اثر تعاملی دویدن در شیب های مختلف همراه با مصرف ژل رویال بر غلظت برخی آنتی اکسیدان ها در مدل حیوانی آلزایمری شده با تری متیل تین

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

چکیده

اگرچه نقش تمرینات ورزشی و ژل رویال بر سلامت بیماران مبتلا به آلزایمر گزارش شده است اما اثر تعاملی این دو متغیر بر سوپراکسید دسموتاز (SOD) و گلوتاتیون پراکسیداز (GPx) هنوز به خوبی شناخته نشده است. از این­رو مطالعه حاضر با هدف بررسی اثر تعاملی دویدن در شیب مثبت (PSR) و منفی (NSR) همراه با ژل رویال بر غلظت SOD و GPx در یک مدل حیوانی آلزایمری شده با تری­متیل­تین (TMT) انجام شد. در این کارآزمایی تجربی تعداد 49 سر موش صحرایی در محدوده سنی 8-10 هفته، وزن 190-220 گرم آلزایمری شده با 8 میلی­گرم بر کیلوگرم TMT به هفت گروه شامل گروه­ یک: TMT، گروه دوم: شم، گروه سوم: ژل رویال، گروه چهارم: شیب مثبت، گروه پنجم: شیب منفی، گروه ششم: ژل رویال + شیب مثبت و گروه هفتم: ژل رویال  + شیب منفی تقسیم شدند. همچنین جهت بررسی اثر القا آلزایمر بر متغیرهای تحقیق تعداد هفت سر موش صحرایی سالم در گروه کنترل سالم قرار گرفتند. در ادامه تمرینات شیب مثبت و شیب منفی به مدت هشت هفته، سه جلسه در هفته و هر جلسه 60 دقیقه در شیب مثبت 15 و منفی 15 بر نوار گردان دویدند. ژل رویال نیز روزانه 100 میلی­گرم بر کیلوگرم به صورت صفاقی به موش­های صحرایی تزریق گردید. جهت تجزیه و تحلیل داده­ها از آزمون آنالیز واریانس یک­راهه همراه با آزمون تعقیبی توکی و آزمون تحلیل واریانس دوراهه همراه با آزمون تعقیبی بونفرونی در نرم افزار SPSS 22 استفاده شد (05/0p ≤ ). شیب منفی موجب افزایش SOD و GPx در بافت هیپوکامپ موش­های صحرایی مبتلا به AD گردید. شیب مثبت و ژل رویال موجب افزایش GPx گردیدند. گروه­های شیب مثبت و ژل رویال و شیب منفی و ژل رویال اثر معنی­داری بر افزایش SOD و GPx داشتند. به نظر می­رسد شیب مثبت و منفی و ژل رویال موجب بهبود دفاع آنتی­اکسیدانی می­گردند؛ همچنین تعامل شیب مثبت و ژل رویال و تعامل شیب منفی و ژل رویال مطلوب­تر از اثر هر کدام به تنهایی است.

کلیدواژه‌ها


عنوان مقاله [English]

The Interactive Effect of Running on Different Slopes with the Use of Royal Jelly on the Concentration of Some Antioxidants in an Animal Model of Trimethyltin-treated Alzheimer’s

نویسندگان [English]

  • Hamid Mousazadeh
  • Nematollah Nemati
  • Tahereh Bagherpoor
Department of Sport Science, Islamic Azad University,Damghan Branch, DamghanDepartment of Sport Science, Islamic Azad University,Damghan Branch, Damghan, Iran, Iran
چکیده [English]

