بررسی مقایسه ای عصاره هیپوفیز، LHRH و تیروکسین بر استروئیدهای جنسی، هیستوشیمی بافت تخمدان و اسیدهای چرب مولد مولد ماده روهو (Labeo rohita)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران

چکیده

این مطالعه با هدف بررسی اثر مقایسه­ای هورمون­های هیپوفیز، LHRH و تیروکسین بر شاخص­های هورمونی و اسیدهای چرب تخمدان ماهی مولد ماده روهو (Labeo rohita) انجام شد. هورمون تیروکسین با دوزهای 2، 10 و 50 میکروگرم به ازای هر گرم وزن بدن ماهی مولد در یک تزریق در نظر گرفته شد. در زمان بین تزریق اول و دوم، بعد از تزریق دوم و بعد از تخم­کشی سطح هورمون­های استرادیول، تستوسترون، 17- آلفا هیدروکسی پروژسترون، کورتیزول و LH بالاترین سطح خود را به ترتیب در هیپوفیز و تیمار دریافت کننده دوز 50 میکروگرم وزن بدن و کمترین میزان خود را در تیمارهای دریافت کننده LHRH و شاهد داشتند (05/0p <). در بین تیمارهای دریافت کننده تیروکسین، افزایش سطح تیروکسین توانست هورمون­های محرک فرآیند رسیدگی را به شکل معنی­داری افزایش دهد. تیمار دریافت کننده هیپوفیز و تیمار تیروکسین دریافت کننده 50 میکروگرم با مقدار 70/16 و 99/15 درصد وزن خشک بالاترین میزان پروتئین تخمدان و دو تیمار LHRH و شاهد کمترین سطح پروتئین تخمدان را داشتند. تیمار­های دریافت کننده تیروکسین به شکل معنی­داری مقدار بالاتری چربی در مقایسه با هیپوفیز، LHRH و شاهد داشت. اسیدهای چرب اشباع اسید چرب C16:0 با مقادیر بین 44/28-16/21 درصد وزن خشک، اسید چرب C24:0 با محدوده­ی 60/19-15/16 درصد وزن خشک و اسید چرب C22:0 17/11-34/7 درصد وزن خشک فراوانترین اسیدهای چرب اشباع بودند. بالاترین میزان اسیدهای چرب اشباع (ΣSUF) با مقادیر 15/63 و 73/58 درصد وزن خشک به تیمار دریافت کننده هیپوفیز و تیمار دریافت کننده 50 میکروگرم و کمترین میزان این پارامتر با 55/54 و 14/50 درصد وزن خشک به تیمار شاهد و دریافت کننده LHRH تعلق داشت. دو تیمار هیپوفیز و تیمار دریافت کننده 50 میکروگرم وزن بدن تیروکسین، مجموع اسید چرب امگا 6 (Σω6) و 3 (Σω3) بالاتری داشتند (05/0p <). هورمون هیپوفیز و به دنبال آن هورمون تیروکسین با دوز 50 میکروگرم در مقایسه با دوزهای پایین تیروکسین و نیز LHRH کارایی بالاتری در افزایش سطح هورمون­های جنسی دخیل در فرآیند رسیدگی جنسی و نیز ترکیب اسیدهای چرب تخمدان ماهی مولد روهو دارند.

کلیدواژه‌ها


عنوان مقاله [English]

Comparative Study of Pituitary Extract, LHRH, and Thyroxine on Sex steroids, Histochemistry and (Labeo rohita) Producing Fatty Acids

نویسندگان [English]

  • Hadideh Mabudi
  • Ehsan Eslamizadeh
  • Laleh Roomiani
  • Mehran Javaheri Baboli
  • Mojdeh Cheleh Mal Dezfooli Nezhad
Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
چکیده [English]

