از جمله مت آمفتامین می­تواند تأثیر بالقوه­ای بر روی مایع آمنیوتیک، بیان ژن های مؤثر در مادر و جنین، بافت­ها، هورمون­ها و سایر فاکتورها داشته باشد، لذا هدف از پژوهش حاضر بررسی اثرات مت آمفتامین بر کیسه آمنیون موش­های باردار و تأثیرات آن بر نسل های بعد آن نیز می­باشد. در این مطالعه، 60 سر موش صحرایی بالغ ماده نژاد ویستار به شش گروه تقسیم شدند: گروه کنترل، گروه شم، گروه دریافت کننده دوز یک میلی­گرم بر کیلوگرم آمفتامین، گروه دریافت کننده دوز 2 میلی­گرم بر کیلوگرم آمفتامین، گروه دریافت کننده دوز 3 میلی­گرم بر کیلوگرم آمفتامین و گروه دریافت کننده دوز 4 میلی­گرم بر کیلوگرم آمفتامین. پس از پایان دوره آزمایش، خونگیری از قلب موش­ها انجام شد و پارامترهای مورد نظر (تستوسترون، گلوکز، LH، FSH،AST ، ALT، WBC وRBC ) اندازه­گیری شدند. سپس موش­ها تشریح، مایع آمنیوتیک به منظور بررسی میزان پروتئین، کربوهیدرات­ها، چربی­ها، فسفولیپید و اوره جدا شد. تزریق درون صفاقی مت آمفتامین تغییرات قابل توجهی را در میزان پرامترهای بیوشیمیایی اندازه­گیری شده همچون LH،  T.Gو تستوسترون نسبت به گروه کنترل ایجاد نمود. علاوه­ براین مت آمفتامین بر روی FSH تغییری را ایجاد نکرد. از طرفی با افزایش دوز مت آمفتامین، بیان ژن‌های HOX و TUBB8 کاهش یافت. نتایج این مطالعه نشان می دهد که  تزریق مت­آمفتامین حتی در دوزهای پایین، باعث افزایش یا کاهش فعالیت تخمدان و اووژنز و در نتیجه اختلال در کیفیت تخمک گذاری و بلوغ اووسیت­ها می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Effects of Methamphetamine on Amniotic Sac Composition in Pregnant Mice and Their Offspring

نویسندگان [English]

  • Milad Rezazadeh 1
  • Ramin Hajikhani 1
  • Gholamhassan Vaezi 2
  • Maryam Bananej 3
1 Department of Biology, Islamic Azad University, North Tehran Branch, Tehran, Iran
2 Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
3 Department of Biology, Islamic Azad University, Karaj Branch, Karaj, Iran
چکیده [English]

Methamphetamine is a central nervous system stimulant drug that has become very popular among adolescents and young people in recent years. Since the use of drugs including methamphetamine can have a potential effect on amniotic fluid, expression of genes can affect mother and fetus, tissues, hormones, and other factors, this study was aimed at investigating the effects of methamphetamine on the amniotic sac of pregnant rat and its effects on subsequent generations. In this study, 60 adult Wistar female rats were assigned to six groups: control group, sham group, group receiving 1 mg/kg amphetamine, group receiving 2 mg/kg amphetamine, group receiving 3 mg/kg amphetamine, and group receiving 4 mg/kg amphetamine. At the end of the experimental period, blood samples were taken from the hearts of mice and the desired parameters (testosterone, glucose, LH, FSH, AST, ALT, WBC, and RBC) were measured. The rats were then dissected; amniotic fluid was isolated to evaluate the amount of protein, carbohydrates, fats, phospholipids, and urea. Intraperitoneal injection of methamphetamine caused significant changes in the amount of biochemical parameters measured such as LH, T.G, and testosterone compared to the control group. In addition, methamphetamine did not alter FSH. With increasing methamphetamine dose, expression of HOX and TUBB8 genes decreased. The results of this study show that even at low doses, methamphetamine injection increases or decreases ovarian activity and ovogenesis, resulting in impaired ovulation quality and oocyte maturation.

کلیدواژه‌ها [English]

  • Methamphetamine
  • Amniotic Fluid
  • Ovary
  • Hematoxylin – Eosin
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