Although the role of exercise and royal jelly in the health of Alzheimer’s patients has been reported, the interaction effect of these two variables on superoxide dismutase (SOD) and glutathione peroxidase (GPx) is still not well understood. Therefore, the present study was aimed at investigating the interactive effect of positive slope running (PSR) and negative slope running (NSR) with royal jelly on SOD and GPx concentrations in a trimethyltin (TMT) Alzheimer’s animal model. In this experimental trial, 49 rats in the age range of 8-10 weeks, weighing 190-220 g, Alzheimer’s with 8 mg/kg TMT were assigned into seven groups including group one: TMT, group two: sham, group three: Royal jelly, group four: positive slope, group five: negative slope, group six: royal jelly + positive slope and group seven: Royal jelly + negative slope. Moreover, to investigate the effect of Alzheimer’s induction on the research variables, seven healthy rats were included in the healthy control group following the positive slope and negative slope exercises for eight weeks, three sessions per week and each session ran for 60 minutes on a positive 15 and negative 15 slope on the treadmill. Besides, 100 mg/kg of royal jelly was peripherally injected into rats daily. One-way analysis of variance (ANOVA) with Tukey post hoc test and two-way ANOVA with Bonferroni post hoc test in SPSS 22 software were used to analyze the data (p ≤ 0.05). Negative slope increased SOD and GPx in hippocampal tissue of AD rats. PSR and royal jelly increased GPx. Positive slope and royal jelly and negative slope and royal jelly groups had a significant effect on increasing SOD and GPx. Positive and negative slopes and royal jelly seem to improve antioxidant defense; furthermore, the interaction of positive slope and royal jelly and the interaction of negative slope and royal jelly are more desirable than the effect of each alone.

کلیدواژه‌ها [English]