This study aimed at assessing the comparative effect of pituitary hormones, LHRH, and thyroxine on hormonal indices and ovarian fatty acids of Labeo rohita. Blood samples were taken before injection, between the first and second injection, after the second injection and after ovulation, using a 5 ml plastic syringe from the tail stem. According to the measurement results, at the time between the first and second injections, after the second injection and after ovulation, the levels of estradiol, testosterone, 17-alpha hydroxy progesterone, cortisol and LH reached their peak levels in the pituitary and treatment receiving 50 μg dose had the lowest body weight in treatments receiving LHRH and control (P <0.05). Among thyroxine-receiving treatments, increasing thyroxine levels could significantly increase the hormones stimulating the maturation process (P <0.05). Pituitary receiving treatment and thyroxine receiving 50 μg per body weight had the highest amount of ovarian protein (P <0.05), with 16.70 and 15.99% dry weight and two LHRH and control treatments had the lowest ovarian protein level (P <0.05). Regarding fat, thyroxine-receiving treatments had a significantly higher amount compared to pituitary, LHRH, and control (P <0.05). The highest amount of saturated fatty acids (ΣSUF) with values ​​of 63.15 and 58.73% of dry weight to the pituitary receiving treatment and receiving 50 μg per body weight and the lowest amount of this parameter with 54.55 and 50.14% dry weight belonged to the control and LHRH recipient, respectively. C17: 1 and C18: 1n9c fatty acids in the range of 3.43-4.77 and 3.28-5.40% of dry weight and C24: 1n9 fatty acids in the range of 0.03-0.18% by dry weight had the highest and lowest levels of unsaturated fatty acids (ΣPUF), respectively. Both pituitary and 50 μg treatments of thyroxine had higher total omega-6 (Σω6) and 3 (Σω3) fatty acids (P <0.05). The results of the present study revealed that pituitary hormone followed by thyroxine at a dose of 50 μg per body weight compared to low doses of thyroxine and LHRH were more effective in increasing the level of sex hormones involved in the process of sexual maturation and the combination of acids. 

کلیدواژه‌ها [English]

