تأثیر هشت هفته ورزش استقامتی، وانادیوم و عصاره استویا بر تغییرات ساختاری عضله همسترینگ موش‌های نر دیابتی نوع یک

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه صنعتی شاهرود، سمنان، ایران

2 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه دامغان، سمنان، ایران

چکیده

کاهش توده عضلانی یکی از عوارض جانبی بیماری دیابت می­باشد که ممکن است از طریق تنظیم مقادیر قند خون توسط ورزش و یا مکمل­های گیاهی به تاخیر بیفتد.  تعداد 35 سر موش نر (10±180گرم)، به 7گروه: کنترل، دیابتی، دیابتی+تمرین، دیابتی+ وانادیوم، دیابتی+ تمرین+ وانادیوم، دیابتی+ استویا و دیابتی+ تمرین+ استویا تقسیم شدند. جهت القای دیابت نوع1، استرپتوزوتوسین (55 میلی­گرم/کیلوگرم) درون صفاقی تزریق شد، و عصاره وانادیوم (1میلی­گرم/میلی­لیتر) در آب روزانه محلول گردید. برنامه تمرین استقامتی شامل هشت هفته دویدن، و 5روز در هفته بود، که با زمان 15 دقیقه و سرعت 20 متر/دقیقه آغاز، و به 35 دقیقه و سرعت 30 متر/دقیقه در هفته پایانی رسید. تغییرات هیستوپاتولوژی در بافت کبد با استفاده از میکروسکوپ نوری بررسی گردید. به­منظور نرمال­سازی داده­های پارامتریک از آزمون کلموگروف­اسمیرنف و جهت تجزیه تحلیل آن­ها، آزمون یک طرفه آنوا با تست تعقیبی بونفرونی، و برای داده­های ناپارامتریک آزمون کروسکال والیس (05/0≥ p) بکار گرفته شد. : مقادیر وزن بدن در هر شش گروه تجربی در مقایسه با گروه کنترل سالم (001/0= p)، در گروه وانادیوم+ورزش در مقایسه با وانادیوم (01/0= p)، و در گروه استویا+ورزش در مقایسه با استویا (04/0=p )، کاهش معناداری یافت. در گروه دیابتی، تغییرات خفیف تعداد و اندازه سلول­ها، با اجرای ورزش و همراه با وانادیوم و استویا، و مصرف استویا، و نیز دژنراسیون سلول­ها، توسط ورزش و همراه با وانادیوم و استویا، و مصرف وانادیوم و استویا بهبود پیدا کرد (001/0=p ). به­­نظر می­رسد که اجرای هشت هفته فعالیت استقامتی، مصرف استویا و وانادیوم بر تنظیم وزن بدن و نیز بهبود تخریبات همسترینگ ناشی از دیابت موثر باشند.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 8 Weeks of Endurance Exercise, Consumption of Vanadium and Stevia on Structural Changes in the Hamstring Muscle of Type 1 Diabetic Rats

نویسندگان [English]

  • Atefeh Sarhadi 1
  • Ali Hassani 1
  • Maliheh Ardakanizadeh 2
1 Department of Exercise Physiology, Faculty of Sport Sciences, Shahrood University of Technology, Semnan, Iran
2 Department of Sport Sciences, Faculty of Human Sciences, Damghan University, Semnan, Iran
چکیده [English]

Loss of muscle mass is one of the side effects of diabetes that may be delayed by regulating blood sugar levels through exercise or herbal supplements. Thirty-five male rats (180±10 g) were assigned to seven groups of control, diabetic, diabetic + exercise, diabetic + vanadium, diabetic + exercise + vanadium, diabetic + stevia, and diabetic + exercise + stevia. To induce type 1 diabetes, streptozotocin (55 mg/kg) was injected intraperitoneally, and vanadium extract (1mg/ml) was daily dissolved in water. The endurance training program consisted of eight weeks of treadmill running, five days a week, starting at 15 minutes at 20 meters/minute and ending at 35 minutes at 30 meters/minute in the final week. Histopathological changes in brain tissue were examined using a light microscope. In order to normalize the parametric data, Kolmogorov-Smirnov test and for their analysis, one-way ANOVA with Bonferroni post hoc test, and for non-parametric data, Kruskal-Wallis test (p ≤ 0.05) were used. Body weight values ​​in all six experimental groups significantly decreased compared to healthy control group (p=0.001), in vanadium group compared to vanadium + exercise (p = 0.01), and in stevia + exercise group compared to stevia (p = 0.04). In the diabetic group, slight changes in cell number and size improved with exercise along with vanadium and stevia, and consumption of stevia, as well as cell degeneration with exercise along with vanadium and stevia, and consumption of vanadium and stevia (p=0.001). Eight weeks of endurance exercise, stevia, and vanadium intake appear to be effective in regulating body weight as well as improving the hamstring muscle damage caused by diabetes.
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کلیدواژه‌ها [English]

  • Endurance training
  • Vanadium
  • Stevia
  • Hamstring Muscle
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