بررسی اثر اسید آسکوربیک و هیدرو کسی آنالوگ متیونین بر عملکرد و بافت‌شناسی کبد جوجه‌های گوشتی تحت شرایط تنش گرمایی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

چکیده

تحقیق حاضر با هدف بررسی اثر اسید آسکوربیک و هیدرو کسی آنالوگ متیونین بر عملکرد و بافت‌شناسی کبد جوجه‌های گوشتی تحت شرایط تنش گرمایی انجام شد. جهت انجام ازمایش از 400 قطعه جوجه نر یکروزه سویه تجاری راس 308 در قالب یک طرح کاملا تصادفی با 4 تیمار و 5 تکرار (هر تکرار حاوی 20 قطعه جوجه) استفاده شد. تیمار های آزمایشی عبارت بودند از: 1)  جیره پایه بر اساس ذرت-سویا (شاهد)، 2) جیره پایه به همراه 2/0 درصد ویتامین C، 3) جیره پایه به همراه 46/0، 36/0 و 32/0 درصد متیونین هیدروکسی آنالوگ به ترتیب در دوره اغازین، رشد و پایانی و 4) جیره پایه به همراه مخلوط  2/0 درصد ویتامین C و 46/0، 36/0 و 32/0 درصد متیونین هیدروکسی آنالوگ به ترتیب در دوره اغازین، رشد و پایانی. در دوره رشد خوراک مصرفی در تیمار حاوی متیونین هیدروکسی آنالوگ افزایش معنی­داری با شاهد داشت (05/0 > p). در دوره پایانی ضریب تبدیل خوراک در تیمارهایی که از مخلوط ویتامین C و متیونین هیدروکسی آنالوگ استفاده کرده بودند بهبود معنی­داری با شاهد داشتند (05/0 >p ). قطر هپاتوسیت و قطر هسته هپاتوسیت در جوجه­هایی که از ویتامین C و مخلوط ویتامین C و متیونین هیدروکسی آنالوگ استفاده کرده بودند کاهش معنی­داری با شاهد داشتند (05/0>P). نتایج این آزمایش نشان داد که بکار بردن مخلوط ویتامین C و متیونین هیدروکسی آنالوگ نسبت به استفاده جداگانه از آن­ها در جیره غذایی می­تواند بر کاهش آثار منفی تنش حرارتی موثر باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Effect of Ascorbic Acid and Methionine Hydroxy Analogue on Performance and Liver Histology of Broilers under Heat Stress Conditions

نویسندگان [English]

  • Mahsa Erfani
  • Nima Eila
  • Abolfazl Zarei
  • Alireza Noshary
Department of Animal Science, Islamic Azad University, Karaj Branch, Karaj, Iran
چکیده [English]

The present study was aimed at investigating the effect of ascorbic acid and methionine hydroxy analogue (MHA) on performance and liver histology of broilers under heat stress conditions. To perform the experiment, 400 one-day-old male chickens of commercial Ross 308 strain were used in a completely randomized design with four treatments and five iterations (each iteration contained 20 chickens). The experimental treatments included: 1) base diet based on maize-soybean (the control), 2) base diet with 0.2% vitamin C, 3) base diet with 0.46, 0.36, and 0.32% MHA, in the starter, grower, and finisher periods, respectively, and 4) base diet with a mixture of 0.2% vitamin C and 0.46, 0.36, and 0.32% of MHA in the starter, grower, and finisher periods, respectively. During the growth period, feed consumption in the treatment containing MHA had a significant increase compared to the control group (P <0.05). In the final period, feed conversion ratio in treatments using a mixture of vitamin C and MHA had a significant improvement compared to the control group (P <0.05). Hepatocyte diameter and hepatocyte nucleus diameter were significantly reduced in chickens receiving vitamin C and a mixture of vitamin C and MHA compared to the control group (P <0.05). The results of this experiment revealed that the use of a mixture of vitamin C and MHA compared to their separate use in the diet could be effective in reducing the negative effects of heat stress.
 

کلیدواژه‌ها [English]

  • liver
  • Ascorbic acid
  • heat stress
  • Methionine Hydroxy Analogue
  • broiler chicken
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