  • Pituitary
  • LHRH
  • Thyroxine
  • Hormone
  • Fatty Acids
  • Labeo rohita
  1. Abdollahpour H., Falahatkar B., Efatpanah I., Meknatkhah B. Effect of intraperitoneal thyroxine injection on hematological indices in Sterlet sturgeon Acipenser ruthenus broodstock: a preliminary result. 8th International Symposium on Sturgeons, Vienna, Austria.
  2. Agha Mohammadpour P., Maboudi H., Javadzadeh N. 2017. The effect of salinity stress on growth rate, hematological parameters and survival of Arabibarbus grypus. Animal Physiology and Development, 45: 27-13.
  3. Ahmadnezhad M., Oryan S., Sahafi H.H., Khara H. 2013. Effect of Synthetic Luteinizing Hormone-Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisiikutum. Turkish Journal of Fisheries and Aquatic Sciences, 13: 95-100
  4. Al Zaidy F., Al-Noorm S., Jasim, B.M. 2017. The effect of hormone type and amount of dose on gonadotropin hormones levels in blood plasma of common carp (Cyprinus carpio) during artificial spawning processes. Iraqi Journal Aquaculture, 41(1): 41-28
  5. AOAC International 1995, Official methods of analysis of AOAC (International Association of Analytical Communities). Arlington, VA, USA.
  6. Bosak Kahkesh F., Yooneszadeh Feshalami M., Amiri F., Nickpey M. 2010. Effect of Ovaprim, Ovatide, HCG, LHRH-A2, LHRHA2+CPE and Carp Pituitary in Benni (Barbus sharpeyi) Artificial Breeding. Global Veterinaria, 5(4): 209-214.
  7. Cioffi F., Senese R., Petito G., Lasala P., de Lange P., Silvestri E., Lombardi A., Moreno M., Goglia F., Lanni, A. 2019. Both 3,3′ ,5-triiodothyronine and 3,5-diodo-L-thyronine Are Able to Repair Mitochondrial DNA Damage but by Different Mechanisms, Frontiers in Endocrinology, 10:216-225.
  8. Dhara K., Saha N.C. 2013. Controlled breeding of Asian catfish Clarias batrachus using pituitary gland extracts and ovaprim at different temperatures, latency periods and their early development. Journal of Aquaculture Research and Development, (4): 186-190.
  9. Drose S., Brandt U., Wittig I. 2014. Mitochondrial respiratory chain complexes as sources and targets of thiol-based redox-regulation. Biochimical et Biophysica Acta, 1844: 1344-1354.
  10. Eales J.G., Delvin R., Higgs D.A., Mcleese J.M., Oakes J.D., Plohman J. 2004. Thyroid function in growth hormone transgenic coho salmon (Oncorhynchus kisutch). Canadian Journal of Zoology, 82: 1225-1229.
  11. Ebrahimi G., Virtual Amiri B., Rafiei G., Shabani A. 2016. Histological and biochemical characteristics of mature oocytes of Iranian sturgeon breeders (Acipenser persicus). Fisheries Iranian Journal of Natural Resources. 70: 203-189.
  12. Figueira TR, BarrosMH, Camargo AA, Castilho RF, Ferreira JC, Kowaltowski AJ., 2013. Mitochondria as a source of reactive oxygen and nitrogen species: from molecular mechanisms to human health. Antioxid Redox Signal, 18: 2029-2074.
  13. Flood D.E.K., Fernandino J.I., Langlois V.S. 2013. Thyroid hormones in male reproductive development: Evidence for direct crosstalk between the androgen and thyroid hormone axes. Genernal and Comparative Endocrinology, 192: 2-14
  14. Habibi H.R., Nelson E.R., Allan E.R.O. 2010. New insights into thyroid hormone function and modulation of reproduction in goldfish. Genernal and Comparative Endocrinology, 175: 19-26
  15. Henderson R.J., Tocher, D.R., 1987. The lipid composition and biochemistry of freshwater fish. Progress Lipid Research, 26: 281-347.
  16. Heraedi A., Prayitno S.B., Yuniarti T. 2018. The Effect of Different Thyroxine Hormone (T4) Concentration on The Growth, Survival, and Pigment Development of Pink Zebra Fish Larvae (Brachydanio reiro). Omni-Akuatika, 14(2): 21-28
  17. Imanpoor M.R., Taghizadeh V., Khodadoust A., Roohi Z. 2018. Effect of fish size and seasonal changes on gonadal steroid hormones in pike brood stocks (Esox lucius). Nova Biologica Reperta, 5: 65-71.
  18. Ismail R., Mourad M.M., Negm R.N., Assem S.S. 2017. Effect of prolonged exposure to thyroxine on growth, puberty timing and ovarian structure in female red tilapia (Oreochromis sp.). Egyptian Journal of Aquatic Research, 3: 313–320.
  19. Jamili S., Toloui M.H., Qanatparast Q., Jazbi Zadeh A. 2006. Investigation of the effect of thyroxine hormone on female sexual predisposition and acceleration of growth and development of eggs and larvae of rainbow trout, Amur, Phytophagous and Caspian fish sauce. Iran Fisheries Research Institute, 5: 22-30.
