بررسی اثر عصاره آبی گل گندم طلایی (Centaurea cyanus) بر میزان فعالیت آنزیم‌های کبدی (ALT، ASTو ALP) به دنبال مسمومیت کبدی ناشی از مصرف استامینوفن در موش‌های صحرایی نر

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه زیست شناسی، واحد فلاورجان، دانشگاه آزاداسلامی، اصفهان، ایران

چکیده

این پژوهش باهدف بررسی ااثر عصاره آبی گل گندم طلایی بر روی آسیب بافت کبدی ناشی از استامینوفن برروی موش­های صحرایی نرانجام شد. 42 سر رَت نربه طور تصادفی به 7 گروه 6 تایی (گروه شاهد و 5 گروه تیمار) تقسیم شدند و شب قبل از آزمایش به مدت 16-12 ساعت گرسته نگه داشته شده و تمام گروه­ها به مدت 5 هفته هر روز میزان 600 میلی­گرم/ کیلوگرم استامینوفن را به روش خوراکی دریافت کردند.طی مدت آزمایش بر اساس وزن موش­ها میزان 2/0 میلی­لیتر از عصاره گل گندم با غلظت­های 160، 180، 200، 220، 240 و 260 میلی­گرم برکیلوگرم از راه خوراکی و یک ساعت بعد از دریافت دوز سمی استامینوفن دریافت نمودند. آنزیم­های کبدی (AST، ALT، ALP) مورد سنجش و بافت کبد موش­های مورد تیمار توسط میکروسکوپ نوری مورد مطالعه قرار گرفت. یافته های حاصل ازاین پژوهش نشان داد عصاره آبی گل گندم طلایی بر فعالیت آنزیم آسپارتات آمینوترانسفراز اثر گذاشته و در دوز 260 نسبت به سایر گروه­ها بطور معنی دار (05/0 > p) کاهش یافته است. همچنین شدت آسیب­های کبدی اختلاف معنی­داری را بین گروه­ها نشان داد، ولی میزان آلکالان فسفاتاز و آلانین آمینوترانسفراز اختلاف معنی­داری را بین گروه­های مختلف نشان نمی دهد.نتایج حاصل از پژوهش تاثیر عصاره آبی گل گندم طلایی در جلوگیری از بروز آسیب به بافت کبدی به دنبال مسمومیت کبدی ناشی از مصرف استامینوفن را اثبات کرد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Aqueous Extract of Golden Wheat Flower on the Activity of Liver Enzymes (ALT, AST, and ALP) Following Hepatotoxicity Caused by Acetaminophen in Male Rats

نویسندگان [English]

  • Nahid Jahanbazi
  • Shahla Roozbehani
Department of Biology, Flowerjan Branch, Islamic Azad University, Isfahan, Iran
چکیده [English]

This study was aimed at investigating the effect of aqueous extract of golden wheat flower on acetaminophen-induced liver tissue damage in rats. Forty-two male rats were randomly assigned to seven groups of six (control group and five treatment groups) and kept hungry for 12-16 hours the night before the experiment and all groups were given 600 mg/kg daily for five weeks. Acetaminophen was administered orally. During the experiment, based on the weight of mice, 0.2 ml of wheat flower extract with concentrations of 160, 180, 200, 220, 240, and 260 mg/kg orally and one hour after receiving a toxic dose of acetaminophen. Liver enzymes (AST, ALT, and ALP) were assayed and the liver tissue of mice treated was examined under a light microscope. The results of this study showed that the aqueous extract of golden wheat flower affected the activity of aspartate aminotransferase enzyme and at a dose of 260 significantly decreased (p <0.05) compared to other groups. Besides, the severity of liver damage showed a significant difference between the groups; however, the levels of alkaline phosphatase and alanine aminotransferase did not show a significant difference between different groups. The research results proved the effect of the aqueous extract of golden wheat flower in preventing liver tissue damage following liver poisoning caused by taking acetaminophen.

کلیدواژه‌ها [English]

  • Golden Wheat Flower
  • Liver Poisoning
  • Asparate Aminotransferase
  • alkaline phosphatase
  • Alanine aminotransferase
  • Acetaminophen
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