  • Exercise
  • royal jelly
  • Carbonyl Protein
  • Pain Tolerance Threshold
  • Alzheimer
  1. Ali A.M., Kunugi H. 2020. Royal Jelly as an Intelligent Anti-Aging Agent—A Focus on Cognitive Aging and Alzheimer’s Disease: A Review. Antioxidants, 9(10): 937.‏
  2. Azimpour M., Fathi M., Dezfoulian O. 2021. The Effect of Eight Weeks of Voluntary Wheel Running Exercise with Royal Jelly Consumption on Behavioral Disorders and Antioxidant Capacity in Rats with Trimethytin Model of Alzheimer’s Disease. Journal of Fasa University of Medical Sciences, 11(1): 3702-3714.‏
  3. Deh-Bozorgi A., Behboudi L., Hosseini S. A., Haj-Rasoli M. 2020. Effect of voluntary and forced training with royal jelly consumption on learning and spatial memory of rat model of alzheimer’s disease. Jundishapur Journal of Chronic Disease Care, 9(1): 1-7.‏
  4. Dos Santos J.R., Bortolanza M., Ferrari G. D., Lanfredi G.P., do Nascimento G.C., Azzolini, A.E.C.S., Alberici L.C. 2020. One-week high-intensity interval training increases hippocampal plasticity and mitochondrial content without changes in redox state. Antioxidants, 9(5): 445.‏
  5. Fracassi A., Marcatti M., Zolochevska O., Tabor N., Woltjer R., Moreno S., Taglialatela G. 2021. Oxidative damage and Antioxidant response in frontal cortex of demented and nondemented individuals with Alzheimer's neuropathology. Journal of Neuroscience, 41(3): 538-554.‏
  6. Frye B.M., Craft S., Latimer C.S., Keene C.D., Montine T.J., Register T.C., Shively C.A. 2021. Aging‐related Alzheimer's disease‐like neuropathology and functional decline in captive vervet monkeys (Chlorocebus aethiops sabaeus). American Journal of Primatology, 2021: e23260.
  7. Giti Z., Banaeifar A., Arshadi S., Azarbayjani M.A. 2021. Effect of Eight Weeks of Positive Slope and Negative Slope Training, along with royal jelly on the hippocampal expression of О-amyloid and О-secretase in trimethyltin-induced Alzheimer's disease rats. Journal of Nutrition, Fasting and Health, 9(1): 29-34.
8.      Hassanlouei F., Behbudi Tabrizi L., Haji Rasoli M. 2021. Effect of Endurance Training on Positive Slopes with Royal Jelly Consumption on Dopamine in the Hippocampus Tissue of Rats with Alzheimer's Disease. Journal of Nutrition, Fasting and Health, 9(2): 131-136.
  1. Hosseini S.A., Salehi, O. R., Farzanegi, P., Farkhaie F., Darvishpour A.R., Roozegar S. 2020. Interactive effects of endurance training and royal jelly consumption on motor balance and pain threshold in animal model of the alzheimer disease. Archives of Neuroscience, 7(2): ‏
  2. Hosseini S.A., Slehi O.R., Keikhosravi F., Hassanpour G., Ardakani H.D., Farkhaie F., Azarbayjani M.A. 2021. Mental health benefits of exercise and genistein in elderly rats. Experimental Aging Research, 2021: 1-16.‏
  3. Misrani A., Tabassum S., Yang L. 2021. Mitochondrial dysfunction and oxidative stress in Alzheimer’s disease. Frontiers in Aging Neuroscience, 13: 617588.‏
  4. Noura M., Arshadi S., Zafari A., Banaeyfar A. 2020. Effect of endurance training with royal jelly on crp gene expression in muscle tissue of rats with alzheimer’s disease. Middle East Journal of Rehabilitation and Health Studies, 7(1): e99754.‏
  5. Noura, M., Arshadi, S., Zafari, A., Banaeifar A. 2020. The effect of running on positive and negative slopes on TNF-α and INF-γ gene expression in the muscle tissue of rats with Alzheimer’s disease. Journal of Basic Research in Medical Sciences, 7(1): 35-42.‏
  6. Quan H., Koltai E., Suzuki K., Aguiar Jr, A. S., Pinho R., Boldogh I., Radak Z. 2020. Exercise, redox system and neurodegenerative diseases. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1866(10): 165778.‏
  7. Rahmani A, Gorzi A, Ghanbari M. 2019.The effects of high intensity interval training and strenuous resistance training on hippocampal antioxidant capacity and serum levels of malondialdehyde and total antioxidant capacity in male rats. Journal of Kurdistan Uniersity of Meical Sciences, 23(6): 47-58.
  8. Ramadan M.F., Al-Ghamdi A. 2012. Bioactive compounds and health-promoting properties of royal jelly: A review. Journal of Functional Foods, 4(1): 39-52.‏
  9. Rami M, Habibi A, khajehlandi M. 2018. Effect of 6-weeks of endurance training on the activity of superoxide dismutase and glutathione peroxidase enzymes in the hippocampus of experimental diabetic male Wistar rats. Journal of Shahid Sadoughi University of Medical Sciences, 26(6): 483-494.
  10. Salisbury D., Plocher T., Yu F. 2021. Efficacy of simultaneous aerobic exercise and cognitive training in subjective cognitive decline: study protocol for randomized controlled trial of the exergames Study. Trials, 22(1): 1-10.‏
  11. Shenas N.P., Salehi O., Hosseini S.A. 2021. The Effect of Resistance Training with Royal Jelly on Serotonin and Dopamine Receptors Genes Expression in the Hippocampus of a Rat Model of Alzheimer's Disease, 2021: 10.21203/rs.3.rs-350557/v1.
  12. Silva T.G.S.E., da Silva J.R.M., da Silva Alves A., Britto L.R.G., Xavier G.F., Sandoval, M.R.L. 2020. Oral treatment with royal jelly improves memory and presents neuroprotective effects on icv-STZ rat model of sporadic Alzheimer's disease. Heliyon, 6(2): e03281.‏
  13. Tauffenberger A., Magistretti P.J. 2021. Reactive oxygen species: beyond their reactive behavior. Neurochemical Research, 46: 77-87.‏
  14. Teixeira R.R., de Souza A.V., Peixoto L.G., Machado H.L., Caixeta D.C., Vilela D.D., Espindola F.S. 2017. Royal jelly decreases corticosterone levels and improves the brain antioxidant system in restraint and cold stressed rats. Neuroscience Letters, 655: 179-185.‏
  15. Thirupathi A., Wang M., Lin J.K., Fekete G., István B., Baker J.S., Gu Y. 2021. Effect of Different exercise modalities on oxidative stress: A systematic review. BioMed Research International, 2021: 1947928.‏
  16. Yuede C.M., Timson B.F., Hettinger J.C., Yuede K.M., Edwards H.M., Lawson J.E., Cirrito J.R. 2018. Interactions between stress and physical activity on Alzheimer's disease pathology. Neurobiology of Stress, 8: 158-171.‏