  20. Jasemzadeh A., Maboudi H., Askarizazi A., Basak Kahkesh F. 2016. Comparison of the effect of evaprim hormone and pituitary gland extract of carp on reproductive indices of phytophagous fish (Hipophthalmichthys molitrix). Journal of New Technologies in Aquaculture Development, 10: 1-10.
  21. Jerez S., Rodríguez C., Cejas J.R., Bolaños A., Lorenzo A. 2006. Lipid dynamics and plasma level changes of 17 β-estradiol and testosterone during the spawning season of gilthead seabream (Sparus aurata) females of different ages. Comparative Biochemistry and Physiology, 143: 180-189.
  22. Kagawa H., Young G., Naghama Y. 1984. Invitro estradiol-17β and testosterone production by ovarian follicls of gold fish (carassius carassius). General and Comparative Endocrinology, 54:139-143.
  23. Kashani Sabet A., Eryan Sh., Bahmani M. 2004. Induction of ovulation in phytophage generators (Hypophthalmichthys molitrix) using LHRH-A and its combination with dopamine antagonists. Iranian Journal of Fisheries, 13: 144-129.
  24. Khalil N.A., Allah H.M.K., Mousa M. A. 2011. The effect of maternal thyroxine injection on growth, survival and development of the digestive system of Nile tilapia (Oreochromis niloticus) larvae. Advances in Bioscience and Biotechnology, 2: 320-329.
  25. Khormian S., Kochinin P., Yavari W., Solati A.P. 2019. The effect of hormonal treatments on spawning and quality of larvae produced by Acanthopagrus arabicus. Iranian Journal of Fisheries, 28: 46-37.
  26. Leatherland J., Barret S. 1993. Investigation into the development of the pituitary gland _ thyroid tissue axis and distribution of tissue thyroid hormone content in embryonic coho salmon (Oncorhynchus kisutsch) from Lake Ontario. Fish Physiology and Biochemistry, 12: 149-159
  27. Maboudi H., Javadzadeh N., Savari A., Taghia Azhir M. 2016. Comparison of the effect of three-stage injection of pituitary gland extract and LHRHa2 hormone with two-stage injection of pituitary extract on some tissue, reproductive and sex steroids of male and female Arabibarbus grypus. Journal of Animal Physiology and Development, 11: 26-17.
  28. Mortazavizadeh S.A., Amiri F., Youneszadeh Fashalami M., Hosseinzadeh Sahafi H., Hooshmand H. 2013. Comparison of replacement ratio of Indian carp with conventional carp in earthen pond of Khuzestan province. Iranian Journal of Fisheries, 22: 128-117.
  29. Naeem M., Zunber A., Ashraf M., Ahmad W., Ishtiaq A., Najam-ul-Hasan H. 2013. Induced breeding of Labeo rohita through single application of ovaprim-C at Faisalabad Hatchery, Pakistan. African Journal of Biotechnology,12: 2722-2726.
  30. Piñuela C., Rendón C., Gonzalez de Canales M.L., Sarasquete C. 2004. Development of the Senegal sole, (Solea senegalensis) forebrain. European Journal of Histochemical, 48: 377-384
  31. Rankin J.C., pitcher T.J., Duggan R.T. 1983. Control processes in fish physiology. Croom Helm. 298p
  32. Scheibye-Knudsen M., Fang E.F., Croteau D.L., Wilson D.M., Bohr V.A. 2015· Protecting the mitochondrial powerhouse. Trends in Cell Biology, 25:158-170
  33. Schmid A.C., Lutz I., Kloas W., Reinecke M. 2003. Thyroid hormone stimulates hepatic IGF-I mRNA expression in a bony fish, the tilapia (Oreochromis mossambicus) in vitro and in vivo. General and Comparative Endocrinology, 130: 129-134.
  34. Serajian S., Zamini A.A., Yousefian M., Saeedi A.A., Jafari, A. 2007. Comparative study of serum levels of some sex steroid hormones in preterm and adult breeders of Caspian golden mullet (Liza auratus). Fisheries Magazine, 3: 1-9.
  35. Taghizadeh V., Imanpoor M.R., Mehdinejad N. 2013. Study the seasonal steroid hormones of common carp in Caspian Sea. Iran. World Journal of Fish and Marine Sciences, 5: 282-285
  36. Teymouri M., Mohammadi Zadeh F., Bahri A. 2016. Artificial reproduction of serum (Cichlasomaseverum sp) using carp pituitary hormone extract (CPE) and synthetic hormone GnRHa. Journal of Aquaculture Development, 12: 40-31.
  37. Tocher D.R. 2003. Metabolism and functions of lipids and fatty acids in teleost fish. Reviews in Fisheries Science, 11: 107-184
  38. Tocher D.R. 2010. Fatty acid requirements in ontogeny of marine and freshwater fish. Aquaculture Research, 41: 717-732
  39. Tovo-Neto A., Rodrigues M., Habibi H., Nobrega R. 2018. Thyroid hormone actions on male reproductive system of teleost fish. Genernal and Comparative Endocrinology, 265: 230-236
  40. Urbinati E.C., Vasques L.H., Senhorini J.A., Souza V.L., Gonçalves F.D. 2008. Larval performance of matrinxã, (Brycon amazonicus), after maternal triiodothyronine injection or egg immersion. Aquaculture Research, 39: 1355-1359.