اثر گالیک اسید بر حافظه اجتنابی غیر فعال، حافظه کاری و تراکم نورون‌های تیره نواحی CA1/CA3 در مدل دژنراسیون هیپوکامپ موش صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه زیست‌شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران

چکیده

مسمومیت با تری‌متیل‌تین (TMT) با القاء دژنراسیون هیپوکامپ سبب بروز آسیب شناختی و تولید نورون‌های تیره در مناطق مختلف هیپوکامپ می­‌شود. مطالعه حاضر اثر اسید گالیک (GA) را بر حافظه کاری، حافظه اجتنابی، و تراکم نورون‌های تیره در نواحی CA1/CA3 هیپوکامپ موش‌های صحرایی در پی مسمومیت با TMT ارزیابی می­کند. در این مطالعه، تعداد 32 سر موش صحرایی نر بالغ نژاد ویستار به صورت تصادفی در 4 گروه شامل کنترل، TMT+NS، TMT+GA100 و TMT+GA200 قرار گرفتند. اسید گالیک با دوزهای  100 و 200 میلی‌گرم بر کیلوگرم وزن بدن حیوان، 24 ساعت پس از مسمومیت با TMT (mg/kg 8) به صورت دهانی تجویز شد. جهت ارزیابی حافظه کاری از ماز Y و جهت سنجش حافظه اجتنابی از جعبه شاتل استفاده شد. آنگاه تراکم نورون‌های تیره در نواحی CA1 و  CA3 هیپوکامپ به روش دایسکتور بررسی شد. همچنین، به منظور تعیین وجود اختلاف معنا‌دار بین گروه­های مورد نظر، از آزمون آنالیز واریانس یک طرفه و آزمون تعقیبی توکی استفاده شد و از نظر آماری مقادیر 05/0 > p  معنادار در نظر گرفته شد. کاهش معنی‌دار درصد رفتار تناوبی، تاخیر ورود به محفظه تاریک شاتل باکش، به همراه افزایش تراکم نورون‌های تیره در گروه­ TMT+NS نسبت به گروه کنترل دیده شد (001/0 > p). در حالی که تجویز گالیک اسید سبب بهبود حافظه کاری و اجتنابی و کاهش تراکم نورون‌های تیره CA1/CA3 هیپوکامپ نسبت به گروه TMT+NS گردید (001/0˂p). به نظر می‌رسد تجویز اسید گالیک با کاهش میزان آسیب دیدگی نورون‌های نواحی CA1/CA3 هیپوکامپ سبب بهبود علائم شناختی به دنبال مسمومیت با TMT می‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Gallic Acid on Passive Avoidance Memory, Working Memory, and Dark Neuron Cell Density in CA1/CA3 Areas in Rats Hippocampal Degeneration Model

نویسندگان [English]

  • Seyed Kamaladdin Yazdanfar
  • Mohammad Amin Edalatmanesh
  • Seyed Ebrahim Hosseini
Department of Biology, Faculty of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
چکیده [English]

Trimethyltin (TMT) intoxication with hippocampal degeneration induces the production of dark neurons in different areas of the hippocampus. The present study assessed the effect of Gallic acid (GA) on working memory, avoidance memory, and the density of dark neurons in the CA1/CA3 regions of the rat hippocampus following TMT intoxication. In this study, 32 adult male Wistar rats were randomly assigned to four groups including control, TMT+NS, TMT+GA100, and TMT+GA200. GA at doses of 100 and 200 mg/kg per body weight was administered orally 24 hours after TMT injection (8 mg/kg). The Y-maze was used to assess the working memory and the shuttle box was used to measure avoidance memory. The density of dark neurons in CA1 and CA3 regions of the hippocampus was then assessed by dissector method. Moreover, in order to determine the existence of significant differences between the groups, one-way ANOVA and Tukey’s post hoc test were used and p <0.05 was considered statistically significant. There was a significant decrease in the percentage of alteration behavior, delay in entering the dark room of shuttle box, along with an increased density of dark neurons in the TMT+NS group compared to the control group (p<0.001). While, administration of GA ameliorated the working and avoidance memory and reduced the density of CA1/CA3 dark neurons in the hippocampus compared to TMT+NS group (p˂0.001). GA administration appears to improve cognitive symptoms following TMT intoxication by reducing neuronal damage to CA1/CA3 areas of the hippocampus.
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کلیدواژه‌ها [English]

  • Trimethyltin
  • Gallic acid
  • Hippocampus
  • Rat
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